Microbial Dechlorination of Hexachlorobenzene by Untamed Sediment Microorganisms in Taiwan
Publication: Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 8, Issue 2
Abstract
To test the inherent hexachlorobenzene (HCB) dechlorination ability of indigenous consortia in Taiwan, microbes from four non-HCB-contaminated river sediments were collected and directly introduced to dechlorination experiments without any acclimation and enrichment. In natural sediment-extracted waters, when amended with 5 g/L of yeast extract, all four sets of sediment microorganisms initiated HCB dechlorination after 60 days. Without yeast extract, there were only two sets of microbes-dechlorinated HCB during a 150-day incubation period. On the other hand, in the artificial synthetic media amended with 5 g/L of yeast extract, all microbes from four sediments showed HCB-dechlorinating activity after 45–60 days of lag phases and completed dechlorination in latter 30 days. In all HCB-dechlorinated sets, dechlorination was accompanied by significant methane generation and the drop of oxidation-reduction potential (ORP). The results indicated that there were HCB-dechlorinating consortia grouped by the indigenous microorganisms in the river sediments of Taiwan, even though these sediments had not been contaminated by HCB before. In addition, the dechlorination could occur when the environmental conditions were suitable for the dechlorinating consortia to be enriched, and then the ability for HCB decomposition was built up.
Get full access to this article
View all available purchase options and get full access to this article.
References
Bestteti, G., Galli, E., Leoni, B., Pelizzoni, F., and Sello, G.(1992). “Regioselective hydroxylation of chlorobenzenes and chlorophenols by Pseudomonas putida.” Appl. Environ. Microbiol., 37, 260–263.
Chang, B. V., Chen, I. M., Yuan, S. Y., and Wang, Y. S.(1997). “Reductive dechlorination of hexachlorobenzene by an anaerobic mixed culture.” Water, Air, Soil Pollut., 100, 25.
Chang, B. V., Su, C. J., and Yuan, S. Y.(1998). “Microbial hexachlorobenzene dechlorination under three reducing conditions.” Chemosphere, 36(13), 2721–2730.
Chen, I. M., Chang, F. C., Chang, B. V., and Wang, Y. S.(2000). “Specificity of the microbial activities in the reductive dechlorination of chlorinated benzenes.” Water Environ. Res., 72(6), 675–679.
Chen, I. M., Chang, F. C., and Wang, Y. S. (1997). “Reductive dechlorination of hexachlorobenzene and polychlorinated biphenyls in anaerobic sediments from tropical rivers with enrichment.” Proc., 17th Symp. on Chlorinated Dioxins and Related Compounds, Indianapolis, August 25–29; Organohalogen Compounds, 33, 189–192.
Chen, I. M., Chang, F. C., and Wang, Y. S.(2001). “Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms.” Chemosphere, 45(2), 223–229.
Chen, I. M., Chang, B. V., Yuan, S. Y., and Wang, Y. S.(2002). “Reductive dechlorination of hexachlorobenzene under various additions.” Water, Air, Soil Pollut., 139, 61–74.
De Bont, J. A. M., Vorage, M. J. A. W., Hartmans, S., and Van Den Tweel, W. T. J.(1986). “Microbial degradation of 1,3-dichlorobenzene.” Appl. Environ. Microbiol., 52, 677–680.
Haigler, B. E., Pettigrew, C. A., and Spain, J. C.(1992). “Biodegradation of mixtures of substituted benzenes by Psudomonas sp. Strain JS150.” Appl. Environ. Microbiol., 58, 348–353.
Holliger, C., Schraa, G., Stams, A. J. M., and Zehnder, A. J. B.(1992). “Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene.” Appl. Environ. Microbiol., 58, 1636–1644.
Langlolis, C., and Sloterdijk, H.(1989). “Contamination of Lake Saint-Pierre (St. Lawrence River) by organic and inorganic pollutants.” Rev. Sci. Eau., 2, 659–670.
Middeldorp, P. J. M., De Wolf, J., Zehnder, A. J. B., and Schraa, G.(1997). “Enrichment and properties of a 1,2,4-trichlorobenzene-dechlorinating methanogenic microbial consortium.” Appl. Environ. Microbiol., 63(4), 1225–1229.
Nowak, J., Kirsch, N. H., Hegemann, W., and Stan, H. J.(1996). “Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment.” Appl. Microbiol. Biotechnol., 45, 700–709.
Pearson, C. R. (1982). “Halogenated aromatics.” The handbook of environmental chemistry, O. Huntzinger, ed., Vol. 3, Part B, Springer, New York.
Prytula, M. T., and Pavlostathis, S. G.(1996). “Effect of contaminant and organic matter bioavailability on the microbial dehalogenation of sediment-bound chlorobenzenes.” Water Resour., 30(11), 2669–2680.
Rosenbrock, P., Martens, R., Buscot, F., and Munch, J. C.(1997). “Initiation of hexachlorobenzene dechlorination in three different soils under artificially induced anaerobic conditions.” Appl. Microbiol. Biotechnol., 48, 115–120.
Van Hoof, P. L., and Jafvert, C. T.(1996). “Reductive dechlorination of chlorobenzenes in surfactant-amended sediment slurries.” Environ. Toxicol. Chem., 15(11), 1914–1924.
Information & Authors
Information
Published In
Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 8 • Issue 2 • April 2004
Pages: 73 - 78
Copyright
Copyright © 2004 American Society of Civil Engineers.
History
Received: Mar 11, 2003
Accepted: Mar 11, 2003
Published online: Mar 15, 2004
Published in print: Apr 2004
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.