Technical Papers
Jan 28, 2022

Performance Enhancement and Remediation of Microcracks in Cement Mortar by Doping Calcite-Precipitating Microorganisms

Publication: Journal of Materials in Civil Engineering
Volume 34, Issue 4

Abstract

Concrete is prone to microcracks owing to its brittle nature and durability-related issues when subjected to loading and various environmental conditions. This necessitates the need for an effective crack-healing method combined with reliable techniques to monitor the healing. This paper reports experiments conducted on endospore-forming alkaliphilic calcite-precipitating bacterium Bacillus megaterium MTCC 8510 and its efficacy at enhancing the performance and crack remediation in cement mortar. The capacity and the presence of calcite precipitation were confirmed using X-ray diffraction and field emission scanning electron microscopy. The effects of the direct addition of bacteria to cement mortar on its compressive strength, chloride diffusivity, and water permeability were observed. Apart from the direct addition of bacteria, the crack-healing capacity was also observed by spraying the bacterial solution directly on the cracks every day on a coir-reinforced cement mortar specimen. The results of the compressive strength test, rapid chloride penetration test (RCPT), and water permeability tests showed improved strength, reduced chloride diffusivity, and diminished water permeability with bacteria directly added cement mortar specimens. The healing of cracks inside the mortar specimens was confirmed by ultrasonic pulse velocity testing. The crack area was found using ImageJ software, which showed 94.73% healing in the surface crack area by direct spraying on the surface. It was found that the bacterial strain selected was efficient in terms of improving the performance characteristics and healing of microcracks.

Get full access to this article

View all available purchase options and get full access to this article.

Data Availability Statement

All data, models, and code generated or used during the study appear in the published article.

References

Achal, V., A. Mukherjee, D. Kumari, and Q. Zhang. 2015. “Biomineralization for sustainable construction: A review of processes and applications.” Earth Sci. Rev. 148 (Sep): 1–17. https://doi.org/10.1016/j.earscirev.2015.05.008.
Ahmad, W., S. H. Farooq, M. Usman, M. Khan, A. Ahmad, F. Aslam, R. A. Yousef, H. A. Abduljabbar, and M. Sufian. 2020. “Effect of coconut fiber length and content on properties of high strength concrete.” Materials 13 (5): 1075. https://doi.org/10.3390/ma13051075.
Almusallam, A. A., F. M. Khan, S. U. Dulaijan, and O. S. B. Al-Amoudi. 2003. “Effectiveness of surface coatings in improving concrete durability.” Cem. Concr. Compos. 25 (4–5): 473–481. https://doi.org/10.1016/S0958-9465(02)00087-2.
Anbu, P., C. H. Kang, Y. J. Shin, and J. S. So. 2016. “Formations of calcium carbonate minerals by bacteria and its multiple applications.” Springerplus 5 (1): 1–26. https://doi.org/10.1186/s40064-016-1869-2.
Arsyad, M., I. Wardana, and Y. S. Irawan. 2015. “The morphology of coconut fiber surface under chemical treatment.” Matéria (Rio J.) 20 (1): 169–177. https://doi.org/10.1590/S1517-707620150001.0017.
ASTM. 2019. Standard test method for electrical indication of concrete’s ability to resist chloride ion penetration. ASTM C1202. West Conshohocken, PA: ASTM.
BIS (Bureau of Indian Standards). 1988. Methods of physical tests for hydraulic cement. IS 4031 (Part 6). New Delhi, India: BIS.
BIS (Bureau of Indian Standards). 1991. Specification for standard sand for testing of cement. IS 650: 1991(R2008). New Delhi, India: BIS.
BIS (Bureau of Indian Standards). 2013. Ordinary portland cement, 53 grade: Specification. IS 12269:2013. New Delhi, India: BIS.
BIS (Bureau of Indian Standards). 2016. Methods of test for permeability of cement mortar and concrete (Seventh revision). IS 3085:1965. New Delhi, India: BIS.
Chunxiang, Q., W. Jianyun, W. Ruixing, and C. Liang. 2009. “Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii.” Mater. Sci. Eng., C 29 (4): 1273–1280. https://doi.org/10.1016/j.msec.2008.10.025.
Diamanti, M. V., A. Brenna, F. Bolzoni, T. Berra, T. Pastore, and M. Ormellese. 2013. “Effect of polymer modified cementitious coatings on water and chloride permeability in concrete.” Constr. Build. Mater. 49: 720–728. https://doi.org/10.1016/j.conbuildmat.2013.08.050.
Dikshit, R., A. Jain, A. Dey, and A. Kumar. 2020. “Microbially induced calcite precipitation using Bacillus velezensis with guar gum.” PLoS One 15 (8): e0236745. https://doi.org/10.1371/journal.pone.0236745.
Edison, A. J., V. Poornima, and R. Venkatasubramani. 2015. “Influence of bacteria on strength and self-healing characteristics of fly ash concrete.” Int. J. Appl. Eng. Res. 10 (19): 13929–13933.
Ersan, Y. C., N. Boon, and N. De Belie. 2015. “Microbial self-healing concrete: Denitrification as an enhanced and environment-friendly approach.” In Proc., 5th Int. Conf. on Self-Healing Materials (ICSHM 2015). Chapel Hill, NC: Univ. of North Carolina at Chapel Hill.
Esaker, M., O. Hamza, A. Souid, and D. Elliott. 2021. “Self-healing of bio-cementitious mortar incubated within neutral and acidic soil.” Mater. Struct. 54 (2): 1–16. https://doi.org/10.1617/s11527-021-01690-1.
Ferrara, L., V. Krelani, and M. Carsana. 2014. “A ‘fracture testing’ based approach to assess crack healing of concrete with and without crystalline admixtures.” Constr. Build. Mater. 68 (Oct): 535–551. https://doi.org/10.1016/j.conbuildmat.2014.07.008.
Gowtham, D., V. Poornima, and R. Venkatasubramani. 2015. “Effect of bacillus subtilis on biocrete using silica fume.” Int. J. Appl. Eng. Res. 10 (1): 14021–14025.
Hammad, I. A., F. N. Talkhan, and A. E. Zoheir. 2013. “Urease activity and induction of calcium carbonate precipitation by Sporosarcina pasteurii NCIMB 8841.” J. Appl. Sci. Res. 9 (3): 1525–1533.
Jiang, L., G. Jia, C. Jiang, and Z. Li. 2020a. “Sugar-coated expanded perlite as a bacterial carrier for crack-healing concrete applications.” Constr. Build. Mater. 232 (Jan): 117222. https://doi.org/10.1016/j.conbuildmat.2019.117222.
Jiang, L., G. Jia, Y. Wang, and Z. Li. 2020b. “Optimization of sporulation and germination conditions of functional bacteria for concrete crack-healing and evaluation of their repair capacity.” ACS Appl. Mater. Interfaces 12 (9): 10938–10948. https://doi.org/10.1021/acsami.9b21465.
Jonkers, H. M. 2011. “Bacteria-based self-healing concrete.” Heron 56 (1–2): 1–12.
Jonkers, H. M., A. Thijssen, G. Muyzer, O. Copuroglu, and E. Schlangen. 2010. “Application of bacteria as self-healing agent for the development of sustainable concrete.” Ecol. Eng. 36 (2): 230–235. https://doi.org/10.1016/j.ecoleng.2008.12.036.
Khaliq, W., and M. B. Ehsan. 2016. “Crack healing in concrete using various bio influenced self-healing techniques.” Constr. Build. Mater. 102 (Part 1): 349–357. https://doi.org/10.1016/j.conbuildmat.2015.11.006.
Krajewska, B. 2018. “Urease-aided calcium carbonate mineralization for engineering applications: A review.” J. Adv. Res. 13 (Sep): 59–67. https://doi.org/10.1016/j.jare.2017.10.009.
Krishna, N. K., M. Prasanth, R. Gowtham, S. Karthic, and K.M. Mini. 2018. “Enhancement of properties of concrete using natural fibers.” Mater. Today: Proc. 5 (11): 23816–23823. https://doi.org/10.1016/j.matpr.2018.10.173.
Krishnapriya, S., and D. V. Babu. 2015. “Isolation and identification of bacteria to improve the strength of concrete.” Microbiol. Res. 174 (May): 48–55. https://doi.org/10.1016/j.micres.2015.03.009.
Kuang, Y. C., and J. P. Ou. 2008. “Passive smart self-repairing concrete beams by using shape memory alloy wires and fibers containing adhesives.” J. Central South Univ. Technol. 15 (3): 411–417. https://doi.org/10.1007/s11771-008-0077-9.
Kumar, M. P., K. M. Mini, and M. Rangarajan. 2018. “Ultrafine GGBS and calcium nitrate as concrete admixtures for improved mechanical properties and corrosion resistance.” Constr. Build. Mater. 182 (Sep): 249–257. https://doi.org/10.1016/j.conbuildmat.2018.06.096.
Li, Z., L. Wang, and X. Wang. 2006. “Flexural characteristics of coir fiber reinforced cementitious composites.” Fibers Polym. 7 (3): 286–294. https://doi.org/10.1007/BF02875686.
Liu, I. S., D. J. Oehlers, and R. Seracino. 2006. “Tests on the ductility of reinforced concrete beams retrofitted with FRP and steel near-surface mounted plates.” J. Compos. Constr. 10 (2): 106–114. https://doi.org/10.1061/(ASCE)1090-0268(2006)10:2(106).
Manikandan, A. T., and A. Padmavathi. 2015. “An experimental investigation on improvement of concrete serviceability by using bacterial mineral precipitation.” Int. J. Res. Sci. Innovation 2 (3): 48.
Mini, K. M., R. J. Alapatt, A. E. David, A. Radhakrishnan, M. M. Cyriac, and R. Ramakrishnan. 2014. “Experimental study on strengthening of RC beam using glass fibre reinforced composite.” Struct. Eng. Mech. 50 (3): 275–286. https://doi.org/10.12989/sem.2014.50.3.275.
Padanattil, A., J. Karingamanna, and K. M. Mini. 2017. “Novel hybrid composites based on glass and sisal fiber for retrofitting of reinforced concrete structures.” Constr. Build. Mater. 133 (Feb): 146–153. https://doi.org/10.1016/j.conbuildmat.2016.12.045.
Rameshkumar, V., S. Prabhath Ranjan Kumar, V. Poornima, R. Venkatasubramani, and V. Sreevidya. 2020. “Improvements in mechanical and durability parameters of bio-engineered concrete with metakaolin as a partial substitute for cement.” Eur. J. Environ. Civ. Eng. 1–14. https://doi.org/10.1080/19648189.2020.1767696.
Rao, M., V. S. Reddy, M. Hafsa, P. Veena, and P. Anusha. 2013. “Bioengineered concrete—A sustainable self-healing construction material.” Res. J. Eng. Sci. 2 (6): 45–51.
Reddy, S., and J. Kumar. 2013. “Studies on permeability of self-healing built-in bacteria concrete.” Int. J. Recent Technol. Eng. 1 (6): 119–125.
Singh, H., and R. Gupta. 2020. “Cellulose fiber as bacteria-carrier in mortar: Self-healing quantification using UPV.” J. Build. Eng. 28 (Mar): 101090. https://doi.org/10.1016/j.jobe.2019.101090.
Soda, P. R. K., V. Poornima, V. Rameshkumar, and R. Venkatasubramani. 2017. “Influence of ureolytic bacteria in improving performance characteristics of concrete.” Ecol. Environ. Conserv. 23: S57–S63.
Stanaszek-Tomal, E. 2020. “Bacterial concrete as a sustainable building material?” Sustainability 12 (2): 696. https://doi.org/10.3390/su12020696.
Sumathi, A., G. Murali, D. Gowdhaman, M. Amran, R. Fediuk, N. I. Vatin, R. Deeba Laxme, and T. S. Gowsika. 2020. “Development of bacterium for crack healing and improving properties of concrete under wet–dry and full-wet curing.” Sustainability 12 (24): 10346. https://doi.org/10.3390/su122410346.
Talaiekhozani, A., and M. Z. Abd Majid. 2014. “A review of self-healing concrete research development.” J. Environ. Treat. Tech. 2 (1): 1–11.
Tayebani, B., and D. Mostofinejad. 2019. “Self-healing bacterial mortar with improved chloride permeability and electrical resistance.” Constr. Build. Mater. 208 (May): 75–86. https://doi.org/10.1016/j.conbuildmat.2019.02.172.
Wang, J., A. Mignon, D. Snoeck, V. Wiktor, S. Van Vliergerghe, N. Boon, and N. De Belie. 2015. “Application of modified-alginate encapsulated carbonate producing bacteria in concrete: A promising strategy for crack self-healing.” Front. Microbiol. 6: 1088. https://doi.org/10.3389/fmicb.2015.01088.
Wang, J., K. Van Tittelboom, N. De Belie, and W. Verstraete. 2012. “Use of silica gel or polyurethane immobilized bacteria for self-healing concrete.” Constr. Build. Mater. 26 (1): 532–540. https://doi.org/10.1016/j.conbuildmat.2011.06.054.
Wang, J. Y., H. Soens, W. Verstraete, and N. De Belie. 2014. “Self-healing concrete by use of microencapsulated bacterial spores.” Cem. Concr. Res. 56 (Feb): 139–152. https://doi.org/10.1016/j.cemconres.2013.11.009.
Wiktor, V., and H. M. Jonkers. 2011. “Quantification of crack-healing in novel bacteria-based self-healing concrete.” Cem. Concr. Compos. 33 (7): 763–770. https://doi.org/10.1016/j.cemconcomp.2011.03.012.
Zhou, J., F. Bi, Z. Wang, and J. Zhang. 2016. “Experimental investigation of size effect on mechanical properties of carbon fiber reinforced polymer (CFRP) confined concrete circular specimens.” Constr. Build. Mater. 127 (Nov): 643–652. https://doi.org/10.1016/j.conbuildmat.2016.10.039.

Information & Authors

Information

Published In

Go to Journal of Materials in Civil Engineering
Journal of Materials in Civil Engineering
Volume 34Issue 4April 2022

History

Received: Nov 24, 2020
Accepted: Sep 3, 2021
Published online: Jan 28, 2022
Published in print: Apr 1, 2022
Discussion open until: Jun 28, 2022

Permissions

Request permissions for this article.

Authors

Affiliations

Prabhath Ranjan Kumar Soda
Research Scholar, Dept. of Civil Engineering, Amrita School of Engineering, Coimbatore, Tamil Nadu 641112, India; Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu 641112, India.
Professor, Dept. of Civil Engineering, Amrita School of Engineering, Coimbatore, Tamil Nadu 641112, India; Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu 641112, India (corresponding author). ORCID: https://orcid.org/0000-0002-5601-2209. Email: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

  • A Binary Microorganism Self-Healing Agent for Concrete Cracks Comprising Bacillus pasteurii and Saccharomyces cerevisiae, Journal of Materials in Civil Engineering, 10.1061/JMCEE7.MTENG-16409, 36, 3, (2024).
  • Experimental and statistical investigation on strength and microcracks remediation in cement mortar using expanded vermiculite as a bacterial carrier, Journal of Building Engineering, 10.1016/j.jobe.2022.105567, 63, (105567), (2023).
  • Statistical and experimental investigation on self-healing of microcracks in cement mortar by encapsulation of calcite precipitating bacteria into expanded perlite, Construction and Building Materials, 10.1016/j.conbuildmat.2022.127985, 342, (127985), (2022).
  • A Critical Review on Assessment of Self Healing Performance of Bioconcrete, KSCE Journal of Civil Engineering, 10.1007/s12205-022-2161-3, 27, 2, (740-750), (2022).

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share