Effect of Calcium Organic Additives on the Self-Healing of Concrete Microcracks in the Presence of a New Isolate Bacillus sp. BY1
Publication: Journal of Materials in Civil Engineering
Volume 31, Issue 10
Abstract
Calcium carbonate–based biominerals are considered self-healing materials in concrete. The morphology and mineralogy of biominerals depend highly on the self-healing efficiency of concrete cracks. This study examined the morphology and mineralogy of biominerals with a new isolate, Bacillus sp. BY1, and various organic calcium compounds (i.e., calcium formate, calcium acetate, and calcium lactate). In addition, compressive strength and crack self-healing efficiency were investigated under various other conditions. Biominerals formed in the presence of calcium formate and calcium lactate were mostly calcite (ca. 95% by weight) and contained more rhombohedral faces, whereas calcium acetate induced spherulite-shaped biominerals with a smaller fraction of calcite (ca. 61.5% by weight). With the addition of bacteria and organic calcium compounds, the compressive strengths decreased and increased, respectively; however, the loss of strength by the bacteria was compensated when both bacteria and organic calcium compounds were added together. Self-healing of cracks did not occur by the addition of bacteria alone and was more prominent when calcium lactate was used as a biomineral precursor.
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Acknowledgments
This work was supported by the Korea Environment Industry & Technology Institute through the Subsurface Environment Management Project (2018002450002) and partially by the Hankuk University of Foreign Studies Research Fund. The authors would like to thank the Institute of Engineering Research at Seoul National University for its technical assistance.
References
Bang, S. S., J. K. Galinat, and V. Ramakrishnan. 2001. “Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii.” Enzyme Microb. Technol. 28 (4–5): 404–409. https://doi.org/10.1016/S0141-0229(00)00348-3.
Becker, A., W. Becker, J. C. Marxen, and M. Epple. 2003. “In-vitro crystallization of calcium carbonate in the presence of biological additives—Comparison of the ammonium carbonate method with double-diffusion techniques.” J. Inorg. Gen. Chem. 629 (12–13): 2305–2311. https://doi.org/10.1002/zaac.200300229.
Boquet, E., A. Boronat, and A. Ramos-Cormenzana. 1973. “Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon.” Nature 246 (5434): 527–529. https://doi.org/10.1038/246527a0.
Braissant, O., G. Cailleau, C. Dupraz, and E. P. Verrecchia. 2003. “Bacterially induced mineralization of calcium carbonate in terrestrial environments: The role of exopolysaccharides and amino acids.” J. Sediment. Res. 73 (3): 485–490. https://doi.org/10.1306/111302730485.
Castanier, S., G. Le Métayer-Levrel, and J. P. Perthuisot. 1999. “Ca-carbonates precipitation and limestone genesis—The microbiogeologist point of view.” Sediment. Geol. 126 (1–4): 9–23. https://doi.org/10.1016/S0037-0738(99)00028-7.
Cheung, J., A. Jeknavorian, L. Roberts, and D. Silva. 2011. “Impact of admixtures on the hydration kinetics of Portland cement.” Cem. Concr. Res. 41 (12): 1289–1309. https://doi.org/10.1016/j.cemconres.2011.03.005.
De Muynck, W., N. De Belie, and W. Verstraete. 2010. “Microbial carbonate precipitation in construction materials: A review.” Ecol. Eng. 36 (2): 118–136. https://doi.org/10.1016/j.ecoleng.2009.02.006.
De Muynck, W., D. Debrouwer, N. De Belie, and W. Verstraete. 2008. “Bacterial carbonate precipitation improves the durability of cementitious materials.” Cem. Concr. Res. 38 (7): 1005–1014. https://doi.org/10.1016/j.cemconres.2008.03.005.
Dick, J., W. De Windt, B. De Graef, H. Saveyn, P. Van Der Meeren, N. De Belie, and W. Verstraete. 2006. “Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species.” Biodegradation 17 (4): 357–367. https://doi.org/10.1007/s10532-005-9006-x.
Edvardsen, C. 1999. “Water permeability and autogenous healing of cracks in concrete.” ACI Mater. J. 96 (4): 448–454.
Ferrer, M. R., J. Quevedo-Sarmiento, M. A. Rivadeneyra, V. Bejar, R. Delgado, and A. Ramos-Cormenzana. 1988. “Calcium carbonate precipitation by two groups of moderately halophilic microorganisms at different temperatures and salt concentrations.” Curr. Microbiol. 17 (4): 221–227. https://doi.org/10.1007/BF01589456.
Gollapudi, U. K., C. L. Knutson, S. S. Bang, and M. R. Islam. 1995. “A new method for controlling leaching through permeable channels.” Chemosphere 30 (4): 695–705. https://doi.org/10.1016/0045-6535(94)00435-W.
Hammes, F., and W. Verstraete. 2002. “Key roles of pH and calcium metabolism in microbial carbonate precipitation.” Rev. Environ. Sci. Biotechnol. 1 (1): 3–7. https://doi.org/10.1023/A:1015135629155.
Jonkers, H. M., and E. Schlangen. 2008. “Development of a bacteria-based self healing concrete.” In Proc., Int. FIB Symp., 425–430. London: Taylor & Francis.
Jonkers, H. M., and E. Schlangen. 2009. “A two component bacteria-based self-healing concrete.” In Vol. 8 of Proc., 2nd Int. Conf. on Concrete Repair, Rehabilitation and Retrofitting, ICCRRR-2. Boca Raton, FL: CRC Press.
Jonkers, H. M., A. Thijssen, G. Muyzer, O. Copuroglu, and E. Schlangen. 2010. “Application of bacteria as self-healing agent for the development of sustainable concrete.” Ecol. Eng. 36 (2): 230–235. https://doi.org/10.1016/j.ecoleng.2008.12.036.
Joshi, S., S. Goyal, A. Mukherjee, and M. S. Reddy. 2017. “Microbial healing of cracks in concrete: A review.” J. Ind. Microbiol. Biotechnol. 44 (11): 1511–1525. https://doi.org/10.1007/s10295-017-1978-0.
Kaur, N., M. S. Reddy, and A. Mukherjee. 2013. “Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites.” J. Microbiol. Biotechnol. 23 (5): 707–714. https://doi.org/10.4014/jmb.1212.11087.
Kawaguchi, T., and A. W. Decho. 2002. “A laboratory investigation of cyanobacterial extracellular polymeric secretions (EPS) in influencing polymorphism.” J. Cryst. Growth 240 (1): 230–235. https://doi.org/10.1016/S0022-0248(02)00918-1.
Korean Standard Association. 2011. Methods of testing cements—Determination of strength. KS L ISO 679. Seoul: Korean Standard Association.
Korean Standard Association. 2012. Testing method for mechanical mixing of hydraulic cement pastes and mortars of plastic consistency. KS L 5109. Seoul: Korean Standard Association.
Korean Standard Association. 2013. Portland cement. KS L 5201. Seoul: Korean Standard Association.
Korean Standard Association. 2014. Standard test method of making and curing concrete specimens. KS F 2403. Seoul: Korean Standard Association.
Li, W., W. S. Chen, P. P. Zhou, S. L. Zhu, and L. J. Yu. 2013. “Influence of initial calcium ion concentration on the precipitation and crystal morphology of calcium carbonate induced by bacterial carbonic anhydrase.” Chem. Eng. J. 218: 65–72. https://doi.org/10.1016/j.cej.2012.12.034.
Lian, B., Q. Hu, J. Chen, J. Ji, and H. H. Teng. 2006. “Carbonate biomineralization induced by soil bacterium Bacillus megaterium.” Geochim. Cosmochim. Acta 70 (22): 5522–5535. https://doi.org/10.1016/j.gca.2006.08.044.
Meldrum, F. C., and S. T. Hyde. 2001. “Morphological influence of magnesium and organic additives on the precipitation of calcite.” J. Cryst. Growth 231 (4): 544–558. https://doi.org/10.1016/S0022-0248(01)01519-6.
Morita, R. Y. 1980. “Calcite precipitation by marine bacteria.” Geomicrobiol. J. 2 (1): 63–82. https://doi.org/10.1080/01490458009377751.
Qiu, J., and E. H. Yang. 2015. “Effects of microbial carbonate precipitation on transport properties of fiber cement composites.” J. Mater. Civ. Eng. 28 (5): 04015204. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001500.
Ramachandran, S. K., V. Ramakrishnan, and S. S. Bang. 2001. “Remediation of concrete using micro-organisms.” ACI Mater. J. 98 (1): 3–9.
Rivadeneyra, M. A., R. Delgado, A. del Moral, M. R. Ferrer, and A. Ramos-Cormenzana. 1994. “Precipitation of calcium carbonate by Vibrio spp. from an inland saltern.” FEMS Microbiol. Ecol. 13 (3): 197–204. https://doi.org/10.1111/j.1574-6941.1994.tb00066.x.
Rivadeneyra Torres, A., M. V. Martinez-Toledo, A. Gonzalez-Martinez, J. Gonzalez-Lopez, D. Martín-Ramos, and M. A. Rivadeneyra. 2013. “Precipitation of carbonates by bacteria isolated from wastewater samples collected in a conventional wastewater treatment plant.” Int. J. Environ. Sci. Technol. 10 (1): 141–150. https://doi.org/10.1007/s13762-012-0084-0.
Rodriguez-Navarro, C., M. Rodriguez-Gallego, K. B. Chekroun, and M. T. Gonzalez-Munoz. 2003. “Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization.” Appl. Environ. Microbiol. 69 (4): 2182–2193. https://doi.org/10.1128/AEM.69.4.2182-2193.2003.
Rosskopf, P. A., F. J. Linton, and R. B. Peppler. 1975. “Effect of various accelerating chemical admixtures on setting and strength development of concrete.” J. Test. Eval. 3 (4): 322–330. https://doi.org/10.1520/JTE10662J.
Setlow, P. 2006. “Spores of Bacillus subtilis: Their resistance to and killing by radiation, heat and chemicals.” J. Appl. Microbiol. 101 (3): 514–525. https://doi.org/10.1111/j.1365-2672.2005.02736.x.
Singh, N. B., S. Prabha, and A. K. Singh. 1986. “Effect of lactic acid on the hydration of portland cement.” Cem. Concr. Res. 16 (4): 545–553. https://doi.org/10.1016/0008-8846(86)90092-X.
Skalny, J., and J. Maycock. 1975. “Mechanisms of acceleration by calcium chloride: A review.” J. Test. Eval. 3 (4): 303–311. https://doi.org/10.1520/JTE10660J.
Stepkowska, E. T., J. L. Pérez-Rodríguez, M. J. Sayagués, and J. M. Martínez-Blanes. 2003. “Calcite, vaterite and aragonite forming on cement hydration from liquid and gaseous phase.” J. Therm. Anal. Calorim. 73 (1): 247–269. https://doi.org/10.1023/A:1025158213560.
Stocks-Fischer, S., J. K. Galinat, and S. S. Bang. 1999. “Microbiological precipitation of .” Soil Biol. Biochem. 31 (11): 1563–1571. https://doi.org/10.1016/S0038-0717(99)00082-6.
Van Tittelboom, K., N. De Belie, W. De Muynck, and W. Verstraete. 2010. “Use of bacteria to repair cracks in concrete.” Cem. Concr. Res. 40 (1): 157–166. https://doi.org/10.1016/j.cemconres.2009.08.025.
Wang, K., D. C. Jansen, S. P. Shah, and A. F. Karr. 1997. “Permeability study of cracked concrete.” Cem. Concr. Res. 27 (3): 381–393. https://doi.org/10.1016/S0008-8846(97)00031-8.
Wiktor, V., and H. M. Jonkers. 2011. “Quantification of crack-healing in novel bacteria-based self-healing concrete.” Cem. Concr. Compos. 33 (7): 763–770. https://doi.org/10.1016/j.cemconcomp.2011.03.012.
Wu, Q. S., D. M. Sun, H. J. Liu, and Y. P. Ding. 2004. “Abnormal polymorph conversion of calcium carbonate and nano-self-assembly of vaterite by a supported liquid membrane system.” Cryst. Growth Des. 4 (4): 717–720. https://doi.org/10.1021/cg034247u.
Yi, S. T., T. Y. Hyun, and J. K. Kim. 2011. “The effects of hydraulic pressure and crack width on water permeability of penetration crack-induced concrete.” Constr. Build. Mater. 25 (5): 2576–2583. https://doi.org/10.1016/j.conbuildmat.2010.11.107.
Yu, J., M. Lei, B. Cheng, and X. Zhao. 2004. “Facile preparation of calcium carbonate particles with unusual morphologies by precipitation reaction.” J. Cryst. Growth 261 (4): 566–570. https://doi.org/10.1016/j.jcrysgro.2003.09.035.
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©2019 American Society of Civil Engineers.
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Received: Apr 9, 2018
Accepted: Nov 30, 2018
Published online: Jul 26, 2019
Published in print: Oct 1, 2019
Discussion open until: Dec 26, 2019
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