Sessile and Planktonic Microbial Taxonomy of a Methanogenic Cellulolytic Enrichment Reactor Sourced from the Organic Fraction of Municipal Solid Waste
Publication: Journal of Environmental Engineering
Volume 148, Issue 4
Abstract
The bacterial, archaeal, and fungal taxonomies of a semi-batch-fed, lab-scale cellulolytic enrichment reactor inoculated by the organic fraction of municipal solid waste were explored using 16SrRNA and internal transcribed spacer (ITS) sequencing. Biomass samples were partitioned into total, planktonic, and sessile (biofilm) fractions using a centrifugation separation technique developed to quantify sessile biomass growth on cellulose particles during anaerobic digestion. The relative abundances of bacteria and archaea taxa were determined over a 1-day period. The sessile and planktonic biomass fractions showed significantly different relative abundances for 13 taxa, with the sessile biomass having 10 times lower relative abundance of Unclassified Firmicutes, nearly 20 times higher relative abundance of Methanothrix spp., double the relative abundance of Hydrogenispora spp., and 44.3% higher relative abundance of an uncultured Leptospira sp. than the planktonic biomass. This study shows how a relatively simple physical separation method can be employed to add another dimension to taxonomic studies. Better understanding the microbial communities in these systems is a key first step toward achieving performance improvements such as enhanced cellulose solubilization rates and other microbial biotransformations through process, genetic, and/or other manipulations.
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Data Availability Statement
Some or all data, models, or code that support the findings of this study are available from the corresponding author upon reasonable request, including FASTA, OTU profiling, rarefaction curves, alpha and beta diversities, and OTU with taxonomy.
Acknowledgments
Special thanks to Dr. Karen Wawrousek for use of laboratory equipment. This work was supported by the Environmental Research & Education Foundation, Raleigh, North Carolina. No organization was involved in conducting this study or preparing this publication.
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Published In
Journal of Environmental Engineering
Volume 148 • Issue 4 • April 2022
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© 2022 American Society of Civil Engineers.
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Received: Jul 22, 2021
Accepted: Nov 7, 2021
Published online: Jan 27, 2022
Published in print: Apr 1, 2022
Discussion open until: Jun 27, 2022
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