Phosphorus Supplementation Alleviation of the Cadmium-Induced Toxicity by Modulating Oxidative Stress Mechanisms in Vetiver Grass [Chrysopogon zizanioides (L.) Roberty]
Publication: Journal of Environmental Engineering
Volume 142, Issue 9
Abstract
The present study describes the potential role of additional phosphorus (P) on alleviating cadmium (Cd)–induced toxicity in vetiver plants. The plant growth was significantly affected at Cd treatment compared to the control, while it increased by 13% with addition of P in the medium when compared with Cd treatment. Accumulation of Cd content in plants showed positive correlation with Cd and combination exposure, whereas it was slightly decreased at higher doses of combination treatments in roots compared to the shoot exposed to Cd. A decreased level of photosynthetic pigments was noticed with increasing the Cd concentrations, but it was slightly enhanced with Cd and P combination treatments over Cd treatment. Both hydrogen peroxide () and malondialhyde (MDA) contents were found to be increased with increasing Cd dose. The antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) activities were found to be increased at lower doses of Cd exposure, while they were slightly decreased at higher doses of Cd treatment. The ameliorating effect of P was confirmed by reduction of , MDA contents, and antioxidative enzyme activities in shoot and root tissues. Isozyme banding pattern revealed the appearance of additional isoforms of SOD, CAT, and POX in leaf tissues grown in combination. These results together suggested that the P amendment with Cd may decrease the Cd-induced toxicity that could promote seedlings growth. The present protocol could be considered as novel technology for alleviation of heavy metal stress-induced toxicity in plants including Cd ions.
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Acknowledgments
The University Grants Commission, New Delhi, India [F. No. 41-13/2012(SR)] is gratefully acknowledged for providing financial assistance.
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Published In
Journal of Environmental Engineering
Volume 142 • Issue 9 • September 2016
Copyright
© 2016 American Society of Civil Engineers.
History
Received: Mar 24, 2015
Accepted: Dec 30, 2015
Published online: Apr 4, 2016
Published in print: Sep 1, 2016
Discussion open until: Sep 4, 2016
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