Bioremediation of 2,4-Diaminotoluene in Aqueous Solution Enhanced by Lipopeptide Biosurfactant Production from Bacterial Strains
Publication: Journal of Environmental Engineering
Volume 146, Issue 7
Abstract
This research concentrated on the isolation and preparation of biosurfactant-producing bacterial strains for the degradation of aromatic amine compound 2,4-diaminotoluene (2,4-DAT). Three strains, designated as Isolates 1, 2, and 3 (, , and ), were isolated from soil and used in the degradation studies. Among the three isolates, showed the highest growth rate and degradation capacity, approximately . The highest degrading capability was found in followed by and . The three strains were tested for biosurfactant screening, and the lipopeptide nature of the biosurfactant was identified through characterization studies. The stability of the biosurfactants was also tested at different temperatures and in various pH ranges. The investigation of biosurfactant-mediated remediation was further carried out using the three strains and lipopeptide biosurfactants. Intermediates formed after degradation were analyzed using high-performance liquid chromatography (HPLC), which showed that the compounds were eluted within 3 min. The results showed that biosurfactants raised the degrading capability of these strains for 2,4-DAT by 14.54, 27.15, and .
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Data Availability Statement
Some or all data, models, or code generated or used during the study are available from the corresponding author by request (UV-Vis, FTIR, HPLC, and experimental data).
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Journal of Environmental Engineering
Volume 146 • Issue 7 • July 2020
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©2020 American Society of Civil Engineers.
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Received: Aug 29, 2019
Accepted: Jan 29, 2020
Published online: May 13, 2020
Published in print: Jul 1, 2020
Discussion open until: Oct 13, 2020
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