Biofilm Reactor for Removal: Review with Challenges and A Study with Freeze Dried Bacteria
Publication: Journal of Environmental Engineering
Volume 142, Issue 9
Abstract
The present article intends to make a state-of-the review of removal of ions from wastewater using biofilm reactor. Preservation of mercury resistant bacteria has been identified as one of the challenges regarding commercialization of biofilm reactors for this purpose. Finally, a case study on biofilm reactor using freeze-dried Bacillus cereus (JUBT1) has been incorporated. A novel, cost-effective, and economically feasible freeze-drying protocol has been designed and optimized for JUBT1. The freeze-dried strain renders the same characteristics as that of the native strain after revival. The mercury removal efficiency of the biofilm of revived freeze-dried cells is comparable to that of the native cells with a negligible deviation (absolute) of 0.16%. Thus, the present study could prove itself sufficient to throw some light on the future prospects on demercurization of wastewater using bacterial biofilm.
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Acknowledgments
The authors are grateful to UPE II, Jadavpur University, and funding by University Grant Commission, New Delhi, for the financial support. The authors are indebted to the learned reviewers of this journal for their valuable guidance to revise the manuscript.
References
Barkay, T., Miller, S. M., and Summers, A. O. (2003). “Bacterial mercury resistance from atoms to ecosystems.” FEMS Microbiol. Rev., 27(2–3), 355–384.
Bhattacharya, I., Chakraborty, R., and Chowdhury, R. (2014). “Intensification of freeze-drying rate of bacillus subtilis MTCC 2396 using tungsten halogen radiation: Optimization of moisture content and α-amylase activity.” Dry. Tech., 32(7), 801–812.
Brunke, M., et al. (1993). “Microbial retention of mercury from waste streams in a laboratory column containing merA gene bacteria.” FEMS. Microbiol. Rev., 11(1–3), 145–152.
Canstein, H. V., et al. (2002). “Spatially oscillating activity and microbial succession of mercury-reducing biofilms in a technical scale bioremediation system.” Appl. Environ. Microbiol., 68(4), 1938–1946.
Canstein, H. V., Li, Y., Felske, A., and Wagner-Dobler, I. (2001). “Long-term stability of mercury-reducing microbial biofilm communities analyzed by 16S-23S rDNA interspacer region polymorphism.” Microb. Ecol., 42(4), 624–634.
Canstein, H. V., Li, Y., Timmis, K. N., Deckwer, W. D., and Wagner-Döbler, I. (1999). “Removal of mercury from chloralkali electrolysis wastewater by a mercury-resistant Pseudomonas putida strain.” Appl. Environ. Microbiol., 65, 5279–5284.
Champier, L., Duart, V., Michaud-Soret, I., and Coves, J. (2004). “Characterization of the MerD protein from Ralstonia metallidurans CH34: A possible role in bacterial mercury resistance by switching off the induction of the Mer Operon.” Mol. Microbiol., 52(5), 1475–1485.
Chen, C. Y., and Chen, S. D. (2000). “Biofilm characteristics in biological denitrification biofilm reactors.” Wat. Sci. Technol., 41(4), 147–154.
Dash, H. R., and Das, S. (2012). “Bioremediation of mercury and the importance of bacterial mer genes.” Int. Biodeterior. Biodegrad., 75, 207–213.
De, R., and Chowdhury, R. (2013). “Hydrodynamics of a packed bed biofilm reactor (PBBR) for the removal of ion- RTD experiments with the biotic and characteristically similar abiotic films and axial dispersion model.” J. Chem. Technol. Biotechnol., 88(9), 1612–1621.
Dzairi, F. Z., et al. (2004). “Bacterial volatilization of mercury by immobilized bacteria in fixed and fluidized bed bioreactors.” Ann. Microbiol., 54(4), 353–364.
Essa, A. M. M. (2012). “The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains.” Afr. J. Microbiol. Res., 6(6), 1255–1261.
Frischmuth, A., Weppen, P., and Deckwer, W. D. (1991). “Mercury removal from aqueous media by active microbial processes.” Bio. Eng., 7, 38–47.
Ghoshal, S., Bhattacharya, P., and Chowdhury, R. (2011). “De-mercurization of wastewater by Bacillus cereus (JUBT1): Growth kinetics, biofilm reactor study and field emission scanning electron microscopic analysis.” J. Hazard. Mater., 194, 355–361.
Hamlett, N. V., Landale, E. C., Davis, B. H., and Summers, A. O. (1992). “Roles of the Tn21 merT, merP and merC gene products in mercury resistance and mercury binding.” J. Bacteriol., 174, 6377–6385.
Huang, C. C., Chien, M. F., and Lin, K. H. (2010). “Bacterial mercury resistance of TnMERI1 and its’ application in bioremediation.” Biological responses to contaminants: From molecular to community level e interdisciplinary studies on environmental chemistry, N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso, H. Iwata, and S. Tanabe, eds., Vol. 3, Terrapub, Tokyo, 23–29.
Huang, C. C., Narita, M., and Yamagata, T. (2002). “Characterization of two regulatory genes of the mercury resistance determinants from TnMERI1 by luciferase-based examination.” Gene, 301(1–2), 13–20.
Ibusquiza, P. S., Herrera, J. J. R., Vazquez-Sanchez, D., and Cabo, M. L. (2012). “Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida.” Food Control, 25(1), 202–210.
Kiyono, M., and Pan-Hou, H. (1999). “The merG gene product is involved in phenylmercury resistance in Pseudomonas strain K-62.” J. Bacteriol., 81, 726–730.
Korstgens, V., Flemming, H. C., Wingender, J., and Borchard, W. (2001). “Uniaxial compression measurement device for investigation of the mechanical stability of biofilms.” J. Microbiol. Methods, 46(1), 9–17.
Lazarova, V., and Manem, J. (2000). Innovative biofilm treatment technologies for water and wastewater treatment, in biofilms II: Process analysis and applications, J. D. Bryers, ed., Wiley-Liss, New York, 159–206.
Leonhauser, J., Wang, W., Deckwer, W. D., and Wagner-Dobler, I. (2007). “Functioning of the mercury resistance operon at extremely high Hg(II) loads in a chemostat: A proteome analysis.” J. Biotechnol., 132(4), 469–480.
Liebert, C. A., Hall, R. M., and Summers, A. O. (1999). “Transposon Tn21, flagship of the floating genome.” Microbiol. Mol. Biol. R., 63, 507–522.
Liebert, C. A., Wireman, J., Smith, T., and Summers, A. O. (1997). “Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates.” Appl. Environ. Microbiol., 63, 1066–1076.
Osborn, A. M., Bruce, K. D., Strike, P., and Ritchie, D. A. (1997). “Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon.” FEMS. Microbiol. Rev., 19(4), 239–262.
Otero, M. C., Espeche, M. C., and Nader-Macias, M. E. (2007). “Optimization of the freeze-drying media and survival throughout storage of freeze dried Lactobacillus gasseri and Lactobacillus delbrueckii sub sp. for veterinarian probiotic applications.” Process. Biochem., 42(10), 1406–1411.
Powlowski, J., and Sahlman, L. (1999). “ Reactivity of the two essential cysteine residues of the periplasmic mercuric ion-binding protein, MerP.” J. Biol. Chem., 274(47), 33320–33326.
Rossy, E., et al. (2004). “Is the cytoplasmic loop of MerT, the mercuric ion transport protein, involved in mercury transfer to the mercuric reductase?” FEBS. Lett., 575(1–3), 86–90.
Schelert, J., Dixit, V., Hoang, V., Simbahan, J., Drozda, M., and Blum, P. (2004). “Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption.” J. Bacteriol., 186(2), 427–437.
Schottel, J. L., Mandal, A., Clark, D., Silver, S., and Hedges, R. W. (1978). “The mercuric and organomercurial detoxifying enzymes from a plasmid bearing strain of Escherichia coli.” J. Biol. Chem., 253, 4341–4349.
Singh, R., Paul, D., and Jain, R. K. (2006). “Biofilms: Implications in bioremediation.” Trends. Microbiol., 14(9), 389–397.
Sone, Y., Pan-Hou, H., Nakamura, R., Sakabe, K., and Kiyono, M. (2010). “Roles played by MerE and MerT in the transport of inorganic and organic mercury compounds in Gramenegative bacteria.” J. Health. Sci., 56(1), 123–127.
Wagner-Dobler, I., Canstein, H. V., Li, Y., Timmis, K. N., and Deckwer, W. (2000a). “Removal of mercury from chemical wastewater by microorganisms in technical scale.” Environ. Sci. Technol., 34(21), 4628–4634.
Wagner-Dobler, I., Lünsdorf, H., Lübbehüsen, T., Canstein, H. F. V., and Li, Y. (2000b). “Structure and species composition of mercury reducing biofilms.” Appl. Environ. Microbiol., 66(10), 4559–4563.
Wireman, J., Liebert, C. A., Smith, T., and Summers, A. O. (1997). “ Association of mercury resistance with antibiotic resistance in the gram-negative fecal bacteria of primates.” Appl. Environ. Microb., 63, 4494–4503.
Xiao-xi, Z., Jian-xin, T., Pei, J., Hong-wei, L., Zhi-min, D., and Xue-duan, L. (2010). “Isolation, characterization and extraction of mer gene of resisting strain D2.” T. Nonferr. Met. Soc., 20(3), 507–512.
Yan, R., Yang, F., Wu, Y., Hu, Z., and Nath, B. (2011). “Cadmium and mercury removal from non-point source wastewater by a hybrid bioreactor.” Bioresour. Technol., 102(21), 9927–9932.
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© 2015 American Society of Civil Engineers.
History
Received: Sep 5, 2014
Accepted: May 5, 2015
Published online: Jul 3, 2015
Discussion open until: Dec 3, 2015
Published in print: Sep 1, 2016
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