Effect of Using Calcium Lactate with Bacillus pseudofirmus Bacteria on Self-Healing Efficiency of Bacterial Concrete
Publication: Tran-SET 2022
ABSTRACT
This study adopted the vacuum impregnation of bacteria into a lightweight aggregate (LWA) technique by using yeast extract as a nutrient, calcium lactate as a mineral precursor, and bacteria spores called Bacillus pseudofirmus as a healing agent. The impregnated lightweight aggregate was used in the concrete mixture to evaluate the effect of calcium lactate with bacteria on the mechanical properties of concrete and crack healing efficiency. In addition, a control sample was prepared without bacteria or precursors for comparison purposes. Three concrete cylinders and three concrete beams were prepared for each concrete mixture. The results showed that the concrete mixture with calcium lactate and Bacillus pseudofirmus bacteria (N + CaL + BP) significantly improved the flexural strength, compressive strength, and flexural strength recovery compared to the control sample. However, it did not significantly increase the self-healing efficiency (16.11% only increase in self-healing efficiency of side faces of N + CaL + BP compared to control).
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REFERENCES
Bhavya, K., and Sanjeev, D. N. (2017). Effect of Different Types of Coarse Aggregates on Physical Properties of Mostly Used Grades M20, M25, M30 of Concrete. IOSR Journal of Mechanical and Civil Engineering, 14(01), 46–51. https://doi.org/10.9790/1684-1401024651.
Brinkman, L., and Miller, S. A. (2021). Environmental impacts and environmental justice implications of supplementary cementitious materials for use in concrete. Environmental Research: Infrastructure and Sustainability, 1(2), 025003. https://doi.org/10.1088/2634-4505/ac0e86.
Castro-Alonso, M. J., Montañez-Hernandez, L. E., Sanchez-Muñoz, M. A., Macias Franco, M. R., Narayanasamy, R., and Balagurusamy, N. (2019). Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete: Microbiological and molecular concepts. Frontiers in Materials, 6(June), 1–15. https://doi.org/10.3389/fmats.2019.00126.
DOTD, L. (2016). Specifications of Louisiana.
Erşan, Y. Ç., Hernandez-Sanabria, E., Boon, N., and De Belie, N. (2016). Enhanced crack closure performance of microbial mortar through nitrate reduction. Cement and Concrete Composites, 70, 159–170. https://doi.org/10.1016/j.cemconcomp.2016.04.001.
Hearn, N. (1998). Self-sealing, autogenous healing and continued hydration: What is the difference? Materials and Structures/Materiaux et Constructions, 31(8), 563–567. https://doi.org/10.1007/bf02481539.
Hungria, R., Mousa, M., Hassan, M., Omar, O., Gavilanes, A., Arce, G., Milla, J., and King, G. (2021). Effect of Using Magnesium Acetate on the Self-Healing Efficiency of Hydrogel-Encapsulated Bacteria in Concrete. Tran-SET 2021 - Proceedings of the Tran-SET Conference 2021, 163–175. https://doi.org/10.1061/9780784483787.018.
Mors, R. M., and Jonkers, H. M. (2017). Feasibility of lactate derivative based agent as additive for concrete for regain of crack water tightness by bacterial metabolism. Industrial Crops and Products, 106, 97–104. https://doi.org/10.1016/j.indcrop.2016.10.037.
Omar, O., Mousa, M., Hassan, M., Hungria, R., Gavilanes, A., Arce, G., Milla, J., & Rupnow, T. (2021). Vacuum Impregnation of Bacillus pseudofirmus into Fine Lightweight Aggregate. Tran-SET 2021 - Proceedings of the Tran-SET Conference 2021, 365–372. https://doi.org/10.1061/9780784483787.036.
Rooij, D. R. M., and Schlange, E. (2011). Self-healing phenomena in cement-based materials..
Schreiberová, H., Bílý, P., Fládr, J., Šeps, K., Chylík, R., & Trtík, T. (2019). Impact of the self-healing agent composition on material characteristics of bio-based self-healing concrete. Case Studies in Construction Materials, 11. https://doi.org/10.1016/j.cscm.2019.e00250.
Sisomphon, K., Copuroglu, O., and Fraaij, A. (2011). Application of encapsulated lightweight aggregate impregnated with sodium monofluorophosphate as a self-healing agent in blast furnace slag mortar. Heron, 56(1–2), 17–36.
Soysal, A., Milla, J., King, G. M., Hassan, M., and Rupnow, T. (2020). Evaluating the Self-Healing Efficiency of Hydrogel-Encapsulated Bacteria in Concrete. Transportation Research Record, 2674(6), 113–123. https://doi.org/10.1177/0361198120917973.
Standard Practice for Making and Curing Concrete Test Specimens in the Laboratory. (n.d.). Retrieved June 17, 2022, from https://www.astm.org/c0192_c0192m-19.html
Standard Test Method for Compressive Strength of Cylindrical Concrete Specimens. (n.d.). Retrieved June 24, 2022, from https://www.astm.org/c0039_c0039m-14.html
Standard Test Method for Flexural Performance of Fiber-Reinforced Concrete (Using Beam With Third-Point Loading). (n.d.). Retrieved June 17, 2022, from https://www.astm.org/c1609_c1609m-12.html
Wiktor, V., and Jonkers, H. M. (2011). Quantification of crack-healing in novel bacteria-based self-healing concrete. Cement and Concrete Composites, 33(7), 763–770. https://doi.org/10.1016/j.cemconcomp.2011.03.012.
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Published online: Dec 13, 2022
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