Integrated Chemical-Biological Remediation for Polycyclic Aromatic Hydrocarbons Contaminated Soil
Publication: Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 8, Issue 2
Abstract
An integrated slurry-phase remedial system was developed for cleanup of polycyclic aromatic hydrocarbon (PAH) contaminated soil in Singapore. The bench-scale remedial system consisted of a chemical treatment to initiate the degradation of PAHs followed by a biological treatment. The 20% (weight/volume) slurry of PAH contaminated soil was mixed continuously at 180 rpm in the 1 L bioreactor. PAH contaminated soil was synthesized by spiking naphthalene, phenanthrene, and pyrene onto uncontaminated model soil. Fenton’s reagent was added during chemical treatment to initiate degradation of PAHs. The removal efficiency of chemical treatment was 76%. The active PAH-degrading strain of bacteria was isolated from a slurry-phase reactor, where garden soil was used as a source of indigenous microorganisms. The sequencing of 16S rDNA showed that this bacterial strain was close to Pseudomonas beteli (identities=99%, 553 from 556 position). The application of isolated bacteria for bioremediation of PAH contaminated soil resulted in a 96–97% removal of PAHs. It was possible to use the cultivated PAH-degrading microorganisms as an effective inoculum to start up the remediation process. The proposed integrated chemical-biological remedial system was capable of treating PAH contaminated soil efficiently in terms of high-ring PAH degradation, less expensive process, and short treatment time required.
Get full access to this article
View all available purchase options and get full access to this article.
References
Agency for Toxic Substances and Disease Registry (ATSDR). (1995). Toxicological profile for hydrocarbons (PAHs), U.S. Public Health Service, Atlanta.
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J.(1997). “Gapped BLAST and PSI-blast: A new generation of protein database search programs.” Nucleic Acids Res., 25(17), 3389–3402.
Castaldi, F. J. (1993). “Slurry bioremediation of polycyclic aromatic hydrocarbons in soil wash concentrates.” Applied biotechnology for site remediation, R. E. Hinchee, D. B. Anderson, F. B. Metting, Jr., and G. D. Sayles, eds., Lewis, Boca Raton, Fla., 99–108.
Dabestani, R., and Ivanov, I. N.(1999). “Invited review: A compilation of physical, spectroscopic and photophysical properties of polycyclic aromatic hydrocarbons.” Photochem. Photobiol., 70(1), 10–34.
Dean, D., and Cerniglia, C. E.(1996). “Degradation of pyrene by Mycobacterium flavescens.” Appl. Microbiol. Biotechnol., 46, 307–312.
Dong, M. W., Duggan, J. X., and Stefanou, S.(1993). “A quick turnaround HPLC method for polynuclear aromatic hydrocarbons (PAHs) in soil, water, and waste oil.” LCGC Magazine, 11(11), 802–823.
Guha, S., Peters, C. A., and Jaffé, P. R.(1999). “Multisubstrate biodegradation kinetics of naphthalene, phenanthrene and pyrene mixtures.” J. Biosci. Bioeng., 65(5), 491–499.
Henner, P., Schiavon, M., Druelle, V., and Lichtfouse, E.(1999). “Phytotoxicity of ancient gaswork soils. Effect of polycyclic aromatic hydrocarbons (PAHs) on plant germination.” Org. Geochem., 30, 963–969.
Hwang, E. Y. (1999). “Bioremediation of diesel-contaminated soil using simulated composting process.” PhD thesis, Konkuk Univ., Korea.
Juhasz, A. L., Britz, M. L., and Stanley, G. A.(1997). “Degradation of benzo[a]pyrene, dibenz[a,h]anthracene and coronene by Burkholderia cepacia.” Water Sci. Technol., 36(10), 45–51.
Pradhan, S. P., Paterek, J. R., Liu, B. Y., Conrad, J. R., and Srivastava, V. J.(1997). “Pilot-scale bioremediation of PAH-contaminated soils.” Appl. Biochem. Biotechnol., 63–65, 759–773.
Rulkens, W. H., Tichy, R., and Grotenhuis, J. T. C.(1998). “Remediation of polluted soil and sediment: Perspectives and failures.” Water Sci. Technol., 37(8), 27–35.
Stabnikova, E. V., Selezneva, M. V., Dul’gerov, A. N., and Ivanov, V. N.(1996). “Use of the biological preparation Lestan for the soil bioremediation.” Appl. Biochem. Microbiol., 32(2), 202–206.
Wang, J. Y., Stabnikova, O., Tay, S. T. L., Ivanov, V., and Tay, J. H.(2003). “Intensive bioconversion of sewage sludge and food waste by Bacillus thermoamylovorans.” World J. Microb. Biot., 19(4), 427–432.
Wang, S. Y., and Vipulanandan, C.(2001). “Biodegradation of naphthalene-contaminated soils in slurry bioreactors.” J. Environ. Eng., 127(8), 748–754.
Woo, S. H., and Park, J. M.(1999). “Evaluation of drum bioreactor performance used for decontamination of soil polluted with polycyclic aromatic hydrocarbons.” J. Chem. Technol. Biotechnol., 74, 937–944.
Zeng, Y., Hong, P. K. A., and Wavrek, D. A.(2000). “Integrated chemical-biological treatment of benzopyrene.” Environ. Sci. Technol., 34(5), 854–862.
Information & Authors
Information
Published In
Copyright
Copyright © 2004 American Society of Civil Engineers.
History
Received: Mar 13, 2003
Accepted: Mar 13, 2003
Published online: Mar 15, 2004
Published in print: Apr 2004
Authors
Metrics & Citations
Metrics
Citations
Download citation
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.