Technical Papers
Aug 25, 2022

Production of Self-Healing Concrete by Bacillus subtilis Spores Immobilized on Sand Using Gum Arabic as an Adhesion Material: Proof of Concept

Publication: Journal of Materials in Civil Engineering
Volume 34, Issue 11

Abstract

Production of self-healing concrete involves the immobilization of a bacterium and calcium-based bacterial nutrient on a carrier material before being added to the concrete mixture. The objective of this paper was to test the potential of using gum arabic of Acacia senegal (Hashab) to immobilize Bacillus subtilis on coarse sand for production of self-healing concrete. Dried bacterial spores, gum arabic powder, and calcium lactate were mixed thoroughly in a 1-L flask. The mixture was wetted by adding tap water gradually until spore-coated sand pellets were formed and then left to dry. Three mortar mixtures with different concentrations (2.5%, 5%, and 10%) of spore-coated sand pellets were then prepared and left for 24 h at room temperature. Of these, 12 mortar prisms were cast. Prisms were subjected to three-point load testing under a controlled and careful rate of loading until visible cracks appeared on the surface of the prism. Crack healing was then monitored for 14 days using stereoscopy and a digital image camera under three curing regimes, namely wet, wet–dry cycles, and polyethylene sheets. Results showed that the efficiency of healing increased with an increase in the percentage of spore-coated sand pellets in the mix without causing a significant impact on prisms strength for mixes with 2.5% and 5% of pellets. The results give an evidence to use gum arabic as a novel, abundant, and sustainable material for immobilization of bacteria on sand surface to produce self-healing concrete.

Get full access to this article

View all available purchase options and get full access to this article.

Data Availability Statement

All data, models, and code generated or used during the study appear in the published article.

Acknowledgments

The authors would like to thank Veterinary Medicine College, University of Bahri, and the Faculty of Pharmacy, University of Karary, for providing the bacteria spores and broth. Also, thanks go to the Material Research Centre (MRC) for conducting the XRD test.

References

Afiq, M., H. Abdullah, N. Ain, and H. Abdullah. 2018. “Development and performance of bacterial self-healing concrete—A review.” Mater. Sci. Eng. 431 (6): 062003. https://doi.org/10.1088/1757-899X/431/6/062003.
Algaifi, H., S. A. Bakar, A. R. M. Sam, M. Ismail, A. R. Z. Abidin, S. Shahir, and W. A. H. Altowayti. 2020. “Insight into the role of microbial calcium carbonate and the factors involved in self-healing concrete.” Constr. Build. Mater. 254 (Sep): 119258. https://doi.org/10.1016/j.conbuildmat.2020.119258.
Algaifi, H. A., S. A. Bakar, R. Alyousef, A. R. M. Sam, M. W. Ibrahim, S. Shahidan, M. Ibrahim, and B. A. Salami. 2021. “Bio-inspired self-healing of concrete cracks using new B. pseudomycoides species.” J. Mater. Res. Technol. 12 (May): 967–981. https://doi.org/10.1016/j.jmrt.2021.03.037.
Al-Tabbaa, A., C. Litina, P. Giannaros, A. Kanellopoulos, and L. Souza. 2019. “First UK field application and performance of microcapsule-based self-healing concrete.” Constr. Build. Mater. 208 (May): 669–685. https://doi.org/10.1016/j.conbuildmat.2019.02.178.
Alves, L., L. Alves, M. Mello, and S. Barros. 2019. “Characterization of bioconcrete and the properties for self-healing.” Proceedings 38 (1): 4. https://doi.org/10.3390/proceedings2019038004.
BSI (British Standard Institution). 1985. Testing aggregates Part 1: Methods of determination of particle size distribution. BS 812. London: BSI.
BSI (British Standard Institution). 1992. Specification of aggregate from natural sources for concrete. BS 882. London: BSI.
BSI (British Standard Institution). 2005. Methods of testing cement. Determination of strength. BS EN 196-1. London: BSI.
De Belie, N., et al. 2018. “A review of self-healing concrete for damage management of structures.” Adv. Mater. Interfaces 5 (17): 1800074. https://doi.org/10.1002/admi.201800074.
Feng, J., B. Chen, W. Sun, and Y. Wang. 2021. “Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization.” Constr. Build. Mater. 280 (Apr): 122460. https://doi.org/10.1016/j.conbuildmat.2021.122460.
Griño, A. A., M. Daly, M. Klarissa, and J. M. C. Ongpeng. 2020. “Bio-influenced self-healing mechanism in concrete and its testing: A review.” Appl. Sci. 10 (15): 5161. https://doi.org/10.3390/app10155161.
Gupta, S., and K. H. Wei. 2016. “Encapsulation technology and techniques in self-healing concrete.” J. Mater. Civ. Eng. Am. Soc. Civ. Eng. 28 (12): 04016165. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001687.
Jadhav, U. U., M. Lahoti, Z. Chen, J. Qiu, B. Cao, and E. H. Yang. 2018. “Viability of bacterial spores and crack healing in bacteria-containing geopolymer.” Constr. Build. Mater. 169 (30): 716–723. https://doi.org/10.1016/j.conbuildmat.2018.03.039.
Jonkers, H. M. 2011. “Bacteria-based self-healing concrete.” Constr. Build. Mater. 56 (1): 1–12.
Jonkers, H. M., A. Thijssen, G. Muyzer, O. Copuroglu, and E. Schlangen. 2010. “Application of bacteria as self-healing agent for the development of sustainable concrete.” Ecol. Eng. 36 (2): 230–235. https://doi.org/10.1016/j.ecoleng.2008.12.036.
Khaliq, W., and M. B. Ehsan. 2016. “Crack healing in concrete using various bio influenced self-healing techniques.” Constr. Build. Mater. 102 (Jan): 349–357. https://doi.org/10.1016/j.conbuildmat.2015.11.006.
Muhammad, N. Z., et al. 2016. “Tests and methods of evaluating self-healing efficiency of concrete: A review.” Constr. Build. Mater. 112 (May): 1123–1132. https://doi.org/10.1016/j.conbuildmat.2016.03.017.
Neville, A. M., and J. J. Brooks. 2010. Concrete technology. 2nd ed. London: Pearson Education.
Satti, S. A. A., and Y. H. Ahmed. 2018. “Use of gum arabic (Acacia seyal) as concrete admixture.” In Proc., Conf. Civil Engineering 2018, 198–202. Khartoum, Sudan: Univ. of Khartoum.
Seifan, M., A. K. Samani, and A. Berenjian. 2016. “Bioconcrete: Next generation of self-healing concrete.” Appl. Microbiol. Biotechnol. 100 (6): 2591–2602. https://doi.org/10.1007/s00253-016-7316-z.
Shaheen, N., R. Arsalan, W. Khaliq, H. Murtaza, and R. Iqbal. 2019. “Synthesis and characterization of bio-immobilized nano/micro inert and reactive additives for feasibility investigation in self-healing concrete.” Constr. Build. Mater. 226 (Nov): 492–506. https://doi.org/10.1016/j.conbuildmat.2019.07.202.
Talaiekhozan, A., and M. Z. Abd Majid. 2014. “A review of self-healing concrete research development.” J. Environ. Treatment Tech. 2 (1): 1–11.
Uchechukwu, A., G. Abdulbasir, and G. Abdulkadir. 2018. “Gum arabic as an admixture for cement concrete production.” Constr. Build. Mater. 176 (Apr): 201–212. https://doi.org/10.1016/j.conbuildmat.2018.04.160.
Vermeer, C. M., E. Rossi, J. Tamis, H. M. Jonkers, and R. Kleerebezem. 2021. “Resources, conservation & recycling from waste to self-healing concrete: A proof-of-concept of a new application for polyhydroxyalkanoate.” Resour. Conserv. Recycl. 164 (Mar): 105206. https://doi.org/10.1016/j.resconrec.2020.105206.
Wang, J. Y., N. De Belie, and W. Verstraete. 2012. “Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete.” J. Ind. Microbiol. Biotechnol. 39 (4): 567–577. https://doi.org/10.1007/s10295-011-1037-1.
Wang, J. Y., H. Soens, W. Verstraete, and N. De Belie. 2014. “Self-healing concrete by use of microencapsulated bacterial spores.” Cem. Concr. Res. 56 (Nov): 139–152. https://doi.org/10.1016/j.cemconres.2013.11.009.

Information & Authors

Information

Published In

Go to Journal of Materials in Civil Engineering
Journal of Materials in Civil Engineering
Volume 34Issue 11November 2022

History

Received: Oct 9, 2021
Accepted: Mar 16, 2022
Published online: Aug 25, 2022
Published in print: Nov 1, 2022
Discussion open until: Jan 25, 2023

Permissions

Request permissions for this article.

Authors

Affiliations

Associate Professor, Dept. of Civil Engineering, Faculty of Engineering, Univ. of Khartoum, P. O. Box 321, Khartoum 51111, Sudan (corresponding author). ORCID: https://orcid.org/0000-0002-3116-6834. Email: [email protected]
Ahmed A. Ibrahim [email protected]
Master’s Student, Dept. of Civil Engineering, Faculty of Engineering, Univ. of Khartoum, P. O. Box 321, Khartoum 51111, Sudan. Email: [email protected]
Khadija E. Elamin [email protected]
Master’s Student, Microbial Culture Collection Unit (MCCU), Faculty of Science, Univ. of Khartoum, P.O. Box 321, Khartoum 51111, Sudan. Email: [email protected]
Adil A. El Hussein [email protected]
Professor, Microbial Culture Collection Unit (MCCU), Faculty of Science, Univ. of Khartoum, P.O. Box 321, Khartoum 51111, Sudan. Email: [email protected]
Marmar A. El Siddig [email protected]
Professor, Microbial Culture Collection Unit (MCCU), Faculty of Science, Univ. of Khartoum, P.O. Box 321, Khartoum 51111, Sudan. Email: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share