Technical Papers
Oct 8, 2012

Observation of the pH-Induced Hormesis for the LDH Cytotoxicity Test of MSWI Baghouse Ash Extract

Publication: Journal of Hazardous, Toxic, and Radioactive Waste
Volume 17, Issue 2

Abstract

The environmental impact of a toxicant can be assessed using many biological toxicity indexes. Many reports describe the ecotoxicity by E. Coli and lactate dehydrogenase (LDH) cytotoxicity of ash wastes obtained from municipal solid waste incineration (MSWI) ash wastes. The experimental cytotoxicity test of MSWI ash waste extract sometimes showed a negative or greater than 100% (hormesis phenomenon) value using Vero cells. Parallel experiments using E. Coli and Vero cells were conducted for serious MSWI ash waste extracts with different pH values. In this paper, the pH-induced hormesis phenomenon was confirmed to be observed on the LDH cytotoxicity test for MSWI ash extract at acidic condition (pH<5). The application of this study is that E. Coli can be used as an online biosensor for MSWI ash wastewater generated from an ash recycling system, and the hormesis effect shall be considered in the cytotoxicity explanation of such MSWI ash samples.

Get full access to this article

View all available purchase options and get full access to this article.

Acknowledgments

The authors thank the National Science Council of the Republic of China, who provided financial support of this research under contract number NSC 98-2221-E-020-004.

References

Ali, M., et al. (2004). “Fly ash leachate induced oxidative stress in freshwater fish Channa punctata (bloch).” Environ. Int., 30(7), 933–938.
Ali, M., Rahman, S., Rehman, H., Bhataia, K., Ansari, R. A., and Raisuddin, S. (2007). “Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch).” Toxicol. In Vitro, 21(1), 63–71.
Azzam, E. I. (2011). “Exposure to low level environmental agents: The induction hormesis.” Mutat. Res., 726(2), 89–90.
Bernardo, M. S., et al. (2009). “Chemical and ecotoxicological characterization of solid residues produced during the copyrolysis of plastics and pine biomass.” J. Hazard. Mater., 166(1), 309–317.
Berney, M., Weilenmann, H.-U., and Egli, T. (2007). “Adaptation to UVA radiation of E. coli growing in continuous culture.” J. Photochem. Photobiol., B, 86(2), 149–159.
Calabrase, E. J. (2004). “Hormesis: A revolution in toxicology, risk assessment and medicine.” EMBO Rep., 5(1), S37–S40.
Calabrase, E. J., and Blain, R. (2011). “The hormesis database: The occurrence of hormetic dose response in the ecological literatures.” Regul. Toxicol. Pharmacol., 61(1), 73–81.
Chen, B.-Y., and Lin, K.-L. (2006). “Biotoxicity assessment on reusability of municipal solid waste incinerator (MSWI) ash.” J. Hazard. Mater., 136(3), 741–746.
Chen, B.-Y., and Lin, K.-L. (2007). “Dose–mortality assessment on municipal solid waste incinerator (MSWI) ash.” J. Hazard. Mater., 139(1), 19–24.
Chou, J. D., Wey, M.-Y., Liang, H.-H., and Chang, S. H. (2009). “Biotoxicity evaluation of fly ash and bottom ash from different municipal solid waste incinerators.” J. Hazard. Mater., 168(1), 197–202.
Diabate, S., Mulhopt, S., Paul, H.-R., Wottrich, R., and Krug, H. F. (2002). “In vitro effects of incineration fly ash on pulmonary macrophages and epithelial cells.” Int. J. Hyg. Environ. Health, 204(5–6), 323–326.
Foppen, I. W. A., and Schijven, J. F. (2006). “Evaluation of data from the literature on the transport and survival of Escherichia coli and thermotolerant coliforms in aquifers under saturated conditions.” Water Res., 40(3), 401–426.
Fujikawa, H., Kai, A., and Morozumi, S. (2004). “A new logistic model for Escherichia coli growth at constant and dynamic temperatures.” Food Microbiol., 21(5), 501–504.
Gilmour, M. I., Connor, S. O., Dick, C. A. J., Moller, C. A., and Linak, W. P. (2004). “Differential pulmonary inflammation and in vitro cytotoxicity of size-fractionated fly ash particles from pulverized coal combustion.” J. Air Waste Manage. Assoc., 54(3), 286–295.
Huang, W.-J., and Shue, M.-F. (2007). “Cytotoxicity of the exhausted gas from a thermal reactor of MSWI baghouse ash.” Chemosphere, 69(6), 967–971.
Huang, W.-J., Tang, H.-C., Lin, K.-L., and Liao, M.-H. (2010). “An emerging pollutant contributing to the cytotoxicity of MSWI ash wastes: Strontium.” J. Hazard. Mater., 173(1–3), 597–604.
Huang, W.-J., Tsai, J.-L., and Liao, M.-H. (2008). “Cytotoxicity studies of ash wastes from MSWI by the testing of animal kidney cells.” Chemosphere, 71(10), 1860–1865.
Hung, C.-M., et al. (2012). “Preparation, performance, and cytotoxicity evaluation of nanoarchitectures CuO/CeO2 composite catalysts for the catalytic decomposition of ammonia.” J. Hazard. Toxic Radioact. Waste, 16(1), 58–62.
ISO. (1999). "Biological evaluation of medical devices—Part 5: Test for in vitro cytotoxicity." 10993-5, Geneva.
Jiang, N., et al. (2000). “Residual oil fly ash induces cytotoxicity and mucin secretion by guinea pig tracheal epithelial cells via an oxidant-mediated mechanism.” Toxicol. Appl. Pharmacol., 163(3), 221–230.
Lapa, N., Barbosa, R., Morais, J., Mendes, B., Mehu, J., and Santos Oliverira, J. F. (2002). “Ecotoxicological assessment of Leachates from MSWI bottom ashes.” Waste Manage., 22(6), 583–593.
Sil, A., et al. (2012). “Toxicity characteristics of drilling mud and its effect on aquatic fish populations.” J. Hazard. Toxic Radioact. Waste, 16(1), 51–57.
Soto, A. M., Sonnenschein, C., Chung, K. L., Fernandez, M. F., Olea, N., and Serrano, F. O. (1995). “The E-SCREEN assay as a tool to identify estrogen: An update on estrogenic environmental pollutants.” Environ. Health Perspect., 103(Suppl. 7), 113–122.
Standardization Administration of the People's Republic of China (SAC). (2003). “Biological evaluation of medical devices—Part 5: Test for in vitro cytotoxicity.” GB/T 16886.5-2003, Beijing.
Tsiridis, V., Samaras, P., Kungolos, A., and Sakellaropoulos, G. P. (2006). “Application of leaching test for toxicity evaluation of coal fly ash.” Environ. Toxicol., 21(4), 409–416.
Xu, T.-J., and Ting, Y.-P. (2009). “Fungal bioleaching of incineration fly ash: Metal extraction and modeling growth kinetics.” Enzyme Microb. Technol., 44(5), 323–328.

Information & Authors

Information

Published In

Go to Journal of Hazardous, Toxic, and Radioactive Waste
Journal of Hazardous, Toxic, and Radioactive Waste
Volume 17Issue 2April 2013
Pages: 134 - 139

History

Received: Jul 22, 2012
Accepted: Oct 5, 2012
Published online: Oct 8, 2012
Published in print: Apr 1, 2013

Permissions

Request permissions for this article.

Authors

Affiliations

Wu-Jang Huang [email protected]
Professor, Head of Solid Waste Valorization Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan (corresponding author). E-mail: [email protected]
Kai-Fu Chang [email protected]
Master Student, Solid Waste Valorization Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Tzu-Yi Chang [email protected]
Graduated Master Student, Solid Waste Valorization Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Cheng-Hung Chen [email protected]
Graduated Master Student, Solid Waste Valorization Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Mei-Huei Wu [email protected]
Graduated Master Student, Solid Waste Valorization Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Juei-Yu Chiu [email protected]
Associate Professor, Head of Applied Microbiology Laboratory, Dept. of Environmental Engineering and Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Ming-Huei Liao [email protected]
Professor, Head of Microbiology Laboratory, Dept. of Veterinary Medicine, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]
Yan-Der Hsuuw [email protected]
Professor, Head of Embryo Development and Embryonic Stem Cells Laboratory, Dept. of Life Science, National Ping-Tung Univ. of Science and Technology, 912 Ping-Tung, Taiwan. E-mail: [email protected]

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share