Effects of Combined Ag and ZnO Nanoparticles on Microbial Communities from Crab Orchard Creek, Illinois, USA
Publication: Journal of Environmental Engineering
Volume 146, Issue 7
Abstract
The widespread use of Ag and ZnO nanoparticles (NPs) in commercial products has raised concern of their potential adverse impact on humans and the environment. These NPs most commonly enter the environment from the effluents of wastewater treatment plants (WWTPs). To understand the impact of these NPs on microbial communities, this study focused on adding environment-relevant concentrations of Ag (), ZnO (), and Ag+ZnO () to water collected from where the Carbondale Southeast WWTP effluent mixes with Crab Orchard Creek in IL, USA. Nanoparticles-spiked samples were withdrawn immediately and after 72 h for dynamic light scattering analysis. Optical density analysis by ultraviolet–visible spectroscopy, quantitative PCR analysis, and deoxyribonucleic acid sequencing were also conducted after 72 h. The results showed that ZnO NPs, at the studied concentration, were toxic, as a decrease in microbial biomass at Day 3 compared to the Control was reached, with an increase in mass fraction of nanoscale particles over time. However, when ZnO NPs were added in combination with Ag NPs, the adverse impact of ZnO NPs was mitigated, as an increase in microbial biomass was reached compared to the Control at Day 3, with a decrease in mass fraction of nanoscale particles over time. Ag NPs alone, at the studied concentration, did not bring significant impact on the microbial biomass. The relative abundance of the bacterial population affected the most by the addition of NPs was Firmicutes, with an increase in 13.74% compared to the Control when Ag+ZnO NPs were added. This study demonstrated the response of microbial communities to increased environment-relevant concentrations of NPs in a short period of time. The result will provide information that may aid in the preparation of guidance or regulations related to discharge of mixtures of NPs to surface water.
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Data Availability Statement
All data, models, and code generated or used during the study appear in the published article.
Acknowledgments
This research was sponsored by Research Enriched Academic Challenge (REACH) program, Southern Illinois University Carbondale (SIUC). Dr. Liu also acknowledges start-up funding from SIUC. Thanks are also given to Dr. Boyd M. Goodson in SIUC for DLS analysis.
References
Abachi, S., S. Lee, and H. P. Rupasinghe. 2016. “Molecular mechanisms of inhibition of Streptococcus species by phytochemicals.” Molecules 21 (2): 215. https://doi.org/10.3390/molecules21020215.
Bhatt, I., and B. N. Tripathi. 2011. “Interaction of engineered nanoparticles with various components of the environment and possible strategies for their risk assessment.” Chemosphere 82 (3): 308–317. https://doi.org/10.1016/j.chemosphere.2010.10.011.
Bomberg, M., L. Montonen, U. Münster, and G. Jurgens. 2008. “Diversity and function of archaea in freshwater habitats.” Curr. Trends Microbiol. 4: 61–69.
Clavier, A., A. Praetorius, and S. Stoll. 2019. “Determination of nanoparticle heteroaggregation attachment efficiencies and rates in presence of natural organic matter monomers. Monte Carlo modelling.” Sci. Total Environ. 650 (Feb): 530–540. https://doi.org/10.1016/j.scitotenv.2018.09.017.
Dinesh, R., M. Anandaraj, V. Srinivasan, and S. Hamza. 2012. “Engineered nanoparticles in the soil and their potential implications to microbial activity.” Supplement, Geoderma 173 (SC): 19–27. https://doi.org/10.1016/j.geoderma.2011.12.018.
Doolette, C. L., M. J. McLaughlin, J. K. Kirby, D. J. Batstone, H. H. Harris, H. Ge, and G. Cornelis. 2013. “Transformation of PVP coated silver nanoparticles in a simulated wastewater treatment process and the effect on microbial communities.” Chem. Cent. J. 7 (1): 46.
Doxey, A. C., D. A. Kurtz, M. D. J. Lynch, L. A. Sauder, and J. D. Neufeld. 2015. “Aquatic metagenomes implicate Thaumarchaeota in global cobalamin production.” ISME J. 9 (2): 461–471. https://doi.org/10.1038/ismej.2014.142.
Echavarri-Bravo, V., L. Paterson, T. J. Aspray, J. S. Porter, M. K. Winson, B. Thornton, and M. G. J. Hartl. 2015. “Shifts in the metabolic function of a benthic estuarine microbial community following a single pulse exposure to silver nanoparticles.” Supplement, Environ. Pollut. 201 (SC): 91–99. https://doi.org/10.1016/j.envpol.2015.02.033.
Fabrega, J., S. N. Luoma, C. R. Tyler, T. S. Galloway, and J. R. Lead. 2011. “Silver nanoparticles: Behaviour and effects in the aquatic environment.” Environ. Int. 37 (2): 517–531. https://doi.org/10.1016/j.envint.2010.10.012.
Farkas, J., H. Peter, T. M. Ciesielski, K. V. Thomas, R. Sommaruga, W. Salvenmoser, G. A. Weyhenmeyer, L. J. Tranvik, and B. M. Jenssen. 2015. “Impact of TiO2 nanoparticles on freshwater bacteria from three Swedish lakes.” Sci. Total Environ. 535 (Dec): 85–93. https://doi.org/10.1016/j.scitotenv.2015.03.043.
Gottschalk, F., C. Lassen, J. Kjoelholt, F. Christensen, and B. Nowack. 2015. “Modeling flows and concentrations of nine engineered nanomaterials in the Danish environment.” Int. J. Environ. Res. Publ. Health 12 (5): 5581–5602. https://doi.org/10.3390/ijerph120505581.
Gottschalk, F., T. Sonderer, R. W. Scholz, and B. Nowack. 2009. “Modeled environmental concentrations of engineered nanomaterials (TiO2, ZnO, Ag, CNT, Fullerenes) for different regions.” Environ. Sci. Technol. 43 (24): 9216–9222. https://doi.org/10.1021/es9015553.
Heithmar, E. M. 2011. Screening methods for metal-containing nanoparticles in water. Washington, DC: USEPA.
Jones, N., B. Ray, K. T. Ranjit, and A. C. Manna. 2008. “Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms.” FEMS Microbiol. Let. 279 (1): 71–76. https://doi.org/10.1111/j.1574-6968.2007.01012.x.
Kinney, C. A., E. T. Furlong, S. D. Zaugg, M. R. Burkhardt, S. L. Werner, J. D. Cahill, and G. R. Jorgensen. 2006. “Survey of organic wastewater contaminants in biosolids destined for land application.” Environ. Sci. Technol. 40 (23): 7207–7215. https://doi.org/10.1021/es0603406.
Liu, J., J. Chao, R. Liu, Z. Tan, Y. Yin, Y. Wu, and G. Jiang. 2009. “Cloud point extraction as an advantageous preconcentration approach for analysis of trace silver nanoparticles in environmental waters.” Anal. Chem. 81 (15): 6496–6502. https://doi.org/10.1021/ac900918e.
Liu, J., and C. Vipulanandan. 2013. “Effects of Au/Fe and Fe nanoparticles on Serratia bacterial growth and production of biosurfactant.” Mat. Sci. Eng. 33 (7): 3909–3915. https://doi.org/10.1016/j.msec.2013.05.026.
Liu, J., C. Vipulanandan, T. F. Cooper, and G. Vipulanandan. 2013. “Effects of Fe nanoparticles on bacterial growth and biosurfactant production.” J. Nanopart. Res. 15 (1): 1405. https://doi.org/10.1007/s11051-012-1405-4.
Liu, J., P. C. Williams, J. Geisler-Lee, B. M. Goodson, M. Fakharifar, M. Peiravi, D. Chen, D. A. Lightfoot, and M. E. Gemeinhardt. 2018a. “Impact of wastewater effluent containing aged nanoparticles and other components on biological activities of the soil microbiome, Arabidopsis plants, and earthworms.” Environ. Res. 164 (Jul): 197–203. https://doi.org/10.1016/j.envres.2018.02.006.
Liu, J., P. C. Williams, B. M. Goodson, J. Geisler-Lee, M. Fakharifar, and M. E. Gemeinhardt. 2019. “TiO2 nanoparticles in irrigation water mitigate impacts of aged Ag nanoparticles on soil microorganisms, Arabidopsis thaliana plants, and Eisenia fetida earthworms.” Environ. Res. 172 (May): 202–215. https://doi.org/10.1016/j.envres.2019.02.010.
Liu, Q., B. B. Tolar, M. J. Ross, J. B. Cheek, C. M. Sweeney, N. J. Wallsgrove, B. N. Popp, and J. T. Hollibaugh. 2018b. “Light and temperature control the seasonal distribution of thaumarchaeota in the South Atlantic bight.” ISME J. 12 (6): 1473–1485. https://doi.org/10.1038/s41396-018-0066-4.
Logsdon, G. 2010. “Applying treated human biosolids to farmland.” In Holy shit- managingmanure to save mankind, 175, edited by N. Ringer. White River Junction, VT: Chelsea Green Publishing.
Londono, N., A. R. Donovan, H. Shi, M. Geisler, and Y. Liang. 2017. “Impact of TiO2 and ZnO nanoparticles on an aquatic microbial community: Effect at environmentally relevant concentrations.” Nanotoxicology 11 (9–10): 1140–1156. https://doi.org/10.1080/17435390.2017.1401141.
Ma, H., P. L. Williams, and S. A. Diamond. 2013. “Ecotoxicity of manufactured ZnO nanoparticles—A review.” Supplement, Environ. Pollut. 172 (SC): 76–85. https://doi.org/10.1016/j.envpol.2012.08.011.
Ma, R., C. Levard, J. D. Judy, J. M. Unrine, M. Durenkamp, B. Martin, and G. V. Lowry. 2014. “Fate of zinc oxide and silver nanoparticles in a pilot wastewater treatment plant and in processed biosolids.” Environ. Sci. Technol. 48 (1): 104–112. https://doi.org/10.1021/es403646x.
Marambio-Jones, C., and E. M. V. Hoek. 2010. “A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment.” J. Nanopart. Res. 12 (5): 1531–1551. https://doi.org/10.1007/s11051-010-9900-y.
Padmavathy, N., and R. Vijayaraghavan. 2008. “Enhanced bioactivity of ZnO nanoparticles-an antimicrobial study.” Sci. Technol. Adv. Mater. 9 (3): 035004. https://doi.org/10.1088/1468-6996/9/3/035004.
Parsai, T., and A. Kumar. 2019. “Understanding effect of solution chemistry on heteroaggregation of zinc oxide and copper oxide nanoparticles.” Chemosphere 235: 457–469. https://doi.org/10.1016/j.chemosphere.2019.06.171.
Piccinno, F., F. Gottschalk, S. Seeger, and B. Nowack. 2012. “Industrial production quantities and uses of ten engineered nanomaterials in Europe and the world.” J. Nanopart. Res. 14 (9): 1109. https://doi.org/10.1007/s11051-012-1109-9.
Rajput, V. D., T. Minkina, S. Sushkova, V. Tsitsuashvili, S. Mandzhieva, A. Gorovtsov, D. Nevidomskyaya, and N. Gromakova. 2018. “Effect of nanoparticles on crops and soil microbial communities.” J Soils Sediment. 18 (6): 2179–2187. https://doi.org/10.1007/s11368-017-1793-2.
Ray, B., N. Jones, A. C. Manna, and K. T. Ranjit. 2008. “Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms.” FEMS Microbiol. Lett. 279 (1): 71–76. https://doi.org/10.1111/j.1574-6968.2007.01012.x.
Sheng, Z., and Y. Liu. 2017. “Potential impacts of silver nanoparticles on bacteria in the aquatic environment.” J. Environ. Manage. 191 (Apr): 290–296. https://doi.org/10.1016/j.jenvman.2017.01.028.
Stoica, E., and G. J. Herndl. 2007. “Contribution of Crenarchaeota and Euryarchaeota to the prokaryotic plankton in the coastal northwestern Black Sea.” J. Plankton Res. 29 (8): 699–706. https://doi.org/10.1093/plankt/fbm051.
Sun, T. Y., F. Gottschalk, K. Hungerbühler, and B. Nowack. 2014. “Comprehensive probabilistic modelling of environmental emissions of engineered nanomaterials.” Environ. Pollut. 185 (Feb): 69–76. https://doi.org/10.1016/j.envpol.2013.10.004.
Wang, H., A. S. Adeleye, Y. Huang, F. Li, and A. A. Keller. 2015. “Heteroaggregation of nanoparticles with biocolloids and geocolloids.” Adv. Colloid Interface Sci. 226 (Dec): 24–36. https://doi.org/10.1016/j.cis.2015.07.002.
Ward, C. S., J. F. Pan, B. P. Colman, Z. Wang, C. A. Gwin, T. C. Williams, A. Ardis, C. K. Gunsch, and D. E. Hunt. 2019. “Conserved microbial toxicity responses for acute and chronic silver nanoparticle treatments in wetland mesocosms.” Environ. Sci. Technol. 53 (6): 3268–3276. https://doi.org/10.1021/acs.est.8b06654.
Xu, J., X. Luo, Y. Wang, and Y. Feng. 2018. “Evaluation of zinc oxide nanoparticles on lettuce (Lactuca sativa L.) growth and soil bacterial community.” Environ. Sci. Pollut. Res. 25 (6): 6026–6035. https://doi.org/10.1007/s11356-017-0953-7.
You, T., D. Liu, J. Chen, Z. Yang, R. Dou, X. Gao, and L. Wang. 2018. “Effects of metal oxide nanoparticles on soil enzyme activities and bacterial communities in two different soil types.” J. Soils Sediment. 18 (1): 211–221. https://doi.org/10.1007/s11368-017-1716-2.
Zhu, B., L. C. Macleod, T. Kitten, and P. Xu. 2018. “Streptococcus sanguinis biofilm formation & interaction with oral pathogens.” Future Microbiol. 13 (8): 915–932. https://doi.org/10.2217/fmb-2018-0043.
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Published In
Journal of Environmental Engineering
Volume 146 • Issue 7 • July 2020
Copyright
©2020 American Society of Civil Engineers.
History
Received: Aug 17, 2019
Accepted: Jan 30, 2020
Published online: May 8, 2020
Published in print: Jul 1, 2020
Discussion open until: Oct 8, 2020
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