Phenolic Precipitates from Soybean Peroxidase–Catalyzed Wastewater Treatment: Concentrated Waste Serves to Concentrate Its Progenitor
Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 20, Issue 2
Abstract
The use of Triton X-100 (Triton) significantly improves enzyme economy for the removal of phenol from wastewater with crude soybean peroxidase (SBP, Enzyme Commission Number 1.11.1.7), a polymerization process that forms (poly) phenolic precipitates. The observation of SBP elution from phenolic precipitates in the presence of Triton was considered a competitive adsorption displacement phenomenon in order to explain the protection mechanism (Triton effect). Both SBP and Triton adsorption on the precipitates were characterized by Langmuir adsorption isotherms as single-adsorbate systems. As a binary-adsorbate system, the adsorption of Triton gradually reversed the adsorbed SBP back into the aqueous phase. The sigmoid elution curve was consistent with an orogenic displacement mechanism. A quantitative relationship between SBP elution as a function of Triton and precipitates concentrations was established by adapting the curves to a logistic function. The concept of using phenolic precipitates as an affinity matrix for concentrating SBP from dilute solution was proven and the feasibility of developing a related process was validated in both single-batch and consecutive cycles of operation. The process has the potential for producing low-cost enzyme concentrate, thereby enabling its application for industrial wastewater treatment.
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Published In
Journal of Hazardous, Toxic, and Radioactive Waste
Volume 20 • Issue 2 • April 2016
Copyright
© 2015 American Society of Civil Engineers.
History
Received: Jun 11, 2015
Accepted: Oct 14, 2015
Published online: Dec 17, 2015
Published in print: Apr 1, 2016
Discussion open until: May 17, 2016
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