Enhancing Biological Removal of Methylene Blue from Aqueous Solutions Using Bacillus subtilis Inoculated Rice Straw
Publication: Journal of Environmental Engineering
Volume 145, Issue 1
Abstract
A combination of rice straw and Bacillus subtilis (B. subtilis) was used to remove methylene blue (MB) from wastewater at varied operational conditions. Virtually identical reductions in MB of 58.4, 61.8, 64.6, and 64.6 mg from 1 L solution were achieved for different initial MB concentrations (100, 200, 300, and ) when rice straw was used alone, which were increased to 91.6, 184.6, 275.3, and 368.1 mg after the rice straw was inoculated with B. subtilis. The amounts of total removed MB in the treatment groups were almost 1.5, 3, 4.5, and 6 times of that in the control group without B. subtilis. These results demonstrated that the combination of rice straw and B. subtilis was effective for the removal of MB from aqueous solutions. In the four treatment groups, of the total removed MB, 52.5%, 58.7%, 66.4%, and 76.3% were adsorbed by rice straw and bacterial biomass, respectively, and the rest were removed by microbial biodegradation. The significant improvement in MB removal was mainly because the B. subtilis increased the specific surface area of the straw and produced more functional groups on it. Additionally, microbial biodegradation was another reason for the improvement in MB removal. Furthermore, inoculation of rice straw with B. subtilis represented an efficient means of removing MB from wastewater in an actual project in Minkang Printing and Dyeing Co., Ltd., Sichuan, China. After 4 days of treatment by 2.5 g of rice straw (per liter of wastewater) inoculated with B. subtilis, a 94.5% decolorization rate was achieved, which was higher than that of the currently used biological filter with activated carbon as a filter bed (78.3%).
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Acknowledgments
The authors would like to acknowledge funding support for this study by the National Natural Science Foundation of China (Grant No. 51508043) and the Basic Project of the Science and Technology Department of Sichuan Province (2016JY0015).
References
Almeida, C. A. P., N. A. Debacher, A. J. Downs, L. Cottet, and C. A. D. Mello. 2009. “Removal of methylene blue from colored effluents by adsorption on montmorillonite clay.” J. Colloid Interface Sci. 332 (1): 46–53. https://doi.org/10.1016/j.jcis.2008.12.012.
Bulut, Y., and H. Karaer. 2015. “Adsorption of methylene blue from aqueous solution by crosslinked chitosan/bentonite composite.” J. Dispersion Sci. Technol. 36 (1): 61–67. https://doi.org/10.1080/01932691.2014.888004.
Chander, M., and D. S. Arora. 2014. “Biodegradation of a dye by different white-rot fungi on a novel agro residue based medium.” Lignocellulose 3 (1): 37–50.
Chaudhari, A. U., D. Paul, D. Dhotre, and K. M. Kodam. 2017. “Effective biotransformation and detoxification of anthraquinone dye reactive blue 4 by using aerobic bacterial granules.” Water Res. 122: 603–613. https://doi.org/10.1016/j.watres.2017.06.005.
Cheng, M., G. M. Zeng, D. L. Huang, C. Lai, Z. Wei, N. J. Li, P. Xu, C. Zhang, Y. Zhu, and X. X. He. 2015. “Combined biological removal of methylene blue from aqueous solutions using rice straw and Phanerochaete chrysosporium.” Appl. Microbiol. Biotechnol. 99 (12): 5247–5256. https://doi.org/10.1007/s00253-014-6344-9.
Cui, M. H., D. Cui, L. Gao, A. J. Wang, and H. Y. Cheng. 2016. “Azo dye decolorization in an up-flow bioelectrochemical reactor with domestic wastewater as a cost-effective yet highly efficient electron donor source.” Water Res. 105: 520–526. https://doi.org/10.1016/j.watres.2016.09.027.
Dafale, N., N. N. Rao, S. U. Meshram, and S. R. Wate. 2008. “Decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium-biostimulation and halo tolerance.” Bioresour. Technol. 99 (7): 2552–2558. https://doi.org/10.1016/j.biortech.2007.04.044.
Dharajiya, D., M. Shah, and B. Bajpai. 2016. “Decolorization of simulated textile effluent by Phanerochaete chrysosporium and Aspergillus fumigatus A23.” Nature Env. Pollut. Technol. 15 (3): 825–832.
El-Mekkawi, D. M., F. A. Ibrahim, and M. M. Selim. 2016. “Removal of methylene blue from water using zeolites prepared from Egyptian kaolins collected from different sources.” J. Environ. Chem. Eng. 4 (2): 1417–1422. https://doi.org/10.1016/j.jece.2016.01.007.
Feng, T., and X. Lei. 2013. “Adsorption of Congo Red from aqueous solution onto chitosan/rectorite composite.” Adv. Mater. Res. 690: 438–441. https://doi.org/10.4028/www.scientific.net/AMR.690-693.438.
Feng, Y., H. Zhou, G. Liu, J. Qiao, J. Wang, H. Lu, L. Yang, and Y. Wu. 2012. “Methylene blue adsorption onto swede rape straw (Brassica napus L.) modified by tartaric acid: Equilibrium, kinetic and adsorption mechanisms.” Bioresour. Technol. 125 (12): 138–144. https://doi.org/10.1016/j.biortech.2012.08.128.
Geetha, D., S. Kavitha, and P. S. Ramesh. 2015. “A novel bio-degradable polymer stabilized Ag/TiO2 nanocomposites and their catalytic activity on reduction of methylene blue under natural sun light.” Ecotoxicol. Environ. Saf. 121 (1–2): 126–134. https://doi.org/10.1016/j.ecoenv.2015.04.042.
Huang, D. L., G. M. Zeng, C. L. Feng, S. Hu, X. Y. Jiang, L. Tang, F. F. Su, Y. Zhang, W. Zeng, and H. L. Liu. 2008. “Degradation of lead-contaminated lignocellulosic waste by Phanerochaete chrysosporium and the reduction of lead toxicity.” Environ. Sci. Technol. 42 (13): 4946–4951. https://doi.org/10.1021/es800072c.
Huang, X. Y., H. T. Bu, G. B. Jiang, and M. H. Zeng. 2011. “Cross-linked succinyl chitosan as an adsorbent for the removal of methylene blue from aqueous solution.” Int. J. Biol. Macromol. 49 (4): 643–651. https://doi.org/10.1016/j.ijbiomac.2011.06.023.
Junghanns, C., G. Krauss, and D. Schlosser. 2008. “Potential of aquatic fungi derived from diverse freshwater environments to decolourise synthetic azo and anthraquinone dyes.” Bioresour. Technol. 99 (5): 1225–1235. https://doi.org/10.1016/j.biortech.2007.02.015.
Kalyani, D. C., P. S. Patil, J. P. Jadhav, and S. P. Govindwar. 2008. “Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1.” Bioresour. Technol. 99 (11): 4635–4641. https://doi.org/10.1016/j.biortech.2007.06.058.
Kumar, A., and H. M. Jena. 2016. “Removal of methylene blue and phenol onto prepared activated carbon from Fox nutshell by chemical activation in batch and fixed-bed column.” J. Cleaner Prod. 137: 1246–1259. https://doi.org/10.1016/j.jclepro.2016.07.177.
Liu, W. J., L. Liu, C. Liu, Y. Hao, H. Yang, B. Yuan, and J. H. Jiang. 2016. “Methylene blue enhances the anaerobic decolorization and detoxication of azo dye by Shewanella onediensis MR-1.” Biochem. Eng. J. 110: 115–124. https://doi.org/10.1016/j.bej.2016.02.012.
Nataraj, S. K., K. M. Hosamani, and T. M. Aminabhavi. 2009. “Nanofiltration and reverse osmosis thin film composite membrane module for the removal of dye and salts from the simulated mixtures.” Desalination. 249 (1): 12–17. https://doi.org/10.1016/j.desal.2009.06.008.
Ngah, W. S. W., L. C. Teong, and M. A. K. M. Hanafiah. 2011. “Adsorption of dyes and heavy metal ions by chitosan composites: A review.” Carbohydr. Polym. 83 (4): 1446–1456. https://doi.org/10.1016/j.carbpol.2010.11.004.
Rafatullah, M., O. Sulaiman, R. Hashim, and A. Ahmad. 2010. “Adsorption of methylene blue on low-cost adsorbents: A review.” J. Hazard. Mater. 177 (1–3): 70–80. https://doi.org/10.1016/j.jhazmat.2009.12.047.
Tien, M., and T. K. Kirk. 1988. “Lignin peroxidase of Phanerochaete chrysosporium.” Methods Enzymol. 161 (C): 238–249. https://doi.org/10.1016/0076-6879(88)61025-1.
Wang, J. J., and R. B. Bai. 2016. “Formic acid enhanced effective degradation of methyl orange dye in aqueous solutions under UV-Vis irradiation.” Water Res. 101: 103–113. https://doi.org/10.1016/j.watres.2016.04.044.
Wang, L., J. P. Zhang, and A. Q. Wang. 2011. “Fast removal of methylene blue from aqueous solution by adsorption onto chitosan-g-poly (acrylic acid)/attapulgite composite.” Desalination 266 (1–3): 33–39. https://doi.org/10.1016/j.desal.2010.07.065.
Wu, B. D., R. Yin, G. Y. Zhang, C. Yu, and S. J. Zhang. 2016. “Effects of water chemistry on decolorization in three photochemical processes: Pro and cons of the UV/AA process.” Water Res. 105: 568–574. https://doi.org/10.1016/j.watres.2016.09.037.
Zhang, W. X., H. Yan, H. J. Li, Z. W. Jiang, L. Dong, X. W. Kan, H. Yang, A. M. Li, and R. S. Cheng. 2011. “Removal of dyes from aqueous solutions by straw based adsorbents: Batch and column studies.” Chem. Eng. J. 168 (3): 1120–1127. https://doi.org/10.1016/j.cej.2011.01.094.
Zhu, X. H., Z. F. Zhang, and G. Q. Yan. 2016. “Methylene blue adsorption by novel magnetic chitosan nanoadsorbent.” J. Water Environ. Technol. 14 (2): 96–105. https://doi.org/10.2965/jwet.15-072.
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Published In
Journal of Environmental Engineering
Volume 145 • Issue 1 • January 2019
Copyright
©2018 American Society of Civil Engineers.
History
Received: Mar 26, 2018
Accepted: Jul 9, 2018
Published online: Oct 30, 2018
Published in print: Jan 1, 2019
Discussion open until: Mar 30, 2019
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