Experimental Optimization of Microbial-Induced Carbonate Precipitation for Soil Improvement
Publication: Journal of Geotechnical and Geoenvironmental Engineering
Volume 139, Issue 4
Abstract
Implementation of laboratory-tested biomediated soil improvement techniques in the field depends on upscaling the primary processes and controlling their rates. Microbial-induced carbonate precipitation (MICP) holds the potential for increasing the shear stiffness and reducing the hydraulic conductivity by harnessing a natural microbiological process that precipitates calcium carbonate. The study presented herein focuses on controlling MICP treatment of one-dimensional flow, half-meter-scale column experiments. Treatment was optimized by varying procedural parameters in five pairs of experiments including flow rates, flow direction, and formulations of biological and chemical amendments. Monitoring of column experiments included spatial and temporal measurements of the physical, chemical, and biological properties essential to the performance of MICP, including shear wave velocity, permeability, calcium carbonate content, aqueous calcium, aqueous ammonium, aqueous urea, and bacterial density. Relatively uniform improvement of a half-meter one-dimensional flow sand column experiment resulted in a change from a shear wave velocity of 140 m/s to an average of 600 m/s. Examination of data sets provides insight into which parameters have a first-order effect of MICP treatment uniformity and efficiency and how these parameters can be monitored and controlled.
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Acknowledgments
Funding for the current study was provided by Geosyntec Consultants Inc., the Univ. of California Discovery Grant Program, and the National Science Foundation (Grant Nos. 0727463 and 0628782). The assistance of Dr. Nelson in developing and refining the microbiological procedures is especially appreciated. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the writer(s) and do not necessarily reflect the views of the National Science Foundation.
References
Al Qabany, A., Mortensen, B., Martinez, B., Soga, K., and DeJong, J. (2011). “Microbial carbonate precipitation: Correlation of S-wave velocity with calcite precipitation.” Proc., ASCE Geo-Frontiers 2011 Conf., ASCE, Reston, VA.
ASCE. (2006). “Report card of America's infrastructure. ”〈http://www.asce.com/reportcard〉 (Oct. 11, 2010).
Barkouki, T., et al. (2010). “Forward and inverse bio-geochemical modeling of microbially induced calcium carbonate precipitation in half-meter column experiments.” Transp. Porous Media, 90(1), 23–29.
DeJong, J., et al. (2009). “Upscaling of bio-mediated soil improvement.” Proc., 17th Int. Conf. on Soil Mechanics and Geotechnical Engineering, Millpress Science, Rotterdam, Netherlands, 2300–2303.
DeJong, J. T., et al. (2010a). “Soil engineering in vivo: Harnessing natural biogeochemical systems for sustainable, multi-functioning engineering solutions.” J. Roy. Soc. Interface, 8(1), 1–15.
DeJong, J. T., Fritzges, M. B., and Nusslein, K. (2006). “Microbial induced cementation to control sand response to undrained shear.” J. Geotech. Geoenviron. Eng., 132(11), 1381–1392.
DeJong, J. T., Mortensen, B. M., Martinez, B. C., and Nelson, D. C. (2010b). “Bio-mediated soil improvement.” Ecol. Eng., 36(2), 197–210.
Ferris, F. G., Stehmeier, L. G., Kantzas, A., and Mourits, F. M. (1996). “Bacteriogenic mineral plugging.” J. Can. Petroleum Technol., 35(8), 56–61.
Fidaleo, M., and Lavecchia, R. (2003). “Kinetic study of enzymatic urea hydrolysis in the pH range 4-9.” Chem. Biochem. Eng., 17(4), 311–318.
Fujita, Y., et al. (2008). “Stimulation of microbial urea hydrolysis in groundwater to enhance calcium carbonate precipitation.” Environ. Sci. Technol., 42(8), 3025–3032.
Fujita, Y., Redden, G. D., Ingram, J. C., Cortez, M. M., Ferris, F. G., and Smith, R. W. (2004). “Strontium incorporation into calcium carbonate generated by bacterial ureolysis.” Geochim. Cosmochim. Acta, 68(15), 3261–3270.
Ginn, T. R., Wood, B. D., Nelson, K., Scheibe, T. D., Murphy, E. M., and Clement, T.P. (2002). “Processes in microbial transport in the natural subsurface.” Adv. Water Res., 25 (8–12): 1017–1042.
Harkes, M. P., Booster, J. L., van Paassen, L. A., and van Loosdrecht, M. C. M. (2008) “Microbial induced carbonate precipitation as ground improvement method: Bacterial fixation and emperical correlation CsCO3 vs strength.” Proc., 1st Int. Conf. on Bio-Geo-Civil Engineering, 7–41, TU Delft, Delft, Netherlands.
Knorst, M. T., Neubert, R., and Wohlrab, W. (1997). “Analytical methods for measuring urea in pharmaceutical formulations.” J. Pharm. Biomed. Anal., 15(11), 1627–1632.
Mansell, B. O., de Vellis, L., and Schroeder, E. D. (2000). “Automated separation and conductimetric determination of inorganic nitrogen.” J. Environ. Eng., 126(8), 778–780.
Martinez, B. C., and DeJong, J. T. (2009). “Bio-mediated soil improvement: Load transfer mechanisms at the micro- and macro-scales.” US-China Workshop on Ground Improvement Technologies, ASCE, Reston, VA, 242–251.
Montoya, B. M., et al. (2012). “Fabrication, operation, and health monitoring of bender elements for aggressive environments.” ASTM Geotech. Test. J., 35(5).
Ramachandran, S. K., Ramakrishnan, V., and Bang, S. S. (2001). “Remediation of concrete using micro-organisms.” ACI Mater. J., 98(1), 3–9.
Ramakrishnan, V., Bang, S. S., and Deo, K. S. (1998). “A novel technique for repairing cracks in high performance concrete using bacteria.” Proc., Int. Conf. on High Performance High Strength Concrete, Curtin Univ. of Technology, Perth, Australia, 597–618.
Renforth, P., Manning, D. A., and Lopez-Capel, E. (2009). “Carbonate precipitation in artificial soils as a sink for atmospheric carbon dioxide.” Appl. Geochem., 24(9), 1757–1764.
Stocks-Fischer, S., Galinat, J. K., and Bang, S. S. (1999). “Microbiological precipitation of CaCO3.” Soil Biol. Biochem., 31(11), 1563–1571.
van Paassen, L. A., Harkes, M. P., van Zweiten, G. A., van der Zon, W. H., van der Star, W. R. L., and van Loosdrecht, M. C. M. (2009). “Scale up of BioGrout: A biological ground reinforcement method.” Proc., 17th Int. Conf. on Soil Mechanics and Geotechnical Engineering, M. Hamza, et al., eds., IOS Press, Amsterdam, Netherlands, 2328–2333.
van Tittelboom, K., Belie, N. D., de Muynck, W., and Verstraete, W. (2010). “Use of bacteria to repair cracks in concrete.” Cement Concr. Res., 40(1), 157–166.
Weil, M. H., DeJong, J. T., Mortensen, B. M., and Martinez, B. C. (2012). “Seismic and resistivity measurements for real-time monitoring of microbially induced calcite precipitation in sand.” ASTM Geotech. Test. J., 35(2), 330–341.
Whiffin, V. S. (2004). “Microbial CaCO3 precipitation for the production of biocement.” Sc.D. thesis, School of Biological Sciences and Biotechnology, Murdoch Univ., Perth, Australia.
Whiffin, V. S., van Paassen, L. A., and Harkes, M. P. (2007). “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. J., 24(5), 417–423.
Yoon, J. S., Germaine, J. T., and Culligan, P. J. (2006). “Visualization of particle behavior within a porous medium: Mecahnisms for particle filtration and retardation during downward transport.” Water Resour. Res., 42(W06417), 1–16.
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Published In
Journal of Geotechnical and Geoenvironmental Engineering
Volume 139 • Issue 4 • April 2013
Pages: 587 - 598
Copyright
© 2013 American Society of Civil Engineers.
History
Received: Apr 12, 2011
Accepted: Jun 4, 2012
Published online: Mar 15, 2013
Published in print: Apr 1, 2013
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