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Apr 26, 2012
Novel, Rapid Molecular-Based Technique for Detecting Contamination in Drinking Water Distribution Systems
Authors: Stacia L. Thompson, Ph.D. [email protected], and Jeanne M. VanBriesen, Ph.D. [email protected]Author Affiliations
Publication: World Environmental and Water Resources Congress 2009: Great Rivers
Abstract
Drinking water distribution systems are vulnerable to biological contamination via many potential intrusion locations; thus, a comprehensive water quality indicator is critical. While previous research has focused on specific pathogen detection using molecular methods, this study seeks to confirm that bacterial indicator classes, specifically, Alpha-, Beta-, and Gammaproteobacteria, can be used as a more comprehensive indicator. The system population exhibits temporal variability in response to changes, such as temperature and disinfectant concentration, but this seasonal bacterial diversity study confirms that these indicator classes are a consistent presence in drinking water. Bacterial class-specific primers were designed to target Alpha-, Beta-, and Gammaproteobacteria, and utilized in Quantitative Polymerase Chain Reaction (QPCR) assays to detect surrogate contaminants. Preliminary results indicate that this QPCR-based approach is a faster, more comprehensive indicator than the culture-based regulatory technique.
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© 2009 American Society of Civil Engineers.
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Published online: Apr 26, 2012
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119 Porter Hall, Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA 15213. E-mail: [email protected]
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119 Porter Hall, Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA 15213. E-mail: [email protected]
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