Anaerobic Degradation of Cadaver Waste Using an Attached Growth Recycle System
Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 20, Issue 2
Abstract
In this study, mixed wastes composed of high levels of phenol, formalin, and ethanol emanating from biotechnology and health science laboratories on the Florida Gulf Coast University (FGCU) campus were degraded under anaerobic conditions in a hybrid suspended-attached growth system. Batch experiments were conducted using 80% removal of initial phenol concentrations as an end point. Formalin concentration was used to set the initial feeding constraints. After a stable, low level of phenol and chemical oxygen demand (COD) removal were established, three experimental periods demonstrated the simultaneous removal of 80% of influent COD, 97% removal of formalin, and at least 85% removal of phenol (concentrations of 1,000, 110, and , respectively). A biogas was produced continuously, but was not quantified nor identified because of constraints. Further research is needed to determine mineralization and possible intermediate formation and to prove such a system is a viable option for the on-site treatment of university or small-scale research-generated hazardous mixed wastes.
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Acknowledgments
The authors would like to acknowledge Algenol Biofuels for supplying the reactor supplies and R. Holtzclaw, Director of Environmental Health and Safety at FGCU, for supplying the hazardous waste solution and information. Additionally, the authors would like to thank L. Daniel, S. Goldman, L. Gosselin, M. Ledesma, and K. Richards for their help in the reactor design and COD, phenol, and formalin testing. This research was graciously funded by the Environmental Engineering Program of the Department of Environmental and Civil Engineering, Whitaker College of Engineering and Environmental Health and Safety, of FGCU.
References
Aronson, D., and Howard, P. H. (1997). Anaerobic biodegradation of organic chemicals in groundwater: A summary of field and laboratory studies, Environmental Science Center, Syracuse Research, North Syracuse, NY.
Bell, J., Young, E., and Stephen, S. (2012). “Phenol pathway (anaerobic).” 〈http://umbbd.ethz.ch/phe/phe_map.html〉 (Feb. 6, 2013).
Blum, D. J. W., and Speece, R. E. (1991). “A database of chemical toxicity to environmental bacteria and its use in interspecies comparisons and correlations.” Res. J. Water Pollut. Control Fed., 63(3), 198–207.
Chen, Y., Cheng, J. J., and Creamer, K. S. (2008). “Inhibition of anaerobic digestion process: A review.” Bioresour. Technol., 99(10), 4044–4064.
Chung, J., Bae, W., Lee, Y. W., and Rittmann, B. E. (2007). “Shortcut biological nitrogen removal in hybrid biofilm/suspended growth reactors.” Process Biochem., 42(3), 320–328.
Eaton, A. D., and Franson, M. A. H. (2005). “Standard methods for the examination of water and wastewater.” American Public Health Association, Washington, DC.
Fang, H. H. P., Chen, T., and Chan, O. C. (1995). “Toxic effects of phenolic pollutants on anaerobic benzoate-degrading granules.” Biotechnol. Lett., 17(1), 117–120.
Fedorak, P. M., and Hrudey, S. E. (1984). “The effects of phenol and some alkyl phenolics on batch anaerobic methanogenesis.” Water Res., 18(3), 361–367.
Filipe, C. D. M., and Grady, C. P. L., Jr. (1998). Biological wastewater treatment, CRC, Boca Raton, FL.
Jianlong, W., Hanchang, S., and Yi, Q. (2000). “Wastewater treatment in a hybrid biological reactor (HBR): Effect of organic loading rates.” Process Biochem., 36(4), 297–303.
Khan, S. J., Ilyas, S., Javid, S., Visvanathan, C., and Jegatheesan, V. (2011). “Performance of suspended and attached growth MBR systems in treating high strength synthetic wastewater.” Bioresour. Technol., 102(9), 5331–5336.
Lofty, H. R., and Rashed, I. G. (2002). “A method for treating wastewater containing formaldehyde.” Water Res., 36(3), 633–637.
Lu, Z., and Hegemann, W. (1998). “Anaerobic toxicity and biodegradation of formaldehyde in batch cultures.” Water Res., 32(1), 209–215.
MacKay, D. J. C. (2009). Sustainable energy-Without the hot air, UIT, Cambridge, U.K.
Oliveira, S. V. W. B., Moraes, E. M., Adorno, M. A. T., Varesche, M. B. A., Foresti, E., and Zaiat, M. (2004). “Formaldehyde degradation in an anaerobic packed-bed bioreactor.” Water Res., 38(7), 1685–1694.
Pereira, N. S., and Zaiat, M. (2009). “Degradation of formaldehyde in anaerobic sequencing batch biofilm reactor (ASBBR).” J. Hazard. Mater., 163(2–3), 777–782.
Pishgar, R., Najafpour, G., Navayi Neya, B., Mousavi, N., and Bakhsi, Z. (2011). “Anaerobic biodegradation of phenol: Comparative study on free and immobilized growth.” Iran. J. Energy Environ., 2(4), 348–355.
Saleem, Z. A. (1999). Anaerobic biodegradation rates of organic chemicals in groundwater: A summary of field and laboratory studies, U.S. EPA, Washington, DC.
Sayama, N., and Itokawa, Y. (1980). “Treatment of cresol, phenol and formalin using fixed-film reactors.” J. Appl. Bacteriol., 49(3), 395–403.
Seok, J.-H., and Komisar, S. J. (2003). “Regulatory role of n-propanol in propylene glycol biomethanization under overload.” Water Res., 37(7), 1515–1526.
Speece, R. E. (1996). Anaerobic biotechnology for industrial wastewaters, Archae, Nashville, TN.
U.S. EPA. (2002). “Toxicology review of phenol.” 〈http://www.epa.gov/iris/toxreviews/0088tr.pdf〉 (Feb. 6, 2013).
U.S. EPA. (2007a). “Formaldehyde—Hazard summary.” 〈http://www.epa.gov/ttnatw01/hlthef/formalde.html〉 (Feb. 6, 2013).
U.S. EPA. (2007b). “Phenol—Hazard summary.” 〈http://www.epa.gov/ttnatw01/hlthef/phenol.html〉 (Feb. 6, 2013).
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© 2015 American Society of Civil Engineers.
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Received: Feb 2, 2015
Accepted: Jul 21, 2015
Published online: Dec 8, 2015
Published in print: Apr 1, 2016
Discussion open until: May 8, 2016
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