Case Studies
Jul 3, 2015

Biofilm Reactor for Hg2+ Removal: Review with Challenges and A Study with Freeze Dried Bacteria

Publication: Journal of Environmental Engineering
Volume 142, Issue 9

Abstract

The present article intends to make a state-of-the review of removal of Hg2+ ions from wastewater using biofilm reactor. Preservation of mercury resistant bacteria has been identified as one of the challenges regarding commercialization of biofilm reactors for this purpose. Finally, a case study on biofilm reactor using freeze-dried Bacillus cereus (JUBT1) has been incorporated. A novel, cost-effective, and economically feasible freeze-drying protocol has been designed and optimized for JUBT1. The freeze-dried strain renders the same characteristics as that of the native strain after revival. The mercury removal efficiency of the biofilm of revived freeze-dried cells is comparable to that of the native cells with a negligible deviation (absolute) of 0.16%. Thus, the present study could prove itself sufficient to throw some light on the future prospects on demercurization of wastewater using bacterial biofilm.

Get full access to this article

View all available purchase options and get full access to this article.

Acknowledgments

The authors are grateful to UPE II, Jadavpur University, and funding by University Grant Commission, New Delhi, for the financial support. The authors are indebted to the learned reviewers of this journal for their valuable guidance to revise the manuscript.

References

Barkay, T., Miller, S. M., and Summers, A. O. (2003). “Bacterial mercury resistance from atoms to ecosystems.” FEMS Microbiol. Rev., 27(2–3), 355–384.
Bhattacharya, I., Chakraborty, R., and Chowdhury, R. (2014). “Intensification of freeze-drying rate of bacillus subtilis MTCC 2396 using tungsten halogen radiation: Optimization of moisture content and α-amylase activity.” Dry. Tech., 32(7), 801–812.
Brunke, M., et al. (1993). “Microbial retention of mercury from waste streams in a laboratory column containing merA gene bacteria.” FEMS. Microbiol. Rev., 11(1–3), 145–152.
Canstein, H. V., et al. (2002). “Spatially oscillating activity and microbial succession of mercury-reducing biofilms in a technical scale bioremediation system.” Appl. Environ. Microbiol., 68(4), 1938–1946.
Canstein, H. V., Li, Y., Felske, A., and Wagner-Dobler, I. (2001). “Long-term stability of mercury-reducing microbial biofilm communities analyzed by 16S-23S rDNA interspacer region polymorphism.” Microb. Ecol., 42(4), 624–634.
Canstein, H. V., Li, Y., Timmis, K. N., Deckwer, W. D., and Wagner-Döbler, I. (1999). “Removal of mercury from chloralkali electrolysis wastewater by a mercury-resistant Pseudomonas putida strain.” Appl. Environ. Microbiol., 65, 5279–5284.
Champier, L., Duart, V., Michaud-Soret, I., and Coves, J. (2004). “Characterization of the MerD protein from Ralstonia metallidurans CH34: A possible role in bacterial mercury resistance by switching off the induction of the Mer Operon.” Mol. Microbiol., 52(5), 1475–1485.
Chen, C. Y., and Chen, S. D. (2000). “Biofilm characteristics in biological denitrification biofilm reactors.” Wat. Sci. Technol., 41(4), 147–154.
Dash, H. R., and Das, S. (2012). “Bioremediation of mercury and the importance of bacterial mer genes.” Int. Biodeterior. Biodegrad., 75, 207–213.
De, R., and Chowdhury, R. (2013). “Hydrodynamics of a packed bed biofilm reactor (PBBR) for the removal of Hg2+ ion- RTD experiments with the biotic and characteristically similar abiotic films and axial dispersion model.” J. Chem. Technol. Biotechnol., 88(9), 1612–1621.
Dzairi, F. Z., et al. (2004). “Bacterial volatilization of mercury by immobilized bacteria in fixed and fluidized bed bioreactors.” Ann. Microbiol., 54(4), 353–364.
Essa, A. M. M. (2012). “The effect of a continuous mercury stress on mercury reducing community of some characterized bacterial strains.” Afr. J. Microbiol. Res., 6(6), 1255–1261.
Frischmuth, A., Weppen, P., and Deckwer, W. D. (1991). “Mercury removal from aqueous media by active microbial processes.” Bio. Eng., 7, 38–47.
Ghoshal, S., Bhattacharya, P., and Chowdhury, R. (2011). “De-mercurization of wastewater by Bacillus cereus (JUBT1): Growth kinetics, biofilm reactor study and field emission scanning electron microscopic analysis.” J. Hazard. Mater., 194, 355–361.
Hamlett, N. V., Landale, E. C., Davis, B. H., and Summers, A. O. (1992). “Roles of the Tn21 merT, merP and merC gene products in mercury resistance and mercury binding.” J. Bacteriol., 174, 6377–6385.
Huang, C. C., Chien, M. F., and Lin, K. H. (2010). “Bacterial mercury resistance of TnMERI1 and its’ application in bioremediation.” Biological responses to contaminants: From molecular to community level e interdisciplinary studies on environmental chemistry, N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso, H. Iwata, and S. Tanabe, eds., Vol. 3, Terrapub, Tokyo, 23–29.
Huang, C. C., Narita, M., and Yamagata, T. (2002). “Characterization of two regulatory genes of the mercury resistance determinants from TnMERI1 by luciferase-based examination.” Gene, 301(1–2), 13–20.
Ibusquiza, P. S., Herrera, J. J. R., Vazquez-Sanchez, D., and Cabo, M. L. (2012). “Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida.” Food Control, 25(1), 202–210.
Kiyono, M., and Pan-Hou, H. (1999). “The merG gene product is involved in phenylmercury resistance in Pseudomonas strain K-62.” J. Bacteriol., 81, 726–730.
Korstgens, V., Flemming, H. C., Wingender, J., and Borchard, W. (2001). “Uniaxial compression measurement device for investigation of the mechanical stability of biofilms.” J. Microbiol. Methods, 46(1), 9–17.
Lazarova, V., and Manem, J. (2000). Innovative biofilm treatment technologies for water and wastewater treatment, in biofilms II: Process analysis and applications, J. D. Bryers, ed., Wiley-Liss, New York, 159–206.
Leonhauser, J., Wang, W., Deckwer, W. D., and Wagner-Dobler, I. (2007). “Functioning of the mercury resistance operon at extremely high Hg(II) loads in a chemostat: A proteome analysis.” J. Biotechnol., 132(4), 469–480.
Liebert, C. A., Hall, R. M., and Summers, A. O. (1999). “Transposon Tn21, flagship of the floating genome.” Microbiol. Mol. Biol. R., 63, 507–522.
Liebert, C. A., Wireman, J., Smith, T., and Summers, A. O. (1997). “Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates.” Appl. Environ. Microbiol., 63, 1066–1076.
Osborn, A. M., Bruce, K. D., Strike, P., and Ritchie, D. A. (1997). “Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon.” FEMS. Microbiol. Rev., 19(4), 239–262.
Otero, M. C., Espeche, M. C., and Nader-Macias, M. E. (2007). “Optimization of the freeze-drying media and survival throughout storage of freeze dried Lactobacillus gasseri and Lactobacillus delbrueckii sub sp. for veterinarian probiotic applications.” Process. Biochem., 42(10), 1406–1411.
Powlowski, J., and Sahlman, L. (1999). “ Reactivity of the two essential cysteine residues of the periplasmic mercuric ion-binding protein, MerP.” J. Biol. Chem., 274(47), 33320–33326.
Rossy, E., et al. (2004). “Is the cytoplasmic loop of MerT, the mercuric ion transport protein, involved in mercury transfer to the mercuric reductase?” FEBS. Lett., 575(1–3), 86–90.
Schelert, J., Dixit, V., Hoang, V., Simbahan, J., Drozda, M., and Blum, P. (2004). “Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption.” J. Bacteriol., 186(2), 427–437.
Schottel, J. L., Mandal, A., Clark, D., Silver, S., and Hedges, R. W. (1978). “The mercuric and organomercurial detoxifying enzymes from a plasmid bearing strain of Escherichia coli.” J. Biol. Chem., 253, 4341–4349.
Singh, R., Paul, D., and Jain, R. K. (2006). “Biofilms: Implications in bioremediation.” Trends. Microbiol., 14(9), 389–397.
Sone, Y., Pan-Hou, H., Nakamura, R., Sakabe, K., and Kiyono, M. (2010). “Roles played by MerE and MerT in the transport of inorganic and organic mercury compounds in Gramenegative bacteria.” J. Health. Sci., 56(1), 123–127.
Wagner-Dobler, I., Canstein, H. V., Li, Y., Timmis, K. N., and Deckwer, W. (2000a). “Removal of mercury from chemical wastewater by microorganisms in technical scale.” Environ. Sci. Technol., 34(21), 4628–4634.
Wagner-Dobler, I., Lünsdorf, H., Lübbehüsen, T., Canstein, H. F. V., and Li, Y. (2000b). “Structure and species composition of mercury reducing biofilms.” Appl. Environ. Microbiol., 66(10), 4559–4563.
Wireman, J., Liebert, C. A., Smith, T., and Summers, A. O. (1997). “ Association of mercury resistance with antibiotic resistance in the gram-negative fecal bacteria of primates.” Appl. Environ. Microb., 63, 4494–4503.
Xiao-xi, Z., Jian-xin, T., Pei, J., Hong-wei, L., Zhi-min, D., and Xue-duan, L. (2010). “Isolation, characterization and extraction of mer gene of Hg2+ resisting strain D2.” T. Nonferr. Met. Soc., 20(3), 507–512.
Yan, R., Yang, F., Wu, Y., Hu, Z., and Nath, B. (2011). “Cadmium and mercury removal from non-point source wastewater by a hybrid bioreactor.” Bioresour. Technol., 102(21), 9927–9932.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 142Issue 9September 2016

History

Received: Sep 5, 2014
Accepted: May 5, 2015
Published online: Jul 3, 2015
Discussion open until: Dec 3, 2015
Published in print: Sep 1, 2016

Permissions

Request permissions for this article.

Authors

Affiliations

Ishita Bhattacharya
Research Fellow, Dept. of Chemical Engineering, Jadavpur Univ., Kolkata 700032, India.
Rajat Chakraborty
Associate Professor, Dept. of Chemical Engineering, Jadavpur Univ., Kolkata 700032, India.
Ranjana Chowdhury [email protected]
Professor, Dept. of Chemical Engineering, Jadavpur Univ., Kolkata 700032, India (corresponding author). E-mail: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share