Exploring the Effects of Different Methane and Oxygen Concentrations on the Methane-Oxidizing Bacteria Mixed Community
Publication: Journal of Environmental Engineering
Volume 149, Issue 12
Abstract
Drainage-based gas control methods will cause mine gas to be discharged into the atmosphere, causing environmental pollution and aggravating the greenhouse effect. Methane in mine gas can be oxidatively decomposed by using microbial technology, to reduce mine gas emissions and the threat of gas to mine safety. However, coal mines’ methane and oxygen concentrations change in real time. To explore the effects of different methane and oxygen concentrations on the methane-oxidizing bacteria mixed community, a methane-oxidizing bacteria mixed community was enriched and cultured from mine soil samples in this study, and its community structure, cell morphology, and methane degradation ability were analyzed. The results showed that the methane-oxidizing bacteria Methylomicrobium accounted for 55.6% of the total, Cloacibacterium accounted for 19.39%, and Methylophilus accounted for 10.3%. The methane degradation rate of the mixed community can reach . Using the Michaelis–Menten kinetic equation analysis, it was found that the methane degradation effect was the highest when the methane concentration was 1%–20%, and the maximum methane oxidation rate could reach . When the oxygen concentration ranged from 1% to 50%, the methane degradation ability of methanotrophs gradually increased with the increase of oxygen concentration.
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Data Availability Statement
All data, models, and code generated or used during the study appear in the published article.
Acknowledgments
This research was jointly supported by the National Natural Science Foundation of China (51674157).
Author contributions: Jun Xie: investigation, visualization, and writing-review and editing. Xiangke Sun: investigation, writing-original draft, and writing-review and editing. Haigang Du: investigation, and writing-review and editing. Dawei Chen: investigation and resources. Yi Wang: investigation and resources.
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Published In
Journal of Environmental Engineering
Volume 149 • Issue 12 • December 2023
Copyright
© 2023 American Society of Civil Engineers.
History
Received: Feb 23, 2023
Accepted: Jun 18, 2023
Published online: Sep 19, 2023
Published in print: Dec 1, 2023
Discussion open until: Feb 19, 2024
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