Integrated Chemical-Biological Remediation for Polycyclic Aromatic Hydrocarbons Contaminated Soil
Publication: Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 8, Issue 2
Abstract
An integrated slurry-phase remedial system was developed for cleanup of polycyclic aromatic hydrocarbon (PAH) contaminated soil in Singapore. The bench-scale remedial system consisted of a chemical treatment to initiate the degradation of PAHs followed by a biological treatment. The 20% (weight/volume) slurry of PAH contaminated soil was mixed continuously at 180 rpm in the 1 L bioreactor. PAH contaminated soil was synthesized by spiking naphthalene, phenanthrene, and pyrene onto uncontaminated model soil. Fenton’s reagent was added during chemical treatment to initiate degradation of PAHs. The removal efficiency of chemical treatment was 76%. The active PAH-degrading strain of bacteria was isolated from a slurry-phase reactor, where garden soil was used as a source of indigenous microorganisms. The sequencing of 16S rDNA showed that this bacterial strain was close to Pseudomonas beteli (identities=99%, 553 from 556 position). The application of isolated bacteria for bioremediation of PAH contaminated soil resulted in a 96–97% removal of PAHs. It was possible to use the cultivated PAH-degrading microorganisms as an effective inoculum to start up the remediation process. The proposed integrated chemical-biological remedial system was capable of treating PAH contaminated soil efficiently in terms of high-ring PAH degradation, less expensive process, and short treatment time required.
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Published In
Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 8 • Issue 2 • April 2004
Pages: 79 - 83
Copyright
Copyright © 2004 American Society of Civil Engineers.
History
Received: Mar 13, 2003
Accepted: Mar 13, 2003
Published online: Mar 15, 2004
Published in print: Apr 2004
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