Impact of Hydraulic Loading Rate on the Removal Performance and Filter-Bed Clogging of Horizontal-Subsurface-Flow Macrophyte-Assisted Vermifilter Treating Dairy Wastewater
Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 26, Issue 3
Abstract
Vermifilter is a cost-effective and environmentally friendly technology for domestic and industrial wastewater treatment. However, filter bed clogging is an operational challenge of vermifilter. The present study was conducted to investigate the impact of different hydraulic loading rates (HLRs) on treatment performance and filter bed clogging of horizontal subsurface flow macrophyte-assisted vermifilters (HSSF-MAVF). Three laboratory-scale horizontal subsurface flow vermifilter reactors planted with Canna indica were used in this study. Each reactor was fed synthetic dairy wastewater with HLRs of 0.66, 1.5, and 2.34 m/day, respectively. Based upon the results of this study, the treatment performance and filter bed clogging of the system were observed to be closely dependent on the applied HLRs. The highest treatment performance (89 ± 2%, 94 ± 2%, 95 ± 4%, and 76 ± 5%, respectively, for COD, TSS, ammonium-N, and phosphate-P) with minimum filter bed clogging (10 ± 2% hydraulic conductivity reduction and 0.19 ± 0.05-cm head loss) was observed in the reactor fed with the lowest HLR of 0.66 m/day. Similarly, the lowest treatment performance (67 ± 3%, 61 ± 4%, 87 ± 2%, and 63 ± 4%, respectively, for COD, TSS, ammonium-N, and phosphate-P) with severe clogging of the filter bed (33 ± 2% hydraulic conductivity reduction and 2.1 ± 0.5-cm head loss) was observed in the reactor fed with the highest HLR of 2.34 m/day. The clog matter (CM) from each filter bed is composed of both organic and inorganic materials, and the quantity of CM varied from 6.46 ± 0.28 to 24.44 ± 1-mg/mL, with protein being the major organic clog matter component.
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Acknowledgments
The authors are thankful to the Department of Civil Engineering, School of Infrastructure, Indian Institute of Technology, Bhubaneswar, India, for providing facilities for carrying out research work in the related area.
References
APHA (American Public Health Association). 2005. Standard methods for the examination of water and wastewater. 21st ed. Washington, DC: APHA.
Avsar, Y., H. Tarabeah, S. Kimchie, and I. Ozturk. 2007. “Rehabilitation by constructed wetlands of available wastewater treatment plant in Sakhnin.” Ecol. Eng. 29 (1): 27–32. https://doi.org/10.1016/j.ecoleng.2006.07.008.
Bajsa, O., J. Nair, K. Mathew, and G. E. Ho. 2003. “Vermiculture as a tool for domestic wastewater management.” Water Sci. Technol. 48 (11–12): 125–132. https://doi.org/10.2166/wst.2004.0821.
Berendsen, R. L., C. M. Pieterse, and P. A. Bakker. 2012. “The rhizosphere microbiome and plant health.” Trends Plant Sci. 17 (8): 478–486. https://doi.org/10.1016/j.tplants.2012.04.001.
Billore, S. K., N. Singh, H. K. Ram, J. K. Sharma, V. P. Singh, R. M. Nelson, and P. Dass. 2001. “Treatment of a molasses based distillery effluent in a constructed wetland in central India.” Water Sci. Technol. 44 (11–12): 441–448. https://doi.org/10.2166/wst.2001.0864.
Bo, F., R. Palmgren, K. Keiding, and P. H. Nielsen. 1996. “Extraction of extracellular polymers from activated sludge using a cation exchange resin.” Water Res. 30 (8): 1749–1758. https://doi.org/10.1016/0043-1354(95)00323-1.
Bottinelli, N., T. Henry-des-Tureaux, V. Hallaire, J. Mathieu, Y. Benard, T. D. Tran, and P. Jouquet. 2010. “Earthworms accelerate soil porosity turnover under watering conditions.” Geoderma 156 (1–2): 43–47. https://doi.org/10.1016/j.geoderma.2010.01.006.
Bradford, M. M. 1996. “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.” Anal. Biochem. 72 (1–2): 248–254. https://doi.org/10.1016/0003-2697(76)90527-3.
Button, M., J. Nivala, K. P. Weber, T. Aubron, and R. A. Müller. 2015. “Microbial community metabolic function in subsurface flow constructed wetlands of different designs.” Ecol. Eng. 80: 162–171. https://doi.org/10.1016/j.ecoleng.2014.09.073.
Calheiros, C. S., A. O. Rangel, and P. M. Castro. 2007. “Constructed wetland systems vegetated with different plants applied to the treatment of tannery wastewater.” Water Res. 41 (8): 1790–1798. https://doi.org/10.1016/j.watres.2007.01.012.
Chokshi, K., I. Pancha, A. Ghosh, and S. Mishra. 2016. “Microalgal biomass generation by phycoremediation of dairy industry wastewater: An integrated approach towards sustainable biofuel production.” Bioresour. Technol. 221: 455–460. https://doi.org/10.1016/j.biortech.2016.09.070.
Chowdhury, S. D., and P. Bhunia. 2021. “Simultaneous carbon and nitrogen removal from domestic wastewater using high rate vermifilter.” Indian J. Microbiol. 61 (2): 218–228. https://doi.org/10.1007/s12088-021-00936-4.
Dan, T. H., L. N. Quang, N. H. Chiem, and H. Brix. 2011. “Treatment of high-strength wastewater in tropical constructed wetlands planted with Sesbania sesban: Horizontal subsurface flow versus vertical down flow.” Ecol. Eng. 37 (5): 711–720. https://doi.org/10.1016/j.ecoleng.2010.07.030.
De Matos, M. P., M. Von Sperling, and A. T. de Matos. 2018. “Clogging in horizontal subsurface flow constructed wetlands: Influencing factors, research methods and remediation techniques.” Rev. Environ. Sci. Biotechnol. 17 (1): 87–107. https://doi.org/10.1007/s11157-018-9458-1.
Demirel, B., O. Yenigun, and T. O. Turgut. 2005. “Anaerobic treatment of dairy wastewaters: A review.” Process Biochem. 40 (8): 2583–2595. https://doi.org/10.1016/j.procbio.2004.12.015.
Frolund, B., T. Griebe, and P. H. Nielsen. 1995. “Enzymatic activity in the activated sludge floc matrix.” Appl. Microbiol. Biotechnol. 43 (4): 755–761. https://doi.org/10.1007/s002530050481.
Ghosh, D., and B. Gopal. 2010. “Effect of hydraulic retention time on the treatment of secondary effluent in a subsurface flow constructed wetland.” Ecol. Eng. 36 (8): 1044–1051. https://doi.org/10.1016/j.ecoleng.2010.04.017.
Gunadi, B., C. Blount, and C. A. Edwards. 2002. “The growth and fecundity of Eisenia fetida (Savigny) in cattle solids pre-composted for different periods.” Pedobiologia 46 (1): 15–23. https://doi.org/10.1078/0031-4056-00109.
Hait, S., and V. Tare. 2011. “Vermistabilization of primary sewage sludge.” Bioresour. Technol. 102 (3): 2812–2820. https://doi.org/10.1016/j.biortech.2010.10.031.
Hua, G., Y. Cheng, J. Kong, M. Li, and Z. Zhao. 2018. “High-throughput sequencing analysis of bacterial community spatiotemporal distribution in response to clogging in vertical flow constructed wetlands.” Bioresour. Technol. 248: 104–112. https://doi.org/10.1016/j.biortech.2017.07.061.
Hua, G. F., L. Li, Y. Q. Zhao, W. Zhu, and J. Q. Shen. 2013. “An integrated model of substrate clogging in vertical flow constructed wetlands.” J. Environ. Manage. 119: 67–75. https://doi.org/10.1016/j.jenvman.2013.01.023.
IWA. 2000. Constructed wetlands for pollution control. IWA special group on use of macrophytes in water pollution control science and technology. Rep. 8. London: IWA Publishing.
Jiang, L., Y. Liu, X. Hu, G. Zeng, H. Wang, L. Zhou, X. Tan, B. Huang, S. Liu, and S. Liu. 2016. “The use of microbial-earthworm eco filters for wastewater treatment with special attention to influencing factors in performance: A review.” Bioresour. Technol. 200: 999–1007. https://doi.org/10.1016/j.biortech.2015.11.011.
Kadam, A., G. Oza, P. Nemade, S. Dutta, and H. Shankar. 2008. “Municipal wastewater treatment using novel constructed soil filter system.” Chemosphere 71 (5): 975–981. https://doi.org/10.1016/j.chemosphere.2007.11.048.
Knowles, P., G. Dotro, J. Nivala, and J. Garcia. 2011. “Clogging in subsurface-flow treatment wetlands: Occurrence and contributing factors.” Ecol. Eng. 37 (2): 99–112. https://doi.org/10.1016/j.ecoleng.2010.08.005.
Kumar, T., A. Rajpal, R. Bhargva, and K. S. H. Prasad. 2014. “Performance evaluation of vermifilter at different hydraulic loading rates using river bed material.” Ecol. Eng 62: 77–82. https://doi.org/10.1016/j.ecoleng.2013.10.028.
Licciardello, F., R. Aiello, V. Alagna, M. Iovino, D. Ventura, and G. L. Cirelli. 2019. “Assessment of clogging in constructed wetlands by saturated hydraulic conductivity measurements.” Water Sci. Technol. 79 (2): 314–322. https://doi.org/10.2166/wst.2019.045.
Liu, H., and H. H. P. Fang. 2002. “Extraction of extracellular polymeric substances (EPS) of sludge.” J. Biotechnol. 95 (3): 249–256. https://doi.org/10.1016/S0168-1656(02)00025-1.
Metcalf, E., 1991. Wastewater engineering. 3rd ed. New York: McGraw-Hill.
Nemade, P. D., A. M. Kadam, and H. S. Shankar. 2009. “Wastewater renovation using constructed soil filter (CSF): A novel approach.” J. Hazard. Mater. 170 (2–3): 657–665. https://doi.org/10.1016/j.jhazmat.2009.05.015.
Pedescoll, A., R. Samso, E. Romero, J. Puigagut, and J. Garcia. 2011. “Reliability, repeatability and accuracy of the falling head method for hydraulic conductivity measurements under laboratory conditions.” Ecol. Eng. 37 (5): 754–757. https://doi.org/10.1016/j.ecoleng.2010.06.032.
Petitjean, A., N. Forquet, and C. Boutin. 2016. “Oxygen profile and clogging in vertical flow sand filters for on-site wastewater treatment.” J. Environ. Manage. 170: 15–20. https://doi.org/10.1016/j.jenvman.2015.12.033.
Reinecke, A. J., and S. A. Viljoen. 1990. “The influence of feeding patterns on growth and reproduction of the vermicomposting earthworm Eisenia fetida (Oligochaeta).” Biol. Fertil. Soils 10: 184–187.
Reinecke, A. J., and J. M. Venter. 1987. “Moisture preferences, growth and reproduction of the compost worm Eisenia fetida (Oligochaeta).” Biol. Fertil. Soils 3: 135–141.
Samal, K., R. R. Dash, and P. Bhunia. 2017a. “Treatment of wastewater by vermifiltration integrated with macrophyte filter: A review.” J. Environ. Chem. Eng. 5 (3): 2274–2289. https://doi.org/10.1016/j.jece.2017.04.026.
Samal, K., R. R. Dash, and P. Bhunia. 2017b. “Performance assessment of a Canna indica assisted vermifilter for synthetic dairy wastewater treatment.” Process. Saf. Environ. Prot. 111: 363–374. https://doi.org/10.1016/j.psep.2017.07.027.
Samal, K., R. R. Dash, and P. Bhunia. 2018a. “A comparative study of macrophytes influence on the performance of hybrid vermifilter for dairy wastewater treatment.” J. Environ. Chem. Eng. 6 (4): 4714–4726. https://doi.org/10.1016/j.jece.2018.07.018.
Samal, K., R. R. Dash, and P. Bhunia. 2018b. “Effect of hydraulic loading rate and pollutants degradation kinetics in two-stage hybrid macrophyte assisted vermifiltration system.” Biochem. Eng. J. 132: 47–59. https://doi.org/10.1016/j.bej.2018.01.002.
Samal, K., R. Singh, R. R. Dash, and P. Bhunia. 2020. “Investigation on the effect of planting Canna indica in two-stage vermifilter for synthetic dairy wastewater treatment.” In Recent developments in waste management, edited by A. Kalamdhad, 289–297. Singapore: Springer. https://doi.org/10.1007/978-981-15-0990-2_22.
Shepherd, H. L., M. E. Grismer, and G. Tchobanoglous. 2001. “Treatment of high-strength winery wastewater using a subsurface-flow constructed wetland.” Water Environ. Res. 73 (4): 394–403. https://doi.org/10.2175/106143001X139434.
Singh, R., M. D. Alessio, Y. Meneses, S. L. Bartelt-Hunt, B. Woodbury, and C. Ray. 2021. “Development and performance assessment of an integrated vermifiltration based treatment system for the treatment of feedlot runoff.” J. Clean. Prod. 278: 123355. https://doi.org/10.1016/j.jclepro.2020.123355.
Singh, R., P. Bhunia, and R. R. Dash. 2018. “Understanding intricacies of clogging and its alleviation by introducing earthworms in soil biofilters.” Sci. Total Environ. 633: 145–156. https://doi.org/10.1016/j.scitotenv.2018.03.156.
Singh, R., P. Bhunia, and R. R. Dash. 2019a. “Optimization of organics removal and understanding the impact of HRT on vermifiltration of brewery wastewater.” Sci. Total Environ. 651: 283–1293.
Singh, R., P. Bhunia, and R. R. Dash. 2019c. ““Impact of organic loading rate and earthworms on dissolved oxygen and vermifiltration.” J. Hazard. Toxic Radioact. Waste 23 (2): 04019001. https://doi.org/10.1061/(ASCE)HZ.2153-5515.0000435.
Singh, R., P. Bhunia, and R. R. Dash. 2019b. “Optimization of bio-clogging in the vermifilters: A statistical approach.” J. Environ. Manage. 233: 576–585. https://doi.org/10.1016/j.jenvman.2018.12.065.
Singh, R., P. Bhunia, and R. R. Dash. 2017. “A mechanistic review on vermifiltration of wastewater: Design, operation and performance.” J. Environ. Manage. 197: 656–672. https://doi.org/10.1016/j.jenvman.2017.04.042.
Sinha, R. K., G. Bharambe, and U. Chaudhari. 2008. “Sewage treatment by vermifiltration with synchronous treatment of sludge by earthworms: A low cost sustainable technology over conventional systems with potential for decentralization.” Environmentalist 28 (4): 409–420. https://doi.org/10.1007/s10669-008-9162-8.
Song, Z., C. J. Williams, and R. G. J. Edyvean. 2000. “Sedimentation of tannery wastewater.” Water Res. 34 (7): 2171–2176. https://doi.org/10.1016/S0043-1354(99)00358-9.
Suhaib, K. H., and P. Bhunia. 2022. “Dynamics of clogging in subsurface flow constructed wetlands.” J. Hazard. Toxic Radioact. Waste 26 (1): 03121004. https://doi.org/10.1061/(ASCE)HZ.2153-5515.0000646.
Tang, P., B. Yu, Y. Zhou, Y. Zhang, and J. Li. 2017. “Clogging development and hydraulic performance of the horizontal subsurface flow storm water constructed wetlands: A laboratory study.” Environ. Sci. Pollut. Res. 24 (10): 9210–9219. https://doi.org/10.1007/s11356-017-8458-y.
Vymazal, J. 2018. “Does clogging affect long-term removal of organics and suspended solids in gravel-based horizontal subsurface flow constructed wetlands?” Chem. Eng. J. 331: 663–674. https://doi.org/10.1016/j.cej.2017.09.048.
Wang, L., F. Guo, Z. Zheng, and J. Zhang. 2011a. “Enhancement of rural domestic sewage treatment performance, and assessment of microbial community diversity and structure using tower vermifiltration.” Bioresour. Technol. 102: 9462–9470. https://doi.org/10.1016/j.biortech.2011.07.085.
Wang, L., Z. Guo, Y. Che, F. Yang, J. Chao, Y. Gao, and Y. Zhang. 2014. “The effect of vermifiltration height and wet:dry time ratio on nutrient removal performance and biological features, and their influence on nutrient removal efficiencies.” Ecol. Eng. 71: 165–172. https://doi.org/10.1016/j.ecoleng.2014.07.018.
Wang, L., Z. Zheng, X. Luo, and J. Zhang. 2011b. “Performance and mechanisms of a microbial-earthworm ecofilter for removing organic matter and nitrogen from synthetic domestic wastewater.” J. Hazard. Mater. 195: 245–253. https://doi.org/10.1016/j.jhazmat.2011.08.035.
Wu, H., J. Zhang, H. H. Ngo, W. Guo, Z. Hu, S. Liang, J. Fan, and H. Liu. 2015. “A review on the sustainability of constructed wetlands for wastewater treatment: Design and operation.” Bioresour. Technol. 175: 594–601. https://doi.org/10.1016/j.biortech.2014.10.068.
Wu, S., S. He, W. Zhou, J. Gu, J. Huang, L. Gao, and X. Zhang. 2017. “Decomposition characteristics of three different kinds of aquatic macrophytes and their potential application as carbon resource in constructed wetland.” Environ. Pollut. 231: 1122–1133. https://doi.org/10.1016/j.envpol.2017.07.049.
Xing, M., X. Li, and J. Yang. 2010. “Treatment performance of small-scale vermifilter for domestic wastewater and its relationship to earthworm growth, reproduction and enzymatic activity.” Afr. J. Biotechnol. 9 (44): 7513–7520. https://doi.org/10.5897/AJB10.811.
Xu, J., C. Li, F. Yang, Z. Dong, J. Zhang, Y. Zhao, P. Qi, and Z. Hu. 2011. “Typha angustifolia stress tolerance to wastewater with different levels of chemical oxygen demand.” Desalination 280 (1–3): 58–62. https://doi.org/10.1016/j.desal.2011.06.050.
Xu, J., J. Zhang, H. Xie, C. Li, N. Bao, C. Zhang, and Q. Shi. 2010. “Physiological responses of Phragmites australis to wastewater with different chemical oxygen demands.” Ecol. Eng. 36 (10): 1341–1347. https://doi.org/10.1016/j.ecoleng.2010.06.010.
Xu, Q., L. Cui, and L. Zhang. 2014. “Load working together on soil clogging on vertical flow constructed wetland.” Environ. Sci. Technol. 37: 1–5. https://doi.org/10.1021/es025799o.
Ye, C. 2002. Research on components and spatial distribution of clog matter in vertical-flow constructed wetland. Nanjing, China: Hohai Univ.
Ye, J., H. Li, C. Zhang, C. Ye, and W. Han. 2014. “Classification and extraction methods of the clog components of constructed wetland.” Ecol. Eng. 70: 327–331. https://doi.org/10.1016/j.ecoleng.2014.06.028.
Ye, J., Z. Xu, H. Chen, L. Wang, and G. Benoit. 2018. “Reduction of clog matter in constructed wetlands by metabolism of Eisenia fetida: Process and modeling.” Environ. Pollut. 238: 803–811. https://doi.org/10.1016/j.envpol.2018.03.062.
Zhang, M., D. Xu, G. Bai, T. Cao, W. Liu, Z. Hu, D. Chen, D. Qiu, and Z. Wu. 2021. “Changes of microbial community structure during the initial stage of biological clogging in horizontal subsurface flow constructed wetlands.” Bioresour. Technol. 337: 125405. https://doi.org/10.1016/j.biortech.2021.125405.
Zhao, Y. Q., G. Sun, and S. J. Allen. 2004. “Anti-sized reed bed system for animal wastewater treatment: A comparative study.” Water Res. 38 (12): 2907–2917. https://doi.org/10.1016/j.watres.2004.03.038.
Zhao, Y., Y. Zhang, Z. Ge, C. Hu, and H. Zhang. 2014. “Effects of influent C/N ratios on wastewater nutrient removal and simultaneous greenhouse gas emission from the combinations of vertical subsurface flow constructed wetlands and earthworm eco-filters for treating synthetic wastewater.” Environ. Sci. Process. Impact. 16 (3): 567–575. https://doi.org/10.1039/c3em00655g.
Zhu, Y., P. Ye, S. Xu, Y. Zhou, Y. Zhang, Y. Zhang, and T. Zhang. 2020. “The influence mechanism of bioclogging on pollution removal efficiency of vertical flow constructed wetland.” Water Sci. Technol. 81 (9): 1870–1881. https://doi.org/10.2166/wst.2020.246.
Zhou, X., Z. Chen, Z. Li, and H. Wu. 2020. “Impacts of aeration and biochar addition on extracellular polymeric substances and microbial communities in constructed wetlands for low C/N wastewater treatment: Implications for clogging.” Chem. Eng. J. 396: 125349. https://doi.org/10.1016/j.cej.2020.125349.
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Journal of Hazardous, Toxic, and Radioactive Waste
Volume 26 • Issue 3 • July 2022
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© 2022 American Society of Civil Engineers.
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Received: Oct 16, 2021
Accepted: Jan 6, 2022
Published online: Feb 23, 2022
Published in print: Jul 1, 2022
Discussion open until: Jul 23, 2022
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