Technical Papers
Nov 9, 2016

Effect of Soil Type on the Enzymatic Calcium Carbonate Precipitation Process Used for Soil Improvement

Publication: Journal of Materials in Civil Engineering
Volume 29, Issue 4

Abstract

This work analyzes the effect of soil type on the process of enzymatic calcium carbonate (CaCO3) precipitation. This methodology is tested for the stabilization of five soil types (poorly graded sand, two silty sands, a silty soil, and an organic soil) in order to examine the impact on strength and stiffness, based on the results of unconfined compression strength (UCS) and scanning electron microscopy (SEM) tests. The results of the UCS tests show that, in the case of the sandy and silty soils, the process of enzymatic calcium carbonate (CaCO3) precipitation potentiates the strengthening of the soils while, in the organic soil, a detrimental biostabilization impact is obtained. The SEM tests show the existence of vestiges of calcium in all the soils, which is a sign that the precipitation of CaCO3 does in fact occur but with a different impact on the strengthening for each soil type. The results suggest that the low pH value of the organic soil, combined with its organic coating of the soil particles, are the key factors that explain the inefficiency of the biostabilization process concerning this type of soil.

Get full access to this article

View all available purchase options and get full access to this article.

Acknowledgments

This work was supported by FCT (Portuguese Foundation for Science and Technology), within ISISE, project UID/ECI/04029/2013.

References

Al Qabany, A., and Soga, K. (2013). “Effect of chemical treatment used in MICP on engineering properties of cemented soils.” Géotechnique, 63(4), 331–339.
ASTM. (2000). “Standard practice for classification of soils for engineering purposes (unified soil classification system).” ASTM D2487, West Conshohocken, PA.
ASTM. (2003). “Standard test methods for laboratory compaction characteristics of soil using standard effort [12,400 ft-lbf/ft3 (600 kN m/m3)].” ASTM D698, West Conshohocken, PA.
ASTM. (2005). “Standard test method for unconfined compressive strength of cohesive soil.” ASTM D2166, West Conshohocken, PA.
Blakeley, R. L., and Zerner, B. (1984). “Jack bean urease: The first nickel enzyme.” J. Mol. Catal., 23(2–3), 263–292.
Burbank, M., Weaver, T., Lewis, R., Williams, T., Williams, B., and Crawford, R. (2013). “Geotechnical tests of sands following bioinduced calcite precipitation catalyzed by indigenous bacteria.” J. Geotech. Geoenviron. Eng., 928–936.
Carmona, J. P. S. F. (2016). “Use of biotecnology for soil stabilization: Enzymatic CaCO3 precipitation.” M.Sc. dissertation, Univ. of Coimbra, Coimbra, Portugal (in Portuguese).
CEN (European Committee for Standardization). (2000). “Concrete—Part 1: Specification, performance, production and conformity.” EN 206-1, Brussels, Belgium.
Cheng, L., Cord-Ruwisch, R., and Shahin, M. A. (2013). “Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation.” Can. Geotech. J., 50(1), 81–90.
Chou, C. W., Seagren, E. A., Aydilek, A. H., and Lai, M. (2011). “Biocalcification of sand through ureolysis.” J. Geotech. Geoenviron. Eng., 1179–1189.
Chu, J., Stabnikov, V., and Ivanov, V. (2012). “Microbially induced calcium carbonate precipitation on surface or in the bulk of soil.” Geomicrobiol. J., 29(6), 544–549.
Dejong, J. T., Mortensen, B. M., Martinez, B. C., and Nelson, D. C. (2010). “Bio-mediated soil improvement.” Ecol. Eng., 36(2), 197–210.
Freitas, L. D. (2016). “Use of biotecnology for soil stabilization: Enzymatic CaCO3 precipitation: Effect of soil type.” M.Sc. dissertation, Univ. of Coimbra, Coimbra, Portugal (in Portuguese).
Hammes, F., and Verstraete, W. (2002). “Key roles of pH and calcium metabolism in microbial carbonate precipitation.” Rev. Environ. Sci Biotechnol., 1(1), 3–7.
Ismail, M. A., Joer, H. A., Randolph, M. F., and Meritt, A. (2002). “Cementation of porous materials using calcite.” Géotechnique, 52(5), 313–324.
Mortensen, B. M., Haber, M. J., DeJong, J. T., Caslake, L. F., and Nelson, D. C. (2011). “Effects of environmental factors on microbial induced calcium carbonate precipitation.” J. Appl. Microbiol., 111(2), 338–349.
Muynck, W. D., Belie, N. D., and Verstraete, W. (2010). “Microbial carbonate precipitation in construction materials: A review.” Ecol. Eng., 36(2), 118–136.
Nemati, M., Voordouw, G. (2003). “Modification of porous media permeability, using calcium carbonate produced enzymatically in situ.” Enzyme Microbial Technol., 33(5), 635–642.
Neupane, D., Yasuhara, H., and Kinoshita, N. (2015a). “Evaluation of enzyme mediated calcite grouting as a possible improvement technique.” Computer methods and recent advances in geomechanics, Taylor & Francis, London, 1160–1172.
Neupane, D., Yasuhara, H., Kinoshita, N., and Unno, T. (2013). “Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique.” J. Geotech. Geoenviron. Eng., 2201–2211.
Neupane, D., Yasuhara, H., Kinoshita, N., and Unno, T. (2015b). “Distribution of mineralized carbonate and its quantification method in enzyme mediated calcite precipitation technique.” Soils Found., 55(2), 447–457.
Pansu, M., and Gautheyrou, J. (2006). Handbook of soil analysis—Mineralogical, organic and inorganic methods, Springer, Berlin.
Rebata-Landa, V. (2007). “Microbial activity in sediments: Effects on soil behavior.” Ph.D. dissertation, School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta.
Rebata-Landa, V., and Santamarina, J. C. (2006). “Mechanical limits to microbial activity in deep sediments.” Geochem. Geophys. Geosyst., 7(11), 1–12.
Stocks-Fisher, S., Galinat, J. K., and Bang, S. S. (1999). “Microbiological precipitation of CaCO3.” Soil Biol. Biochem., 31(11), 1563–1571.
Van Paassen, L. A., Ghose, R., van der Linden, T. J. M., van der Star, W. R. L., and van Loosdrecht, M. C. M. (2010a). “Potential soil reinforcement by biological denitrification.” Ecol. Eng., 36(2), 168–175.
Van Paassen, L. A., Ghose, R., van der Linden, T. J. M., van der Star, W. R. L., and van Loosdrecht, M. C. M. (2010b). “Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment.” J. Geotech. Geoenviron. Eng., 1721–1728.
Venda Oliveira, P. J., Costa, M. S., Costa, J. N. P., and Nobre, M. F. (2015). “Comparison of the ability of two bacteria to improve the behaviour of a sandy soil.” J. Mater. Civ. Eng., .
Whiffin, V. S., van Paassen, L. A., and Harkes, M. P. (2007). “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. J., 24(5), 417–423.
Yasuhara, H., Neupane, D., Hayashi, K., and Okamura, M. (2012). “Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation.” Soils Found., 52(3), 539–549.

Information & Authors

Information

Published In

Go to Journal of Materials in Civil Engineering
Journal of Materials in Civil Engineering
Volume 29Issue 4April 2017

History

Received: Apr 18, 2016
Accepted: Aug 31, 2016
Published online: Nov 9, 2016
Published in print: Apr 1, 2017
Discussion open until: Apr 9, 2017

Permissions

Request permissions for this article.

Authors

Affiliations

Paulo J. Venda Oliveira [email protected]
Assistant Professor, Dept. of Civil Engineering, Univ. of Coimbra, R. Luís Reis Santos, 3030-788 Coimbra, Portugal (corresponding author). E-mail: [email protected]
Luís D. Freitas [email protected]
Master Student, Dept. of Civil Engineering, Univ. of Coimbra, R. Luís Reis Santos, 3030-788 Coimbra, Portugal. E-mail: [email protected]
João P. S. F. Carmona [email protected]
Civil Engineer, Dept. of Civil Engineering, Univ. of Coimbra, R. Luís Reis Santos, 3030-788 Coimbra, Portugal. E-mail: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share