Exogenous pH Buffer System with Addition Improving Thermophilic High-Solid Anaerobic Digestion of Waste-Activated Sludge
Publication: Journal of Environmental Engineering
Volume 147, Issue 2
Abstract
Volatile fatty acids (VFAs) and ammonia accumulation showed high inhibition for the high-solid anaerobic digestion (HSAD) of waste-activated sludge. This study investigated the effects of an exogenous pH buffer system with the addition of at different initial pH values in order to mitigate the inhibition of VFAs and ammonia accumulation. Results showed that the addition of with an optimal initial pH of 7.0 could prevent the pH from decreasing at the initial stage or rising at the final stage. In this study, 30.6% higher cumulative methane production was obtained compared to the control. A volatile solid (VS) removal rate as high as 42.3% was also achieved. Three key enzymes’ activities in the HSAD process were investigated, including alkaline protease, acetate kinase, and Coenzyme . Moreover, a model simulation presented that a modified Gompertz model fit the actual methane production best, as supported by the highest value. The addition of phosphorus buffer substantially increased the hydrolysis rate () and maximum methane potential () by parameters analysis, which explained the increased VS removal and methane production. High-throughput sequencing analysis clearly showed differences in the microbial community between samples from different treatments. Tepidimicrobium and Rhodobacter were the dominant bacterial genera involved in the HSAD system, which presented a high degradation capacity of organic material. The archaea community was dominated by Methanosaeta and Methanoculleus, and these are the typical aceticlastic methanogens and hydrogenotrophic methanogens, respectively. Herein, addition proved to be an effective method to overcome the problems related to the free ammonia and VFA accumulation during the sludge HSAD process under thermophilic conditions on a lab-scale setting (using a 7-L bioreactor).
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Data Availability Statement
All data, models, and code generated or used during the study appear in the published article.
Acknowledgments
This work was financially supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China (No. 2017ZX07403002-03) and the National Natural Science Foundation of China (No. 21876110).
References
Abdulkarim, B. I., and M. E. Abdullahi. 2010. “Effect of buffer () and waste type in high solid thermophilic anaerobic digestion.” Int. J. ChemTech Res. 2 (2): 980–984.
APHA (American Public Health Association). 1998. Standard methods for the examination of water and wastewater. 20th ed. Washington, DC: APHA.
Aymerich, E., M. Esteban-Gutiérrez, and L. Sancho. 2013. “Analysis of the stability of high-solids anaerobic digestion of agro-industrial waste and sewage sludge.” Bioresour. Technol. 144 (Sep): 107–114. https://doi.org/10.1016/j.biortech.2013.06.074.
Baccay, R. A., and A. G. Hashimoto. 1984. “Acidogenic and methanogenic fermentation of causticized straw.” Biotechnol. Bioeng. 26 (8): 885–891. https://doi.org/10.1002/bit.260260811.
Cheng, Y., S. Sang, H. Huang, X. Liu, and J. Ouyang. 2007. “Variation of coenzyme F420 activity and methane yield in landfill simulation of organic waste.” J. China Univ. Mining Technol. 17 (3): 0403–0408. https://doi.org/10.1016/S1006-1266(07)60114-X.
Dai, X. H., N. N. Duan, B. Dong, and L. L. Dai. 2013. “High-solids anaerobic co-digestion of sewage sludge and food waste in comparison with mono digestions: Stability and performance.” Waste Manage. 33 (2): 308–316. https://doi.org/10.1016/j.wasman.2012.10.018.
Duan, N. N., B. Dong, B. Wu, and X. H. Dai. 2012. “High-solid anaerobic digestion of sewage sludge under mesophilic conditions: Feasibility study.” Bioresour. Technol. 104 (Jan): 150–156. https://doi.org/10.1016/j.biortech.2011.10.090.
El-Mashad, H. M. 2013. “Kinetics of methane production from the co-digestion of switch grass and Spirulina platensis algae.” Bioresour. Technol. 132 (Mar): 305–312. https://doi.org/10.1016/j.biortech.2012.12.183.
Garcia, J. L., B. K. C. Patel, and B. Ollivier. 2000. “Taxonomic, phylogenetic, and ecological diversity of methanogenic archaea.” Anaerobe 6 (4): 205–226. https://doi.org/10.1006/anae.2000.0345.
Goel, R., T. Mino, H. Satoh, and T. Matsuo. 1998. “Enzyme activities under anaerobic and aerobic conditions in activated sludge sequencing batch reactor.” Water Res. 32 (7): 2081–2088. https://doi.org/10.1016/S0043-1354(97)00425-9.
Guendouz, J., P. Buffiere, J. Cacho, M. Carrere, and J. P. Delgenes. 2008. “High-solids anaerobic digestion: Comparison of three pilot scales.” Water Sci. Technol. 58 (9): 1757–1763. https://doi.org/10.2166/wst.2008.521.
Guo, J., Y. Peng, B. J. Ni, X. Han, L. Fan, and Z. Yuan. 2015. “Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing.” Microb. Cell Fact. 14: 33. https://doi.org/10.1186/s12934-015-0218-4.
Hansen, K. H., I. Angelidaki, and B. K. Ahring. 1998. “Anaerobic digestion of swine manure: Inhibition by ammonia.” Water Res. 32 (1): 5–12. https://doi.org/10.1016/S0043-1354(97)00201-7.
Horiuchi, J., T. Shimizu, T. Kanno, and M. Kobayashi. 1999. “Dynamic behavior in response to pH shift during anaerobic acidogenesis with a chemostat culture.” Biotechnol. Tech. 13 (3): 155–157. https://doi.org/10.1023/A:1008947712198.
Hosseini, K. E., T. Johnson, and C. Eskicioglu. 2017. “Advanced anaerobic digestion of municipal sludge using a novel and energy-efficient radio frequency pretreatment system.” Water Res. 118 (Jul): 70–81. https://doi.org/10.1016/j.watres.2017.04.017.
Inanc, B., S. Matsui, and S. Ide. 1996. “Propionic acid accumulation and controlling factors in anaerobic treatment of carbohydrate: Effect of and pH.” Water Sci. Tech. 34 (5–6): 317–325. https://doi.org/10.2166/wst.1996.0566.
Jolis, D. 2008. “High-solids anaerobic digestion of municipal sludge pretreated by thermal hydrolysis.” Water Environ. Res. 80 (7): 654–662. https://doi.org/10.2175/193864708X267414.
Li, C., X. D. Wang, G. Y. Zhang, G. W. Yu, J. J. Lin, and Y. Wang. 2017. “Hydrothermal and alkaline hydrothermal pretreatments plus anaerobic digestion of sewage sludge for dewatering and biogas production: Bench-scale research and pilot-scale verification.” Water Res. 117 (Jun): 49–57. https://doi.org/10.1016/j.watres.2017.03.047.
Li, K., R. H. Liu, and S. Chen. 2015. “Comparison of anaerobic digestion characteristics and kinetics of four livestock manures with different substrate concentrations.” Bioresour. Technol. 198 (Dec): 133–140. https://doi.org/10.1016/j.biortech.2015.08.151.
Liang, Z., W. Li, S. Yang, and P. Du. 2010. “Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge.” Chemosphere 81 (5): 626–632. https://doi.org/10.1016/j.chemosphere.2010.03.043.
Liao, X. C., and H. Li. 2015. “Biogas production from low-organic-content sludge using a high-solids anaerobic digester with improved agitation.” Appl. Energy 148 (Jun): 252–259. https://doi.org/10.1016/j.apenergy.2015.03.082.
Liao, X. C., H. Li, Y. Y. Zhang, C. Liu, and Q. W. Chen. 2016. “Accelerated high-solids anaerobic digestion of sewage sludge using low-temperature thermal pretreatment.” Int. Biodeterior. Biodegrad. 106 (Jan): 141–149. https://doi.org/10.1016/j.ibiod.2015.10.023.
Liu, C., H. Li, Y. Y. Zhang, and Q. W. Chen. 2016. “Characterization of methanogenic activity during high-solids anaerobic digestion of sewage sludge.” Biochem. Eng. J. 109 (May): 96–100. https://doi.org/10.1016/j.bej.2016.01.010.
Lu, X., G. Zhen, Y. Liu, T. Hojo, A. L. Estrada, and Y. Y. Li. 2014. “Long-term effect of the antibiotic cefalexin on methane production during waste activated sludge anaerobic digestion.” Bioresour. Technol. 169 (Oct): 644–651. https://doi.org/10.1016/j.biortech.2014.07.056.
Mcmahon, K. D., P. G. Stroot, R. I. Mackie, and L. Raskin. 2001. “Anaerobic co-digestion of municipal solid waste and biosolids under various mixing conditions—II: Microbial population dynamics.” Water Res. 35 (7): 1817–1827. https://doi.org/10.1016/S0043-1354(00)00438-3.
Meng, L., W. Li, S. Zhang, C. Wu, W. Jiang, and C. Sha. 2016. “Effect of different extra carbon sources on nitrogen loss control and the change of bacterial populations in sewage sludge composting.” Ecol. Eng. 94 (Sep): 238–243. https://doi.org/10.1016/j.ecoleng.2016.05.013.
Meng, X. S., D. W. Yu, Y. S. Wei, Y. X. Zhang, Q. F. Zhang, Z. Y. Wang, J. B. Liu, and Y. W. Wang. 2018. “Endogenous ternary pH buffer system with ammonia-carbonates-VFAs in high solid anaerobic digestion of swine manure: An alternative for alleviating ammonia inhibition?” Process Biochem. 69 (Jun): 144–152. https://doi.org/10.1016/j.procbio.2018.03.015.
Meng, X. Y., X. F. Yuan, J. W. Ren, X. F. Wang, W. B. Zhu, and Z. J. Cui. 2017. “Methane production and characteristics of the microbial community in a two-stage fixed-bed anaerobic reactor using molasses.” Bioresour. Technol. 241 (Oct): 1050–1059. https://doi.org/10.1016/j.biortech.2017.05.181.
Nelson, M. C., H. G. Benjamino, S. L. Grim, and J. Graf. 2014. “Analysis, optimization and verification of illumina-generated 16s rRNA gene amplicon surveys.” PLoS One 9 (4): e94249. https://doi.org/10.1371/journal.pone.0094249.
Neumann, P., A. Torres, F. G. Fermoso, R. Borja, and D. Jeison. 2015. “Anaerobic codigestion of lipid-spent microalgae with waste activated sludge and glycerol in batch mode.” Int. Biodeterior. Biodegrad. 100 (May): 85–88. https://doi.org/10.1016/j.ibiod.2015.01.020.
Nghiem, L. D., T. T. Nguyen, P. Manassa, S. K. Fitzgerald, M. Dawson, and S. Vierboom. 2014. “Co-digestion of sewage sludge and crude glycerol for on-demand biogas production.” Int. Biodeterior. Biodegrad. 95 (Nov): 160–166. https://doi.org/10.1016/j.ibiod.2014.04.023.
Niu, L. L., L. Song, X. L. Liu, and X. Z. Dong. 2009. “Tepidimicrobium xylanilyticum sp. nov., an anaerobic xylanolytic bacterium, and emended description of the genus Tepidimicrobium.” Int. J Syst. Evol. Microbiol. 59 (11): 2698–2701. https://doi.org/10.1099/ijs.0.005124-0.
Niu, Q. G., W. Qiao, H. Qiang, and Y. Y. Li. 2013. “Microbial community shifts and biogas conversion computation during steady, inhibited and recovered stages of thermophilic methane fermentation on chicken manure with a wide variation of ammonia.” Bioresour. Technol. 146 (Oct): 223–233. https://doi.org/10.1016/j.biortech.2013.07.038.
Pender, S., M. Toomey, M. Carton, D. Eardly, and J. W. Patching. 2004. “Long-term effects of operating temperature and sulphate addition on the metanogenic community structure of anaerobic hybrid reactors.” Water Res. 38 (3): 619–630. https://doi.org/10.1016/j.watres.2003.10.055.
Ratanatamskul, C., G. Onnum, and K. Yamamoto. 2014. “A prototype single-stage anaerobic digester for co-digestion of food waste and sewage sludge from high-rise building for on-site biogas production.” Int. Biodeterior. Biodegrad. 95 (Nov): 176–180. https://doi.org/10.1016/j.ibiod.2014.06.010.
Santosh, Y., T. R. Sreekrishnan, S. Kohli, and V. Rana. 2004. “Enhancement of biogas production from solid substrates using different techniques—A review.” Bioresour. Technol. 95 (1): 1–10. https://doi.org/10.1016/j.biortech.2004.02.010.
Shou, Z. Q., N. W. Zhu, H. P. Yuan, X. H. Dai, and Y. W. Shen. 2019. “Buffering phosphate mitigates ammonia emission in sewage sludge composting: Enhanced organics removal coupled with microbial ammonium assimilation.” J. Cleaner Prod. 227 (Aug): 189–198. https://doi.org/10.1016/j.jclepro.2019.04.197.
Sun, C., W. Cao, C. J. Banks, S. Heaven, and R. H. Liu. 2016a. “Biogas production from undiluted chicken manure and maize silage: A study of ammonia inhibition in high solids anaerobic digestion.” Bioresour. Technol. 218 (Oct): 1215–1223. https://doi.org/10.1016/j.biortech.2016.07.082.
Sun, H., S. B. Wu, and R. J. Dong. 2016b. “Monitoring volatile fatty acids and carbonate alkalinity in anaerobic digestion: Titration methodologies.” Chem. Eng. Technol. 39 (4): 599–610.
USEPA. 1993. Standards for the use or disposal of sewage sludge. USEPA 40CFR PART 503. Washington, DC: USEPA.
Wang, S., and Y. Zeng. 2018. “Ammonia emission mitigation in food waste composting: A review.” Bioresour. Technol. 248 (Jan): 13–19. https://doi.org/10.1016/j.biortech.2017.07.050.
Westerholm, M., T. Liu, and A. Schnurer. 2020. “Comparative study of industrial-scale high-solid biogas production from food waste: Process operation and microbiology.” Bioresour. Technol. 304 (May): 122981. https://doi.org/10.1016/j.biortech.2020.122981.
Wu, P., Y. Zhang, Z. B. Chen, Y. L. Wang, F. F. Zhu, B. Cao, Y. Wu, and N. Li. 2019. “The organophosphorus pesticides in soil was degradated by Rhodobacter sphaeroides after wastewater treatment.” Biochem. Eng. J. 141 (Jan): 247–251. https://doi.org/10.1016/j.bej.2018.07.019.
Yin, X. B., L. W. Lian, J. Q. Xu, and Y. H. Ke. 1998. “Unique enzymes and biochemical monitoring methods in methanogenesis.” China Biogas 10 (3): 8–13.
Yu, B., Z. Y. Lou, D. L. Zhang, A. D. Shan, H. P. Yuan, N. W. Zhu, and K. H. Zhang. 2015. “Variations of organic matters and microbial community in thermophilic anaerobic digestion of waste activated sludge with the addition of ferric salts.” Bioresour. Technol. 179 (Mar): 291–298. https://doi.org/10.1016/j.biortech.2014.12.011.
Yuan, H. P., C. W. Xu, and N. W. Zhu. 2014. “Disinhibition of the ammonium nitrogen in autothermal thermophilic aerobic digestion for sewage sludge by chemical precipitation.” Bioresour. Technol. 169 (Oct): 686–691. https://doi.org/10.1016/j.biortech.2014.07.016.
Yuan, H. P., and N. W. Zhu. 2016. “Progress in inhibition mechanisms and process control of intermediates and by-products in sewage sludge anaerobic digestion.” Renewable Sustainable Energy Rev. 58 (May): 429–438. https://doi.org/10.1016/j.rser.2015.12.261.
Zhang, D. L., H. P. Yuan, B. Yu, X. H. Dai, X. T. Huang, Z. Y. Lou, and N. W. Zhu. 2016. “Performance and microbial communities of a batch anaerobic reactor treating liquid 1and high-solid sludge at thermophilic conditions.” RSC Adv. 6 (101): 99524–99531. https://doi.org/10.1039/C6RA21111A.
Zhang, S. T., H. G. Guo, L. Z. Du, J. F. Liang, X. B. Lu, N. Li, and K. Q. Zhang. 2015. “Influence of NaOH and thermal pretreatment on dewatered activated sludge solubilisation and subsequent anaerobic digestion: Focused on high-solid state.” Bioresour. Technol. 185 (Jun): 171–177. https://doi.org/10.1016/j.biortech.2015.02.050.
Zhang, W., Q. Wei, S. Wu, D. Qi, W. Li, Z. Zuo, and R. Dong. 2014. “Batch anaerobic codigestion of pig manure with dewatered sewage sludge under mesophilic conditions.” Appl. Energy 128 (Sep): 175–183. https://doi.org/10.1016/j.apenergy.2014.04.071.
Zhen, G. Y., X. Q. Lu, Y. Y. Li, and Y. C. Zhao. 2014. “Combined electrical-alkali pretreatment to increase the anaerobic hydrolysis rate of waste activated sludge during anaerobic digestion.” Appl. Energy 128 (Sep): 93–102. https://doi.org/10.1016/j.apenergy.2014.04.062.
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Journal of Environmental Engineering
Volume 147 • Issue 2 • February 2021
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© 2020 American Society of Civil Engineers.
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Received: May 19, 2020
Accepted: Oct 13, 2020
Published online: Dec 11, 2020
Published in print: Feb 1, 2021
Discussion open until: May 11, 2021
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