Study on Nitrogen Transfer in the Symbiotic Ecosystem between Tubificidae and Microbes
Publication: Journal of Environmental Engineering
Volume 148, Issue 3
Abstract
A lab-scale study of the symbiotic ecosystem between Tubificidae and microbes (SETM) was carried out. The sludge reduction ability and ammonia removal performance of the system were investigated, and the nitrogen transfer pathway in the system was studied. In SETM, the substrates, microbes, and Tubificidae interacted with each other and reached equilibrium. During the experimental period (50 days), the amount of SETM sludge reduced by 30% compared to the control group (without Tubificidae). A considerable amount of nutrients was released in the presence of Tubificidae. Conceptual models of nitrogen flow were proposed, and the nitrogen transfer in the systems was described. In SETM, 21% more nitrogen was removed via assimilation compared with the control group, and the net nitrogen removal (including endogenous nitrogen) was higher than in the control group. The effective ammonia removal in SETM was 13% higher than in the control group. This result was supported by fluorescent in situ hybridization data showing that ammonia-oxidizing bacteria were 5% more prevalent in SETM than in the control group.
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Data Availability Statement
All data, models, and codes generated or used during the study appear in the published article.
Acknowledgments
This study was financed by Key Technologies R&D Programme of Zhejiang Province, China (2014C03002) and Natural Science Foundation of Zhejiang Province, China (Y12E08015).
References
APHA (American Public Health Association). 1998. Standard methods for the examination of water and wastewater. 20th ed. Washington, DC: APHA.
Berninger, U. G. 1990. The functioning and significance of microbial food webs in freshwater environments. Berlin: Freien Universität.
Brinkhurst, R. O. 1974. “Factors mediating interspecific aggregation of tubificid oligochaetes.” J. Fish. Res. Board Can. 31 (4): 460–462. https://doi.org/10.1139/f74-076.
Cai, L., D. Gao, K. Wang, H.-T. Liu, and X.-M. Wan. 2017. “Sludge reduction using aquatic worms under different aeration regimes.” Environ. Technol. Lett. 38 (6): 737–743. https://doi.org/10.1080/09593330.2016.1210241.
Coler, R. A., H. B. Gunner, and B. M. Zuckerman. 1967. “Selective feeding of tubificids on bacteria.” Nature 216 (5120): 1143–1144. https://doi.org/10.1038/2161143a0.
Coma, M., S. Puig, M. D. Balaguer, and J. Colprim. 2010. “The role of nitrate and nitrite in a granular sludge process treating low-strength wastewater.” Chem. Eng. J. 164 (1): 208–213. https://doi.org/10.1016/j.cej.2010.08.063.
Elissen, H. J. H. 2007. “Sludge reduction by aquatic worms in wastewater treatment: With emphasis on the potential application of Lumbriculus variegatus.” Ph.D. thesis, Dept. of Environmental Technology, Wageningen Univ.
Elissen, H. J. H., T. L. G. Hendrickx, H. Temmink, and C. J. N. Buisman. 2006. “A new reactor concept for sludge reduction using aquatic worms.” Water Res. 40 (20): 3713–3718. https://doi.org/10.1016/j.watres.2006.08.029.
Elissen, H. J. H., W. J. Mulder, T. L. G. Hendrickx, H. W. Elbersen, B. Beelen, H. Temmink, and C. J. N. Buisman. 2010. “Aquatic worms grown on biosolids: Biomass composition and potential applications.” Bioresour. Technol. 101 (2): 804–811. https://doi.org/10.1016/j.biortech.2009.08.060.
Emamjomeh, M. M., M. Tahergorabi, M. Farzadkia, and E. Bazrafshan. 2018. “A review of the use of earthworms and aquatic worms for reducing sludge produced: An innovative ecotechnology.” Waste Biomass Valorization 9 (9): 1877–2641. https://doi.org/10.1007/s12649-017-9907-z.
Guo, X.-S., J.-X. Liu, Y.-S. Wei, and L. Li. 2007. “Sludge reduction with Tubificidae and the impact on the performance of the wastewater treatment process.” J. Environ. Sci. 19 (3): 257–263. https://doi.org/10.1016/S1001-0742(07)60042-4.
Hendrickx, T. L. G., H. H. J. Elissen, H. Temmink, and C. J. N. Buisman. 2011. “Operation of an aquatic worm reactor suitable for sludge reduction at large scale.” Water Res. 45 (16): 4923–4929. https://doi.org/10.1016/j.watres.2011.06.031.
Hendrickx, T. L. G., H. Temmink, H. J. H. Elissen, and C. J. N. Buisman. 2009. “The effect of operating conditions on aquatic worms eating waste sludge.” Water Res. 43 (4): 943–950. https://doi.org/10.1016/j.watres.2008.11.034.
Hendrickx, T. L. G., H. Temmink, H. J. H. Elissen, and C. J. N. Buisman. 2010. “Aquatic worms eat sludge: Mass balances and processing of worm faeces.” J. Hazard. Mater. 177 (1–3): 633–638. https://doi.org/10.1016/j.jhazmat.2009.12.079.
Huang, X., P. Liang, and Y. Qian. 2007. “Excess sludge reduction induced by Tubifex tubifex in a recycled sludge reactor.” J. Biotechnol. 127 (3): 443–451. https://doi.org/10.1016/j.jbiotec.2006.07.035.
Juretschko, S., G. Timmermann, M. Schmid, K. H. Schleifer, A. Pommerening-Röser, H. P. Koops, and M. Wagner. 1998. “Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations.” Appl. Environ. Microbiol. 64 (8): 3042–3051. https://doi.org/10.1128/AEM.64.8.3042-3051.1998.
Khursheed, A., and A. A. Kazmi. 2011. “Retrospective of ecological approaches to excess sludge reduction.” Water Res. 45 (15): 4287–4310. https://doi.org/10.1016/j.watres.2011.05.018.
Li, X., M. Xing, J. Yang, and X. Dai. 2014. “Earthworm eco-physiological characteristics and quantification of earthworm feeding in vermifiltration system for sewage sludge stabilization using stable isotopic natural abundance.” J. Hazard. Mater. 276 (Jul): 353–361. https://doi.org/10.1016/j.jhazmat.2014.05.042.
Liu, X. W. 2007. “Technological optimization of sludge reduction based on T. tubifex predation.” [In Chinese.] Master’s thesis, School of Environmental and Municipal Engineering, Xi’an Univ. of Architecture and Technology.
Lou, J., Y. Cao, P. Sun, and P. Zheng. 2013. “The effects of operational conditions on the respiration rate of Tubificidae.” PLoS One 8 (12): e81219. https://doi.org/10.1371/journal.pone.0081219.
Lou, J., P. Sun, M. Guo, G. Wu, and Y. Song. 2011. “Simultaneous sludge reduction and nutrient removal (SSRNR) with interaction between Tubificidae and microorganisms: A full-scale study.” Bioresour. Technol. 102 (24): 11132–11136. https://doi.org/10.1016/j.biortech.2011.09.048.
Low, E. W., H. A. Chase, M. G. Milner, and T. P. Curtis. 2000. “Uncoupling of metabolism to reduce biomass production in the activated sludge process.” Water Res. 34 (12): 3204–3212. https://doi.org/10.1016/S0043-1354(99)00364-4.
Mahmood, T., and A. Elliott. 2006. “A review of secondary sludge reduction technologies for the pulp and paper industry.” Water Res. 40 (11): 2093–2112. https://doi.org/10.1016/j.watres.2006.04.001.
Ratsak, C. H., S. A. L. M. Koouman, and B. W. Kooi. 1993. “Modelling the growth of an oligochaete on activated sludge.” Water Res. 27 (5): 739–747. https://doi.org/10.1016/0043-1354(93)90136-6.
Ratsak, C. H., K. A. Maarsen, and S. A. L. M. Kooijman. 1996. “Effects of protozoa on carbon mineralization in activated sludge.” Water Res. 30 (1): 1–12. https://doi.org/10.1016/0043-1354(95)00096-4.
Rensink, J. H., and W. H. Rulkens. 1997. “Using metazoa to reduce sludge production.” Water Sci. Technol. 36 (11): 171–179. https://doi.org/10.2166/wst.1997.0408.
Strous, M., J. J. Heijnen, J. G. Kuenen, and M. S. M. Jetten. 1998. “The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms.” Appl. Microbiol. Biotechnol. 50 (5): 589–596. https://doi.org/10.1007/s002530051340.
Tchobanoglous, G., F. Burton, and H. D. Stensel. 2003. Metcalf and Eddy wastewater engineering: Treatment and reuse. New York: McGraw Hill.
Tian, Y., and Y. Lu. 2010. “Simultaneous nitrification and denitrification process in a new Tubificidae-reactor for minimizing nutrient release during sludge reduction.” Water Res. 44 (20): 6031–6040. https://doi.org/10.1016/j.watres.2010.07.069.
Tian, Y., Y. Lu, L. Chen, and H. Lin. 2010. “Optimization of process conditions with attention to the sludge reduction and stable immobilization in a novel Tubificidae-reactor.” Bioresour. Technol. 101 (15): 6069–6076. https://doi.org/10.1016/j.biortech.2010.03.011.
Wagner, M., R. Amann, P. Kampfer, B. Assmus, A. Hartmann, P. Hutzler, N. Springer, and K.-H. Schleifer. 1994. “Identification and in situ detection of gram-negative filamentous bacteria in activated sludge.” Syst. Appl. Microbiol. 17 (3): 405–417. https://doi.org/10.1016/S0723-2020(11)80058-5.
Wavre, M., and R. O. Brinkhurst. 1971. “Interactions between some tubificid oligochaetes and bacteria found in the sediments of Toronto Harbour, Ontario.” J. Fish. Res. Board Can. 28 (3): 335–341. https://doi.org/10.1139/f71-045.
Wei, Y., R. T. Van Houten, A. R. Borger, D. H. Eikelboom, and Y. Fan. 2003. “Minimization of excess sludge production for biological wastewater treatment.” Water Res. 37 (18): 4453–4467. https://doi.org/10.1016/S0043-1354(03)00441-X.
Wei, Y., Y. Wang, X. Guo, and J. Liu. 2009a. “Sludge reduction potential of the activated sludge process by integrating an oligochaete reactor.” J. Hazard. Mater. 163 (1): 87–91. https://doi.org/10.1016/j.jhazmat.2008.06.065.
Wei, Y., H. Zhu, Y. Wang, J. Li, P. Zhang, J. Hu, and J. Liu. 2009b. “Nutrients release and phosphorus distribution during oligochaetes predation on activated sludge.” Biochem. Eng. J. 43 (3): 239–245. https://doi.org/10.1016/j.bej.2008.10.004.
Xie, G.-J., B.-F. Liu, Q. Wang, J. Ding, and N.-Q. Ren. 2016. “Ultrasonic waste activated sludge disintegration for recovering multiple nutrients for biofuel production.” Water Res. 93 (Apr): 56–64. https://doi.org/10.1016/j.watres.2016.02.012.
Zhang, X., Y. Tian, Q. Wang, and H. Lin. 2013. “Waste sludge reduction using Limnodrilus hoffmeisteri: Growth, development and sludge predation potential of aquatic worm correlate with process conditions.” Ecol. Eng. 58 (Sep): 406–413. https://doi.org/10.1016/j.ecoleng.2013.07.003.
Zhang, X., H. Zeng, Q. Wang, J. Li, and C. Ma. 2020. “Sludge predation by aquatic worms: Physicochemical characteristics of sewage sludge and implications for dewaterability.” J. Cleaner Prod. 258 (9): 120612. https://doi.org/10.1016/j.jclepro.2020.120612.
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Published In
Journal of Environmental Engineering
Volume 148 • Issue 3 • March 2022
Copyright
© 2021 American Society of Civil Engineers.
History
Received: Jul 1, 2021
Accepted: Nov 7, 2021
Published online: Dec 21, 2021
Published in print: Mar 1, 2022
Discussion open until: May 21, 2022
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