SSPRSD Using a Filamentous Fungal Strain Penicillium expansum BS30 Isolated from Wastewater Sludge
Publication: Journal of Environmental Engineering
Volume 136, Issue 7
Abstract
A filamentous fungal strain (Penicillium expansum BS30) isolated from a municipal wastewater treatment plant was used in this study to simultaneously reduce sludge solids, pathogens, and improve the sludge settling and dewaterability [simultaneous solids and pathogens reduction, settling and dewatering (SSPRSD)] in shake flask and 10-L bioreactor experiments. The fungal strain role in the SSPRSD process was evaluated at different temperatures and inoculum (spores) concentrations. The best performance of the process was achieved at incubation temperature of and inoculum concentration of . At these optimal conditions, suspended solids (SS) and volatile SS were degraded and , respectively. The capillary suction time value recorded was lower than that required for sludge dewaterability . The populations of total coliforms and Salmonella (pathogen indicators) were reduced by two and four log cycles, respectively. A study on molecular screening of penicillin biosynthesis gene cluster and toxic organic compounds degrading machinery of the fungal strain was also conducted. It was found that the fungal strain possessed the penicillin-producing gene and toxic organic compounds degrading genes, and therefore may be helpful in degrading these compounds.
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Acknowledgments
Sincere thanks are due to the Natural Sciences and Engineering Research Council of Canada (Grant No. NSERCA 4984, Canada Research Chair) for their financial support. Thanks to the Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT), Québec for providing Ph.D. scholarship to S.B. The views and opinions expressed in this article are those of the writers and should not be construed as the opinions of the U.S. Environmental Protection Agency.
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Published In
Journal of Environmental Engineering
Volume 136 • Issue 7 • July 2010
Pages: 719 - 730
Copyright
© 2010 ASCE.
History
Received: Jun 16, 2009
Accepted: Dec 18, 2009
Published online: Dec 21, 2009
Published in print: Jul 2010
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