Biotransformation of Trichloroethylene by a Phenol-Induced Mixed Culture
Publication: Journal of Environmental Engineering
Volume 122, Issue 7
Abstract
Biodegradation of trichloroethylene (TCE) was studied using a mixed culture of aerobic, phenol-induced organisms. Abiotic experiments showed that sorption of TCE to biomass was negligible in the systems studied. The effects of influent phenol and TCE concentration on the TCE degradation capacity of the culture were studied using chemostats. A relationship exists between the influent phenol/TCE ratio and TCE biodegradation. TCE transformation yields ranged from 0.052 to 0.222 mg TCE removed/mg phenol removed. Monod kinetic coefficients for phenol degradation were determined. Monod kinetic coefficients were also determined for TCE biotransformation by resting cells. The concept of transformation capacity was used to model the decrease in active biomass concentration caused by TCE transformation. In mineralization studies using 14 C-labeled TCE, 22% of the degraded mass of TCE was transformed to carbon dioxide, 8.8% was incorporated into biomass, 42% was transformed to nonvolatile products, with the remaining, unrecovered 27% most likely transformed into volatile or semivolatile products.
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References
1.
Alvarez-Cohen, L., and McCarty, P. L.(1991a). “Effects of toxicity, aeration, and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture.”Appl. Envir. Microbiol., 57(1), 228–235.
2.
Alvarez-Cohen, L., and McCarty, P. L.(1991b). “Two-stage dispersed-growth treatment of halogenated aliphatic compounds by cometabolism.”Envir. Sci. & Technol., 25(8), 1387–1393.
3.
American Public Health Association. (1990). Standard methods for the examination of water and wastewater . APHA, New York, N.Y.
4.
Bielefeldt, A. R., Stensel, H. D., and Strand, S. E. (1995). “Degradation of chlorinated aliphatic compounds by methane and phenol-oxidizing bacteria: Intermediate toxicity and competitive inhibition.”Bioremediation of chlorinated solvents, R. E. Hinchee, A. Leeson, and L. Semprini, eds., Battelle Press, Columbus, Ohio, 237–244.
5.
Budavari, S. (1989). The Merck Index . Merck and Co., Inc., Rahway, N.J.
6.
Chang, H-L., and Alvarez-Cohen, L.(1995). “Transformation capacities of chlorinated organics by mixed cultures enriched on methane, propane, toluene, or phenol.”Biotechnol. and Bioengrg., 45(5), 440–449.
7.
Cothern, C. R., Coniglio, W. A., and Marcus, W. L.(1986). “Estimating risk to human health.”Envir. Sci. Technol., 20(1), 111–116.
8.
Coyle, C. G., Parkin, G. F., and Gibson, D. T.(1993). “Aerobic, phenol-induced TCE degradation in completely mixed, continuous culture reactors.”Biodegradation, 4(1), 59–69.
9.
Criddle, C. S.(1993). “The kinetics of cometabolism.”Biotechnol. and Bioengrg., 41(11), 1048–1056.
10.
Fan, A. M. (1988). “Trichloroethylene: Water contamination and health risk assessment.”Reviews of Environmental Contamination and Toxicology, Vol. 101, G. W. Ware, ed., Springer-Verlag, New York, N.Y., 55–92.
11.
Folsom, B. R., Chapman, P. J., and Pritchard, P. H. (1990). “Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: Kinetics and interactions between substrates.”Appl. Envir. Microbiol., 56(5), 1279–1285.
12.
Folsom, B. R., and Chapman, P. J. (1991). “Performance Characterization of a model bioreactor for the biodegradation of trichloroethylene by Pseudomonas cepacia C4.”Appl. Envir. Microbiol., 57(6), 1602–1608.
13.
Gossett, J. M. (1987). “Measurement of Henry's Law Constants for C 1 and C 2 chlorinated hydrocarbons.”Envir. Sci. Technol., 21(2), 202–208.
14.
Havlicek, L. L., and Crain, R. D. (1988). Practical statistics for the physical sciences . American Chemical Society, Washington, D.C.
15.
Henry, S. M., and Grbic-Galic, D.(1991). “Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer.”Appl. Envir. Microbiol., 57(1), 236–244.
16.
Hopkins, G. D., Semprini, L., and McCarty, P. L.(1993a). “Microcosm and in-situ field studies of enhanced biotransformation of trichloroethylene by phenol-utilizing microorganisms.”Appl. Envir. Microbiol., 59(7), 2277–2285.
17.
Hopkins, G. D., Munakata, J., Semprini, L., and McCarty, P. L.(1993b). “Trichloroethylene concentration effects on pilot field-scale in-situ groundwater bioremediation by phenol-oxidizing microorganisms.”Envir. Sci. Technol., 27(12), 2542–2547.
18.
Landa, A. S., Sipkema, E. M., Weijma, J., Beenackers, A. A. C. M., Dolfing, J., and Janssen, D. B. (1994). “Cometabolic degradation of trichloroethylene by Pseudomonas cepacia G4 in a chemostat with toluene as the primary substrate.”Appl. Envir. Microbiol., 60(9), 3368–3374.
19.
Maltoni, C., and Lefemine, G.(1974). “Carcinogenicity bioassays of vinyl chloride. I. research plan and early results.”Envir. Res., 7(3), 387–405.
20.
Martin, R. W.(1949). “Rapid colorimetric estimation of phenol.”Analytical Chem., 21(11), 1419–1420.
21.
McFarland, M. J., Vogel, C. M., and Spain, J. C.(1992). “Methanotrophic cometabolism of trichloroethylene in a two stage bioreactor system.”Water Res., 26(2), 259–265.
22.
Metcalf and Eddy, Inc. (1990). Wastewater engineering: Treatment, disposal, and reuse . McGraw-Hill, Inc., New York, N.Y.
23.
Nelson, M. J. K., Montgomery, S. O., Mahaffey, W. R., and Pritchard, P. H.(1987). “Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway.”Appl. Envir. Microbiol., 53(5), 949–954.
24.
Nelson, M. J. K., Montgomery, S. O., O'Neill, E. J., and Pritchard, P. H.(1986). “Aerobic metabolism of trichloroethylene by a bacterial isolate.”Appl. Envir. Microbiol., 52(2), 383–384.
25.
Ong, S. K., and Bowers, A. R.(1990). “Steady-state analysis for biological treatment of inhibitory substrates.”J. Envir. Engrg., ASCE, 116(6), 1013–1028.
26.
Rasche, M. E., Hyman, M. R., and Arp, D. J. (1991). “Factors limiting aliphatic chlorocarbon degradation by Nitrosomonas europaea : Cometabolic inactivation of ammonia monooxygenase and substrate specificity.”Appl. Envir. Microbiol., 57(10), 2986–2994.
27.
Reklaitis, G. V., Ravindran, A., and Ragsdell, K. M. (1983). Engineering optimization: Methods and applications . John Wiley and Sons, Inc., New York, N.Y.
28.
Rittmann, B. E., Valocchi, A. J., Odencrantz, J. E., and Bae, W. (1988). “In-situ bioreclamation of contaminated groundwater.”UILU-WRC-88-209, Res. Rep. 209, Water Resour. Ctr., Univ. of Illinois at Urbana-Champaign, Urbana-Champaign, Ill.
29.
Schmidt, S. K., Simkins, S., and Alexander, M.(1985). “Models for the kinetics of biodegradation of organic compounds not supporting growth.”Appl. Envir. Microbiol., 50(4), 323–331.
30.
Simkins, S., and Alexander, M.(1984). “Models for mineralization kinetics with the variables of substrate concentration and population density.”Appl. Envir. Microbiol., 47(6), 1299–1306.
31.
Speitel, Jr., and G. E., and Leonard(1992). “A sequencing biofilm reactor for the treatment of chlorinated solvents using methanotrophs.”Water Envir. Res., 64(2), 712–719.
32.
Storck, W.(1987). “Chlorinated solvent use hurt by federal rules.”Chem. Engrg. News, 65(39), 11.
33.
Strandberg, G. W., Donaldson, T. L., and Farr, L. L.(1989). “Degradation of trichloroethylene and trans-1,2-dichloroethylene by a methanotrophic consortium in a fixed-film, packed-bed bioreactor.”Envir. Sci. Technol., 23(11), 1422–1425.
34.
Tsien, H. C., Brusseau, G. A., Hanson, R. S., and Wackett, L. P. (1989). “Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.”Appl. Envir. Microbiol., 55(12), 3155–3161.
35.
Vanelli, T., Logan, M., Arcerio, D. M., and Hooper, A. B. (1990). “Degradation of halogenated aliphatic compounds by the ammonia-oxidizing bacterium Nitrosomonas europaea .”Appl. Envir. Microbiol., 56(4), 1169–1171.
36.
Vogel, T. M., and McCarty, P. L.(1985). “Biotransformation of tetrachloroethylene to trichloroethylene, vinyl chloride, and carbon dioxide under methanogenic systems.”Appl. Envir. Microbiol., 49(5), 1080–1083.
37.
Wackett, L. P., and Householder, S. R. (1989). “Toxicity of trichloroethylene to Pseudomonas putida F1 is mediated by toluene dioxygenase.”Appl. Envir. Microbiol., 55(10), 2723–2725.
38.
Wackett, L. P., Brusseau, G. A., Householder, S. R., and Hanson, R. S.(1989). “Survey of microbial oxygenases: Trichloroethylene degradation by propane-oxidizing bacteria.”Appl. Envir. Microbiol., 55(11), 2960–2964.
39.
Wackett, L. P., and Gibson, D. T. (1988). “Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1.”Appl. Envir. Microbiol., 54(7), 1703–1708.
40.
Westrick, J. J., Mello, J. W., and Thomas, R. F.(1984). “The groundwater supply survey.”J. Am. Water Works Assoc., 75(5), 52–59.
41.
Winter, R. B., Yen, K. M., and Ensley, B. D. (1989). “Efficient degradation of trichloroethylene by a recombinant Escherechia coli .”Bio/Technol., 7(3), 282–285.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 122 • Issue 7 • July 1996
Pages: 581 - 589
Copyright
Copyright © 1996 American Society of Civil Engineers.
History
Published online: Jul 1, 1996
Published in print: Jul 1996
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