Optimization of Long-Chain Fatty Acid Synthesis from CO2 Using Response Surface Methodology
Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 27, Issue 4
Abstract
A microbial electrosynthesis cell (MES) is an electrochemical technique in which electrolithoautotrophic electroactive microbes fix carbon dioxide (CO2) to longer-chain volatile fatty acids (VFAs). The synthesis of VFAs from CO2 requires optimization to improve the MES performance for industrial feasibility. This work studied the effect of different parameters, such as pH, headspace gas pressure, ethanol concentration, electrolyte, and trace element concentrations, on VFA synthesis from CO2 that used mixed anaerobic consortia in serum bottles. The operational parameters were varied according to a central composite design (CCD) and response surface methodology (RSM). A global optimum for the response variables was determined. The optimum values of different operating factors to maximize VFA production that was obtained from the optimizations were 1.12 × 105 Pa pressure, pH 7.149, ethanol = 2,318.7 mg/L, three times the standard Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) 300 electrolyte concentration and five times the standard DSMZ 300 trace elements concentration. The experimental study was validated in triplicate, which considered the optimal values. The rate of total VFA production that was obtained from the operational parameters experimental study was 43.7 ± 5.9 mg/L/day under this optimized condition, which agreed with the predicted value of 30.39 mg/L/day. For the media optimization, validation of the experimental study was conducted at the optimal values, and their experimental response was 79.75 ± 19.26 mg/L/day, and the predicted response was 75.89 mg/L/day for total VFA production rates.
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Acknowledgments
The authors are grateful to Science and Engineering Research Board), a Government of India funded project (Grant Reference, SRG/2019/000757). In addition, the authors would like to thank the Department of Civil Engineering (IIT Hyderabad) for their financial support and laboratory provided for this project.
References
Ammam, F., P. L. Tremblay, D. M. Lizak, and T. Zhang. 2016. “Effect of tungstate on acetate and ethanol production by the electrosynthetic bacterium Sporomusa ovata.” Biotechnol. Biofuels 9 (1): 1–10. https://doi.org/10.1186/s13068-016-0576-0.
Annie Modestra, J., B. Navaneeth, and S. Venkata Mohan. 2015. “Bio-electrocatalytic reduction of CO2: Enrichment of homoacetogens and pH optimization towards enhancement of carboxylic acids biosynthesis.” J. CO2 Util. 10: 78–87. https://doi.org/10.1016/j.jcou.2015.04.001.
APHA, AWWA, WEF. 1998. “Standard methods for the examination of water and wastewater. 20th ed. Washington, DC: APHA, AWWA, WEF.
Bajracharya, S., A. Ter Heijne, X. Dominguez Benetton, K. Vanbroekhoven, C. J. N. Buisman, D. P. B. T. B. Strik, and D. Pant. 2015. “Carbon dioxide reduction by mixed and pure cultures in microbial electrosynthesis using an assembly of graphite felt and stainless steel as a cathode.” Bioresour. Technol. 195: 14–24. https://doi.org/10.1016/j.biortech.2015.05.081.
Cavalcante, W. de A., R. C. Leitão, T. A. Gehring, L. T. Angenent, and S. T. Santaella. 2017. “Anaerobic fermentation for n-caproic acid production: A review.” Process Biochem. 54: 106–119. https://doi.org/10.1016/j.procbio.2016.12.024.
Chaitanya, N. K., A. Tripathi, and P. Chatterjee. 2021. “Microbial electrosynthesis: Recovery of high-value volatile fatty acids from CO2.” Chap. 6 in Delivering low-carbon biofuels with bioproduct recovery, edited by L. Singh and D. M. Mahapatra, 123–142. Amsterdam, Netherlands: Elsevier.
Chaitanya, N. K., A. Rajpurohit, P. S. Nair, and P. Chatterjee. 2023. “Electrochemical synthesis of propionic acid from reduction of ethanol and carbon dioxide at various applied potentials.” Biochem. Eng. J. 194: 108896. https://doi.org/10.1016/j.bej.2023.108896.
Chen, Y., X. Li, X. Zheng, and D. Wang. 2013. “Enhancement of propionic acid fraction in volatile fatty acids produced from sludge fermentation by the use of food waste and Propionibacterium acidipropionici.” Water Res. 47 (2): 615–622. https://doi.org/10.1016/j.watres.2012.10.035.
Chong, M., N. A. Abdul Rahman, R. A. Rahim, S. A. Aziz, Y. Shirai, and M. A. Hassan. 2009. “Optimization of biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent using response surface methodology.” Int. J. Hydrog. Energy 34 (17): 7475–7482. https://doi.org/10.1016/j.ijhydene.2009.05.088.
Christodoulou, X., T. Okoroafor, S. Parry, and S. B. Velasquez-Orta. 2017. “The use of carbon dioxide in microbial electrosynthesis: Advancements, sustainability and economic feasibility.” J. CO2 Util. 18: 390–399. https://doi.org/10.1016/j.jcou.2017.01.027.
Dai, K., J. L. Wen, F. Zhang, and R. J. Zeng. 2017. “Valuable biochemical production in mixed culture fermentation: Fundamentals and process coupling.” Appl. Microbiol. Biotechnol. 101 (17): 6575–6586. https://doi.org/10.1007/s00253-017-8441-z.
Drake, H. L., A. S. Gößner, and S. L. Daniel. 2008. “Old acetogens, new light.” Ann. N.Y. Acad. Sci. 1125: 100–128. https://doi.org/10.1196/annals.1419.016.
Friedlingstein, P., M. W. Jones, M. O. Sullivan, R. M. Andrew, D. C. E. Bakker, J. Hauck, C. Le Quéré, G. P. Peters, and W. Peters. 2022. “Global carbon budget 2021.” Earth Syst. Sci. Data 14 (4): 1917–2005.
Gao, J., H. K. Atiyeh, J. R. Phillips, M. R. Wilkins, and R. L. Huhnke. 2013. “Development of low cost medium for ethanol production from syngas by Clostridium ragsdalei.” Bioresour. Technol. 147: 508–515. https://doi.org/10.1016/j.biortech.2013.08.075.
Hong, C., and W. Haiyun. 2010. “Optimization of volatile fatty acid production with co-substrate of food wastes and dewatered excess sludge using response surface methodology.” Bioresour. Technol. 101 (14): 5487–5493. https://doi.org/10.1016/j.biortech.2010.02.013.
Ismail, F., A. Rahman, S. Abd-Aziz, C. Mei Ling, and M. Ali Hassan. 2009. “Statistical optimization of biohydrogen production using food waste under thermophilic conditions.” Open Renew. Energy J. 2 (1): 124–131. https://doi.org/10.2174/1876387100902010124.
Izadi, P., J.-M. Fontmorin, B. Virdis, I. M. Head, and E. H. Yu. 2020. “The effect of the polarised cathode, formate and ethanol on chain elongation of acetate in microbial electrosynthesis.” Appl. Energy 283: 116310. https://doi.org/10.1016/j.apenergy.2020.116310.
Kannaiah Goud, R., O. Sarkar, and S. Venkata Mohan. 2014. “Regulation of biohydrogen production by heat-shock pretreatment facilitates selective enrichment of Clostridium sp.” Int. J. Hydrogen Energy 39 (14): 7572–7586. https://doi.org/10.1016/j.ijhydene.2013.10.046.
LaBelle, E. V., C. W. Marshall, J. A. Gilbert, and H. D. May. 2014. “Influence of acidic pH on hydrogen and acetate production by an electrosynthetic microbiome.” PLoS One 9 (10): 1–10. https://doi.org/10.1371/journal.pone.0109935.
Mohanakrishna, G., J. S. Seelam, K. Vanbroekhoven, and D. Pant. 2015. “An enriched electroactive homoacetogenic biocathode for the microbial electrosynthesis of acetate through carbon dioxide reduction.” Faraday Discuss. 183: 445–462. https://doi.org/10.1039/c5fd00041f.
Müller, V. 2003. “Energy conservation in acetogenic bacteria.” Appl. Environ. Microbiol. 69 (11): 6345–6353. https://doi.org/10.1128/AEM.69.11.6345-6353.2003.
Saxena, J., and R. S. Tanner. 2012. “Optimization of a corn steep medium for production of ethanol from synthesis gas fermentation by Clostridium ragsdalei.” World J. Microbiol. Biotechnol. 28 (4): 1553–1561. https://doi.org/10.1007/s11274-011-0959-0.
Steinbusch, K. J. J., H. V. M. Hamelers, and C. J. N. Buisman. 2008. “Alcohol production through volatile fatty acids reduction with hydrogen as electron donor by mixed cultures.” Water Res. 42 (15): 4059–4066. https://doi.org/10.1016/j.watres.2008.05.032.
Stephen, J. L., and B. Periyasamy. 2018. “Innovative developments in biofuels production from organic waste materials: A review.” Fuel 214 (November 2017): 623–633. https://doi.org/10.1016/j.fuel.2017.11.042.
Uçkun Kiran, E., A. P. Trzcinski, and Y. Liu. 2015. “Platform chemical production from food wastes using a biorefinery concept.” J. Chem. Technol. Biotechnol. 90 (8): 1364–1379. https://doi.org/10.1002/jctb.4551.
Valentino, F., M. Beccari, S. Fraraccio, G. Zanaroli, and M. Majone. 2014. “Feed frequency in a Sequencing Batch Reactor strongly affects the production of polyhydroxyalkanoates (PHAs) from volatile fatty acids.” New Biotechnol. 31 (4): 264–275. https://doi.org/10.1016/j.nbt.2013.10.006.
Valentino, F., M. Gottardo, F. Micolucci, P. Pavan, D. Bolzonella, S. Rossetti, and M. Majone. 2018. “Organic fraction of municipal solid waste recovery by conversion into added-value polyhydroxyalkanoates and biogas.” ACS Sustainable Chem. Eng. 6 (12): 16375–16385. https://doi.org/10.1021/acssuschemeng.8b03454.
Valentino, F., F. Morgan-Sagastume, S. Campanari, M. Villano, A. Werker, and M. Majone. 2017. “Carbon recovery from wastewater through bioconversion into biodegradable polymers.” New Biotechnol. 37: 9–23. https://doi.org/10.1016/j.nbt.2016.05.007.
Venkata Mohan, S. 2009. “Harnessing of biohydrogen from wastewater treatment using mixed fermentative consortia: Process evaluation towards optimization.” Int. J. Hydrogen Energy 34 (17): 7460–7474. https://doi.org/10.1016/j.ijhydene.2009.05.062.
Wu, S. L., G. Luo, J. Sun, W. Wei, L. Song, and B. J. Ni. 2021. “Medium chain fatty acids production from anaerobic fermentation of waste activated sludge.” J. Cleaner Prod. 279: 123482. https://doi.org/10.1016/j.jclepro.2020.123482.
Xing, Y., S. Fan, J. Zhang, Y. Fan, and H. Hou. 2011. “Enhanced bio-hydrogen production from corn stalk by anaerobic fermentation using response surface methodology.” Int. J. Hydrog. Energy 36 (20): 12770–12779. https://doi.org/10.1016/j.ijhydene.2011.07.065.
Zhang, J., H. Liu, Y. Zhang, P. Wu, J. Li, P. Ding, Q. Jiang, and M. H. Cui. 2022. “Heterotrophic precultivation is a better strategy than polarity reversal for the startup of acetate microbial electrosynthesis reactor.” Biochem. Eng. J. 179 (December 2021): 108319. https://doi.org/10.1016/j.bej.2021.108319.
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Published In
Journal of Hazardous, Toxic, and Radioactive Waste
Volume 27 • Issue 4 • October 2023
Copyright
© 2023 American Society of Civil Engineers.
History
Received: Dec 30, 2022
Accepted: Apr 6, 2023
Published online: May 31, 2023
Published in print: Oct 1, 2023
Discussion open until: Oct 31, 2023
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