Improving the Performance of Attached-Growth Wastewater Treatment Processes by Altering the Support Media Surface
Publication: Journal of Environmental Engineering
Volume 145, Issue 8
Abstract
This study investigated the impact of altering the surface characteristics of support media used in attached-growth processes on the performance of biological wastewater treatment. The surface alteration was achieved through physical and chemical treatment of high-density polyethylene (HDPE), and changes in the HDPE surface characteristics, biofilm attachment, biofilm growth, and wastewater treatment performance were evaluated. Physical treatment procedures included roughening the HDPE coupons with sandpaper, and chemical treatment processes involved exposing the HDPE coupons to hydrogen peroxide and nitric acid. Changes in the physicochemical characteristics of the HDPE surfaces were determined using a variable pressure scanning electron microscope (VPSEM), a confocal profile meter, and contact angle (CA) measurements. The biofilm attachment, growth, and thickness were monitored using VPSEM images, biofilm volatile and total solids, protein quantities, and live/dead assay. The performance of biofilms in treating synthetic wastewater was determined by measuring the changes in chemical oxygen demand, ammonia, and total phosphorus concentrations. The results showed that physical treatment of the HDPE coupons increased the surface roughness and CA, changed the surface characteristics from hydrophilic to hydrophobic, improved the biofilm attachment and growth, increased the percentage of live cells, and enhanced the wastewater treatment performance. Chemical treatment of the HDPE coupons caused some minor changes to the surface and did not lead to significant improvements in biofilm growth or wastewater treatment.
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Acknowledgments
This study was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) under the ENGAGE program (Grant No. EGP 453350-13).
References
Almomani, F. A., R. Delatolla, and B. Örmeci. 2014. “Field study of moving bed biofilm reactor technology for post-treatment of wastewater lagoon effluent at 1°C.” Environ. Technol. 35 (13): 1596–1604. https://doi.org/10.1080/09593330.2013.874500.
Almomani, F. A., and M. A. M. Khraisheh. 2016. “Treatment of septic tank effluent using moving-bed biological reactor: Kinetic and biofilm morphology.” Int. J. Environ. Sci. Technol. 13 (8): 1917–1932. https://doi.org/10.1007/s13762-016-1039-7.
Al Momani, F., O. Gonzalez, C. Sans, and S. Esplugas. 2004. “Combining photo-Fenton process with biological sequencing batch reactor for 2, 4-dichlorophenol degradation.” Water Sci. Technol. 49 (4): 293–298. https://doi.org/10.2166/wst.2004.0288.
Alsteens, D., E. Dague, P. G. Rouxhet, A. R. Baulard, and Y. F. Dufrane. 2007. “Direct measurement of hydrophobic forces on cell surfaces using AFM.” Langmuir 23 (24): 11977–11979. https://doi.org/10.1021/la702765c.
An, Y. H., and R. J. Friedman. 1998. “Concise review of mechanisms of bacterial adhesion to biomaterial surfaces.” J. Biomed. Mater. Res. 43 (3): 338–348. https://doi.org/10.1002/(SICI)1097-4636(199823)43:3%3C338::AID-JBM16%3E3.0.CO;2-B.
Antonie, R. 1976. Fixed biological surfaces—Wastewater treatment. Boca Raton, FL: CRC Press.
APHA, AWWA, and WEF (American Public Health Association, American Water Works Association, and Water Environment Federation). 2017. Standard methods for the examination of water and wastewater. 23rd ed. Washington, DC: APHA.
Apilánez, I., A. Gutiérrez, and M. Díaz. 1998. “Effects of surface materials on initial biofilm development.” Bioresour. Technol. 66 (3): 225–230. https://doi.org/10.1016/S0960-8524(98)00052-2.
Azeredo, J., and R. Oliveira. 2000. “The role of exopolymers in the attachment of Sphingomonas paucimobilis.” Biofouling 16 (1): 59–67. https://doi.org/10.1080/08927010009378430.
Azizi, S., A. Valipour, and T. Sithebe. 2013. “Evaluation of different wastewater treatment processes and development of a modified attached growth bioreactor as a decentralized approach for small communities.” Sci. World J. 2013: 1–8. https://doi.org/10.1155/2013/156870.
Bachmann, R. T., and R. G. J. Edyvean. 2006. “AFM study of the colonisation of stainless steel by Aquabacterium commune.” Int. Biodeterior. Biodegrad. 58 (3–4): 112–118. https://doi.org/10.1016/j.ibiod.2006.06.008.
Barnharst, T., A. Rajendran, and B. Hum. 2018. “Bioremediation of synthetic intensive aquaculture wastewater by a novel feed-grade composite biofilm.” Int. Biodeterior. Biodegrad. 126: 131–142. https://doi.org/10.1016/j.ibiod.2017.10.007.
Bhushan, B., and Y. C. Jung. 2007. “Wetting study of patterned surfaces for superhydrophobicity.” Ultramicroscopy 107 (10–11): 1033–1041. https://doi.org/10.1016/j.ultramic.2007.05.002.
Bories, A., J. Raynal, and F. Bazile. 1988. “Anaerobic digestion of high-strength distillery wastewater (cane molasses stillage) in a fixed-film reactor.” Biol. Wastes 23 (4): 251–267. https://doi.org/10.1016/0269-7483(88)90014-6.
Bos, R., H. C. Van der Mei, and H. J. Busscher. 1999. “Physico-chemistry of initial microbial adhesive interactions—Its mechanisms and methods for study.” FEMS Microbiol. Rev. 23 (2): 179–230. https://doi.org/10.1016/S0168-6445(99)00004-2.
Brizzolara, R. A., and E. R. Holm. 2006. “The effect of solid surface tension and exposure to elevated hydrodynamic shear on Pseudomonas fluorescens biofilms grown on modified titanium surfaces.” Biofouling 22 (6): 431–440. https://doi.org/10.1080/08927010601053616.
Bruinsma, G. M., H. C. Van der Mei, and H. J. Busscher. 2001. “Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses.” Biomaterials 22 (24): 3217–3224. https://doi.org/10.1016/S0142-9612(01)00159-4.
Callow, M. E., and R. L. Fletcher. 1994. “The influence of low surface energy materials on bioadhesion—A review.” Int. Biodeterior. Biodegrad. 34 (3–4): 333–348. https://doi.org/10.1016/0964-8305(94)90092-2.
Cerca, N., G. B. Pier, M. Vilanova, R. Oliveira, and J. Azeredo. 2005. “Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis.” Res. Microbiol. 156 (4): 506–514. https://doi.org/10.1016/j.resmic.2005.01.007.
Chai, Q., and B. Lie. 2008. “Predictive control of an intermittently aerated activated sludge process in 2008.” In Proc., American Control Conf., 2209–2214. New York: IEEE.
Characklis, W. G., and K. C. Marshall. 1990. Biofilms: A basis for an interdisciplinary approach. In Biofilms, edited by W. G. Characklis and K. C. Marshall, 3–16. New York: John Wiley & Sons.
Davidson, C., and C. Lowe. 2004. “Optimisation of polymeric surface pre-treatment to prevent bacterial biofilm formation for use in microfluidics.” J. Mol. Recognit. 17 (3): 180–185. https://doi.org/10.1002/jmr.662.
Fletcher, M. M. 1980. Microbial adhesion to surfaces. Chichester, UK: Ellis Horwood.
Fonseca, A. P., P. L. Granja, J. A. Nogueira, D. R. Oliveira, and M. A. Barbosa. 2001. “Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives.” J. Mater. Sci.: Mater. Med. 12 (6): 543–548. https://doi.org/10.1023/A:1011227915575.
Garrett, T. R., M. Bhakoo, and Z. Zhang. 2008. “Bacterial adhesion and biofilms on surfaces.” Prog. Nat. Sci. 18 (9): 1049–1056. https://doi.org/10.1016/j.pnsc.2008.04.001.
Goyal, S., R. Seth, and B. Handa. 1996. “Diphasic fixed-film biomethanation of distillery spentwash.” Bioresour. Technol. 56 (2–3): 239–244. https://doi.org/10.1016/0960-8524(96)00033-8.
Grady, C. P. L., Jr., G. T. Daigger, N. G. Love, and C. D. M. Filipe. 2011. Biological wastewater treatment. Boca Raton, FL: CRC Press.
Gubner, R., and I. B. Beech. 2000. “The effect of extracellular polymeric substances on the attachment of Pseudomonas NCIMB 2021 to AISI 304 and 316 stainless steel.” Biofouling 15 (1–3): 25–36. https://doi.org/10.1080/08927010009386295.
Habouzit, F., G. Gévaudan, J. Hamelin, J. Steyer, and N. Bernet. 2011. “Influence of support properties on the potential selection of Archaea during initial adhesion of a methanogenic consortium.” Bioresour. Technol. 102 (5): 4054–4060. https://doi.org/10.1016/j.biortech.2010.12.023.
Henze, M., P. Harremoes, J. La Cour Jansen, and E. Arvin. 2001. Wastewater treatment: Biological and chemical processes. Berlin: Springer.
Hermansson, M. 1999. “The DLVO theory in microbial adhesion.” Colloids Surf., B 14 (1–4): 105–119. https://doi.org/10.1016/S0927-7765(99)00029-6.
Hori, K., and S. Matsumoto. 2010. “Bacterial adhesion: From mechanism to control.” Biochem. Eng. J. 48 (3): 424–434. https://doi.org/10.1016/j.bej.2009.11.014.
Jucker, B., A. Zehnder, and H. Harms. 1998. “Quantification of polymer interactions in bacterial adhesion.” Environ. Sci. Technol. 32 (19): 2909–2915. https://doi.org/10.1021/es980211s.
Katsikogianni, M., and Y. Missirlis. 2004. “Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria material interactions.” Eur. Cells Mater. 8: 37–57. https://doi.org/10.22203/eCM.v008a05.
Kermani, M., B. Bina, H. Movahedian, M. Amin, and M. Nikaeinm. 2008. “Application of moving bed biofilm process for biological organics and nutrients removal from municipal wastewater.” Am. J. Environ. Sci. 4 (6): 675–682. https://doi.org/10.3844/ajessp.2008.675.682.
Khan, M. M., T. Chapman, and K. Cochran. 2013. “Attachment surface energy effects on nitrification and estrogen removal rates by biofilms for improved wastewater treatment.” Water Res. 47 (7): 2190–2198. https://doi.org/10.1016/j.watres.2013.01.036.
Khan, S. J., S. Ilyas, S. Javid, C. Visvanathan, and V. Jegatheesan. 2011. “Performance of suspended and attached growth MBR systems in treating high strength synthetic wastewater.” Bioresour. Technol. 102 (9): 5331–5336. https://doi.org/10.1016/j.biortech.2010.09.100.
Koch, K., and W. Barthlott. 2009. “Superhydrophobic and superhydrophilic plant surfaces: An inspiration for biomimetic materials.” Philos. Trans. R. Soc., A 367 (1893): 1487–1509. https://doi.org/10.1098/rsta.2009.0022.
Koerner, R. J., L. A. Butterworth, I. V. Mayer, R. Dasbach, and H. J. Busscher. 2002. “Bacterial adhesion to titanium-oxy-nitride (TiNOx) coatings with different resistivities: A novel approach for the development of biomaterials.” Biomaterials 23 (14): 2835–2840. https://doi.org/10.1016/S0142-9612(01)00404-5.
Li, B., and B. E. Logan. 2004. “Bacterial adhesion to glass and metal-oxide surfaces.” Colloids Surf., B 36 (2): 81–90. https://doi.org/10.1016/j.colsurfb.2004.05.006.
Lorite, G. S., C. M. Rodrigues, A. A. De Souza, C. Kranz, B. Mizaikoff, and M. A. Cotta. 2011. “The role of conditioning film formation and surface chemical changes on Xylella fastidiosa adhesion and biofilm evolution.” J. Colloid Interface Sci. 359 (1): 289–295. https://doi.org/10.1016/j.jcis.2011.03.066.
Loupasaki, E., and E. Diamadopoulos. 2013. “Attached growth systems for wastewater treatment in small and rural communities: A review.” J. Chem. Technol. Biotechnol. 88 (2): 190–204. https://doi.org/10.1002/jctb.3967.
Nie, Q., and F. Gu. 2010. “Preparation of composite carrier material immobilized activated sludge and application in treating wastewater.” Adv. Mater. Res. 113–116, 301–304.
Oh, Y. J., N. R. Lee, N. R. Jo, W. K. Jung, and J. S. Lim. 2009. “Effects of substrates on biofilm formation observed by atomic force microscopy.” Ultramicroscopy 109 (8): 874–880. https://doi.org/10.1016/j.ultramic.2009.03.042.
Oliveira, R., J. Azeredo, P. Texeira, and A. P. Fonseca. 2001. The role of hydrophobicity in bacterial adhesion. In Biofilm community interactions: Chance or necessity? edited by P. Gilbert, D. Allison, M. Brading, J. Verran, and J. Walker, 11–22. Cardiff, UK: Biofilm Community BioLine.
Pell, M., and F. Nyberg. 1989. “Infiltration of wastewater in a newly started pilot sand-filter system: I. Reduction of organic matter and phosphorus.” J. Environ. Qual. 18 (4): 451–457. https://doi.org/10.2134/jeq1989.00472425001800040009x.
Ramothokang, T. R., G. D. Drysdale, and F. Bux. 2003. “Isolation and cultivation of filamentous bacteria implicated in activated sludge bulking.” Water SA 29 (4): 405–410.
Rittmann, B. E., and P. L. McCarty. 2001. Environmental biotechnology. New York: McGraw-Hill.
Sanabria, J. 2014. “Environmental biotechnology research: Challenges and opportunities in Latin America.” J. Agric. Environ. Ethics 27 (4): 681–694. https://doi.org/10.1007/s10806-014-9502-2.
Sheng, X., Y. P. Ting, and S. O. Pehkonen. 2008. “The influence of ionic strength, nutrients and pH on bacterial adhesion to metals.” J. Colloid Interface Sci. 321 (2): 256–264. https://doi.org/10.1016/j.jcis.2008.02.038.
Sousa, M., J. Azeredo, J. Feijó, and R. Oliveira. 1997. “Polymeric supports for the adhesion of a consortium of autotrophic nitrifying bacteria.” Biotechnol. Tech. 11 (10): 751–754. https://doi.org/10.1023/A:1018400619440.
Spellman, F. R. 2008. Handbook of water and wastewater treatment plant operations. New York: CRC Press.
Stephenson, T., A. Mann, and J. Upton. 1993. “The small footprint wastewater treatment process.” Chem. Ind. 14: 533–536.
Teixeira, P., and R. Oliveira. 1999. “Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates.” J. Adhes. Sci. Technol. 13 (11): 1287–1294. https://doi.org/10.1163/156856199X00190.
Van Oss, C. J. 1995. “Hydrophobicity of biosurfaces—Origin, quantitative determination and interaction energies.” Colloids Surf., B 5 (3–4): 91–110. https://doi.org/10.1016/0927-7765(95)01217-7.
Verma, M., S. K. Brar, J. F. Blais, R. D. Tyagi, and R. Y. Surampalli. 2006. “Aerobic biofiltration processes—Advances in wastewater treatment.” Pract. Period. Hazard. Toxic Radioact. Waste Manage. 10 (4): 264–276. https://doi.org/10.1061/(ASCE)1090-025X(2006)10:4(264).
Yang, Y., C. Tada, M. S. Miah, K. Tsukahara, T. Yagishita, and S. Sawayama. 2004. “Influence of bed materials on methanogenic characteristics and immobilized microbes in anaerobic digester.” Mater. Sci. Eng., C 24 (3): 413–419. https://doi.org/10.1016/j.msec.2003.11.005.
Young, J. C., and B. S. Yang. 1989. “Design considerations for full-scale anaerobic filters.” Res. J. Water Pollut. Control Fed. 61 (9/10): 1576–1587.
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©2019 American Society of Civil Engineers.
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Received: Jun 13, 2018
Accepted: Dec 7, 2018
Published online: Jun 6, 2019
Published in print: Aug 1, 2019
Discussion open until: Nov 6, 2019
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