Two-Phase Biodegradation of Phenol in a Hollow Fiber Membrane Bioreactor
Publication: Journal of Environmental Engineering
Volume 139, Issue 5
Abstract
A hollow fiber membrane bioreactor (HFMB) was employed for aqueous-organic two-phase biodegradation of phenol using Pseudomonas putida ATCC 11172, and the results were compared with that in a two-phase partitioning bioreactor (TPPB). Phenol containing 2-undecanone was nondispersively contacted with mineral medium that was inoculated with the bacteria. P. putida in suspension was able to biodegrade inhibitory phenol concentrations at without experiencing severe substrate inhibition. For example, phenol was completely biodegraded in 46 h at a maximum specific growth rate of , whereas the biomass yield and average biodegradation rate were and , respectively. Biomass yield and maximum specific growth rate decreased concomitantly with increasing phenol concentration. Phenol removal started at an exponential rate, and subsequently attained a linear profile in nutrient-limited conditions. Unlike conventional two-phase biodegradation systems, HFMB offered a better growth environment for the cells, as evident from the absence of the lag phase. The HFMB was more environmentally friendly and offered ease of operation and analysis that could mitigate the problems of foaming and emulsification that are associated with conventional TPPBs. In evaluating the effects of membrane area on biodegradation rate, the writers found that by doubling the interfacial area, phenol could be mineralized within 43 h with improved growth and removal rates. For comparable mass transfer flux across the aqueous-organic interface, biodegradation was faster in the HFMB relative to that in TPPB.
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Information
Published In
Journal of Environmental Engineering
Volume 139 • Issue 5 • May 2013
Pages: 654 - 660
Copyright
© 2013 American Society of Civil Engineers.
History
Received: Apr 18, 2012
Accepted: Nov 7, 2012
Published online: Nov 9, 2012
Published in print: May 1, 2013
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