Soil Microcosm Study for Bioremediation by a Crude Oil Degrading Pseudomonas aeruginosa NCIM 5514
Publication: Journal of Environmental Engineering
Volume 146, Issue 5
Abstract
Agricultural land pollution is a big threat globally, which is associated with petroleum industry activities. Hence, the exploration of remedial methods for the treatment of polluted agricultural soils is necessary. In light of this, the biodegradation of petroleum hydrocarbon pollutants is becoming very important. A hydrocarbon degrading Pseudomonas aeruginosa National Collection of Industrial Microorganisms (NCIM) 5514, isolated from crude oil-contaminated soil, has been used for soil microcosm experiments. A bioaugmentation of a contaminated agricultural soil microcosm with P. aeruginosa NCIM 5514 showed petroleum hydrocarbons degradation in 60 days. This study has resulted in the biodegradation of a considerable amount of petroleum by using single bacteria in 60 days, employing agricultural soil microcosms. The dilution plate-counting technique indicated a population of the Pseudomonas strain to be colony forming units per gram () at zero day (just after bioaugmentation), which decreased to at 60 days. The survival of this strain in artificially crude oil contaminated agricultural soil microcosm showed its potential to be used as a bioremediation tool toward farmland restoration.
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Data Availability Statement
All data, models, and code generated or used during the study appear in the published article.
Acknowledgments
The authors acknowledge the support for lab facilities provided at the M. G. Science Institute and Indian Institute of Advanced Research (IIAR), Gandhinagar. We are grateful to the scientists at the Institute of Reservoir Studies (IRS), ONGC, Ahmedabad, for help and support to collect samples and suggestions for the experiment design. Research inputs provided by the late Mahesh C. Sharma, Biotechnology Department, Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, India, are gratefully appreciated.
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Published In
Journal of Environmental Engineering
Volume 146 • Issue 5 • May 2020
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©2020 American Society of Civil Engineers.
History
Received: Apr 23, 2019
Accepted: Oct 14, 2019
Published online: Feb 28, 2020
Published in print: May 1, 2020
Discussion open until: Jul 28, 2020
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