Abstract
This study investigates the use of extracellular polymeric substance (EPS), an eco-friendly and biodegradable bioflocculant, for the treatment of composting leachate [raw leachate (BR9) and biologically treated leachate (BB1)]. The removal of chemical oxygen demand (COD) (69% for BR9 and 92% for BB1), phosphorus (92% for BR9 and 94% for BB1) and ammoniacal nitrogen (65.7% for BR9 and 96% for BB1) was higher using a combination of slime-EPS (S-EPS) and than using slime-EPS alone for both leachates. However, removal of nitrates–nitrites (95% for BR9 and 99% for BB1) and metals (60%–98%) was higher using slime-EPS without the addition of . In all experiments, slime-EPS performed better than broth-EPS (B-EPS). Further optimization of pH (5–9) and incubation time (0.5–12 h) revealed that the maximum removal of COD, phosphate, ammoniacal nitrogen, nitrates–nitrites, and metals was obtained using a slime-EPS concentration of at pH 8 (leachate pH) and 8 h of incubation time. Moreover, the settled solids after EPS treatment produced a slime-EPS of in 72 h, which could be used for the treatment of composting leachate.
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Acknowledgments
Sincere thanks are given to the Natural Sciences and Engineering Research Council of Canada (Grant No. A4984 and Canada Research Chair) for its financial support.
References
Adesoye, A. M., K. Olayinka, O. O. Olukomaiya, and P. O. Iwuchukwu. 2014. “The removal of phosphates from laundry wastewater using alum and ferrous sulphate as coagulants.” Int. J. Innovation Sci. Res. 8 (2): 256–260.
American Water Works Association. 2011. Water quality & treatment: A handbook on drinking water. New York: McGraw-Hill.
Amin, M. M., and M. M. A. Moazzam. 2014. “Advanced oxidation treatment of composting leachate of municipal solid waste by ozone-hydrogen peroxide.” Int. J. Environ. Health Eng. 3 (1): 21. https://doi.org/10.4103/2277-9183.138415.
APHA (American Public Health Association). 1999. Standard methods for the examination of water and wastewater. 20th ed. Washington, DC: APHA.
Arias, M., J. Da Silva-Carballal, L. Garcia-Rio, J. Mejuto, and A. Nunez. 2006. “Retention of phosphorus by iron and aluminum-oxides-coated quartz particles.” J. Colloid Interface Sci. 295 (1): 65–70. https://doi.org/10.1016/j.jcis.2005.08.001.
Bakhshoodeh, R., N. Alavi, M. Majlesi, and P. Paydary. 2017. “Compost leachate treatment by a pilot-scale subsurface horizontal flow constructed wetland.” Ecol. Eng. 105 (Aug): 7–14. https://doi.org/10.1016/j.ecoleng.2017.04.058.
Bondioli, P., and L. Della Bella. 2005. “An alternative spectrophotometric method for the determination of free glycerol in biodiesel.” Eur. J. Lipid Sci. Technol. 107 (3): 153–157. https://doi.org/10.1002/ejlt.200401054.
Çakmakcı, M., and V. S. Özyaka. 2013. “Aerobic composting leachate treatment by the combination of membrane processes.” Waste Manage. Res. 31 (2): 187–193.
Cosa, S., A. M. Ugbenyen, L. V. Mabinya, K. Rumbold, and A. I. Okoh. 2013. “Characterization and flocculation efficiency of a bioflocculant produced by a marine Halobacillus.” Environ. Technol. 34 (18): 2671–2679. https://doi.org/10.1080/09593330.2013.786104.
De Guardia, A., S. Brunet, D. Rogeau, and G. Matejka. 2002. “Fractionation and characterisation of dissolved organic matter from composting green wastes.” Bioresour. Technol. 83 (3): 181–187. https://doi.org/10.1016/S0960-8524(01)00228-0.
Golie, W. M., and S. Upadhyayula. 2017. “An investigation on biosorption of nitrate from water by chitosan based organic-inorganic hybrid biocomposites.” Int. J. Biol. Macromol. 97 (Apr): 489–502. https://doi.org/10.1016/j.ijbiomac.2017.01.066.
Guo, J., Y. Huang, C. Chen, Y. Xiao, J. Chen, and B. Jian. 2017. “Enhanced anaerobically digested swine wastewater treatment by the composite of polyaluminum chloride (PAC) and Bacillus megatherium G106 derived EPS.” Sci. Rep. 7 (1): 8605. https://doi.org/10.1038/s41598-017-09044-0.
Guo, J., A. K. Lau, Y. Zhang, and J. Zhao. 2015. “Characterization and flocculation mechanism of a bioflocculant from potato starch wastewater.” Appl. Microbiol. Biotechnol. 99 (14): 5855–5861. https://doi.org/10.1007/s00253-015-6589-y.
Guo, J., C. Yang, and L. Peng. 2014. “Preparation and characteristics of bacterial polymer using pre-treated sludge from swine wastewater treatment plant.” Bioresour. Technol. 152 (Jan): 490–498. https://doi.org/10.1016/j.biortech.2013.11.037.
Gutiérrez-Miceli, F. A., R. C. García-Gómez, M. A. Oliva-Llaven, J. A. Montes-Molina, and L. Dendooven. 2017. “Vermicomposting leachate as liquid fertilizer for the cultivation of sugarcane (Saccharum sp.).” J. Plant Nutr. 40 (1): 40–49. https://doi.org/10.1080/01904167.2016.1193610.
Ha, J., A. Gélabert, A. M. Spormann, and G. E. Brown Jr. 2010. “Role of extracellular polymeric substances in metal ion complexation on Shewanella oneidensis: Batch uptake, thermodynamic modeling, ATR-FTIR, and EXAFS study.” Geochim. Cosmochim. Acta 74 (1): 1–15. https://doi.org/10.1016/j.gca.2009.06.031.
He, X.-S., B.-D. Xi, Z.-Y. Zhang, R.-T. Gao, W.-B. Tan, D.-Y. Cui, and Y. Yuan. 2015. “Composition, removal, redox, and metal complexation properties of dissolved organic nitrogen in composting leachates.” J. Hazard. Mater. 283 (Feb): 227–233. https://doi.org/10.1016/j.jhazmat.2014.09.027.
Hoornweg, D., and P. Bhada-Tata. 2012. What a waste: A global review of solid waste management. Washington, DC: World Bank.
Hu, Q., N. Chen, C. Feng, and W. Hu. 2015. “Nitrate adsorption from aqueous solution using granular chitosan-Fe3+ complex.” Appl. Surf. Sci. 347 (Aug): 1–9. https://doi.org/10.1016/j.apsusc.2015.04.049.
Kavita, K., V. K. Singh, A. Mishra, and B. Jha. 2014. “Characterisation and anti-biofilm activity of extracellular polymeric substances from Oceanobacillus iheyensis.” Carbohydr. Polym. 101 (Jan): 29–35. https://doi.org/10.1016/j.carbpol.2013.08.099.
Kim, W. K., Y. K. Sung, H. S. Yoo, and J. T. Kim. 2015. “Optimization of coagulation/flocculation for phosphorus removal from activated sludge effluent discharge using an online charge analyzing system titrator (CAST).” J. Ind. Eng. Chem. 21 (Jan): 269–277. https://doi.org/10.1016/j.jiec.2014.02.034.
Klai, N., N. Hoang, S. Yan, R. D. Tyagi, and R. Y. Surampalli. 2015. “Characterization of extracellular polymeric substances (EPS) produced by Cloacibacterium normanense isolated from wastewater sludge for sludge settling and dewatering.” J. Civ. Environ. Eng. 5 (6): 191. https://doi.org/10.4172/2165-784X.1000191.
Krishnan, S., H. Rawindran, C. Sinnathambi, and J. Lim. 2017. “Comparison of various advanced oxidation processes used in remediation of industrial wastewater laden with recalcitrant pollutants.” In Proc., IOP Conf. Series: Materials Science and Engineering, 012089. Bristol, UK: IOP Publishing.
Krogmann, U., and H. Woyczechowski. 2000. “Selected characteristics of leachate, condensate and runoff released during composting of biogenic waste.” Waste Manage. Res. 18 (3): 235–248. https://doi.org/10.1177/0734242X0001800305.
Liu, W., H. Yuan, J. Yang, and B. Li. 2009. “Characterization of bioflocculants from biologically aerated filter backwashed sludge and its application in dying wastewater treatment.” Bioresour. Technol. 100 (9): 2629–2632. https://doi.org/10.1016/j.biortech.2008.12.017.
Ma, Z., J.-J. Qin, C.-X. Liou, L. Zhang, and S. Valiyaveettil. 2012. “Effects of coagulation, pH and mixing conditions on characteristics of flocs in surface water treatment.” In Proc., World Congress on Advances in Civil, Environmental and Material Research, 26–30. Daejeon, Korea: Techno-Press.
Mahvi, A. H., G. K. Feizabadi, M. H. Dehghani, and S. Mazloomi. 2015. “Efficiency of different coagulants in pretreatment of composting plant leachate.” J. Biodivers. Environ. Sci. 6 (6): 21–28.
Malakootian, M., and A. Fatehizadeh. 2010. “Color removal from water by coagulation/caustic soda and lime.” Iran. J. Environ. Health Sci. Eng. 7 (3): 267–272.
More, T., S. Yan, N. Hoang, R. Tyagi, and R. Surampalli. 2012. “Bacterial polymer production using pre-treated sludge as raw material and its flocculation and dewatering potential.” Bioresour. Technol. 121 (Oct): 425–431. https://doi.org/10.1016/j.biortech.2012.06.075.
More, T. T., J. S. S. Yadav, S. Yan, R. D. Tyagi, and R. Y. Surampalli. 2014. “Extracellular polymeric substances of bacteria and their potential environmental applications.” J. Environ. Manage. 144: 1–25. https://doi.org/10.1016/j.jenvman.2014.05.010.
Naveen, B., D. M. Mahapatra, T. Sitharam, P. Sivapullaiah, and T. Ramachandra. 2017. “Physico-chemical and biological characterization of urban municipal landfill leachate.” Environ. Pollut. 220 (Jan): 1–12. https://doi.org/10.1016/j.envpol.2016.09.002.
Ngoc, U. N., and H. Schnitzer. 2009. “Sustainable solutions for solid waste management in Southeast Asian countries.” Waste Manage. 29 (6): 1982–1995. https://doi.org/10.1016/j.wasman.2008.08.031.
Nouha, K., N. Hoang, Y. Song, R. Tyagi, and R. Surampalli. 2016a. “Characterization of extracellular polymeric substances (EPS) produced by Cloacibacterium normanense isolated from wastewater sludge for sludge settling and dewatering.”J. Civ. Environ. Eng. 5: 6. https://doi.org/10.4172/2165-784X.1000191.
Nouha, K., R. S. Kumar, S. Balasubramanian, and R. D. Tyagi. 2017. “Critical review of EPS production, synthesis and composition for sludge flocculation.” J. Environ. Sci. 66 (Apr): 225–245. https://doi.org/10.1016/j.jes.2017.05.020.
Nouha, K., R. S. Kumar, and R. D. Tyagi. 2016b. “Heavy metals removal from wastewater using extracellular polymeric substances produced by Cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods.” Bioresour. Technol. 212 (Jul): 120–129. https://doi.org/10.1016/j.biortech.2016.04.021.
Pan, X., J. Liu, D. Zhang, X. Chen, W. Song, and F. Wu. 2010. “Binding of dicamba to soluble and bound extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence quenching study.” J. Colloid Interface Sci. 345 (2): 442–447. https://doi.org/10.1016/j.jcis.2010.02.011.
Parmar, K. A., S. Prajapati, R. Patel, and Y. Dabhi. 2011. “Effective use of ferrous sulfate and alum as a coagulant in treatment of dairy industry wastewater.” J. Eng. Appl. Sci. 6 (9): 42–45.
Rames, E., A. Roiko, H. Stratton, and J. Macdonald. 2016. “Technical aspects of using human adenovirus as a viral water quality indicator.” Water Res. 96 (Jun): 308–326. https://doi.org/10.1016/j.watres.2016.03.042.
Ramphal, S., and M. Sibiya. 2014. “Optimization of coagulation-flocculation parameters using a photometric dispersion analyser.” Drinking Water Eng. Sci. 7 (2): 73–82. https://doi.org/10.5194/dwes-7-73-2014.
Ranjbari, A., and N. Mokhtarani. 2018. “Post treatment of composting leachate using ZnO nanoparticles immobilized on moving media.” Appl. Catal. B: Environ. 220 (Jan): 211–221. https://doi.org/10.1016/j.apcatb.2017.08.042.
Roy, D., A. Azaïs, S. Benkaraache, P. Drogui, and R. D. Tyagi. 2018. “Composting leachate: Characterization, treatment, and future perspectives.” Rev. Environ. Sci. Biotechnol. 17 (2): 323–349. https://doi.org/10.1007/s11157-018-9462-5.
Ruan, X. D., L. Li, J. X. Liu. 2013. “Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances.” J. Environ. Sci. 25 (5): 916–924.
Subramanian, S. B., S. Yan, R. D. Tyagi, and R. Surampalli. 2010. “Extracellular polymeric substances (EPS) producing bacterial strains of municipal wastewater sludge: Isolation, molecular identification, EPS characterization and performance for sludge settling and dewatering.” Water Res. 44 (7): 2253–2266. https://doi.org/10.1016/j.watres.2009.12.046.
Sun, J., X. Zhang, X. Miao, and J. Zhou. 2012. “Preparation and characteristics of bioflocculants from excess biological sludge.” Bioresour. Technol. 126 (Dec): 362–366. https://doi.org/10.1016/j.biortech.2012.08.042.
Tian, Y., and L. Zheng. 2006. “Functions and behaviors of activated sludge extracellular polymeric substances (EPS): A promising environmental interest.” J. Environ. Sci. 18 (3): 420–427.
Townsend, T. G. 2018. Landfill bioreactor design & operation. New York: Routledge.
Umar, M., H. A. Aziz, and M. S. Yusoff. 2010. “Variability of parameters involved in leachate pollution index and determination of LPI from four landfills in Malaysia.” Int. J. Chem. Eng. 2010: 1–6. https://doi.org/10.1155/2010/747953.
Ummalyma, S. B., A. K. Mathew, A. Pandey, and R. K. Sukumaran. 2016. “Harvesting of microalgal biomass: Efficient method for flocculation through pH modulation.” Bioresour. Technol. 213 (Aug): 216–221. https://doi.org/10.1016/j.biortech.2016.03.114.
USEPA. 2009. National recommended water quality criteria. Washington, DC: USEPA.
Wang, J.-P., Y.-Z. Chen, X.-W. Ge, and H.-Q. Yu. 2007. “Optimization of coagulation-flocculation process for a paper-recycling wastewater treatment using response surface methodology.” Colloids Surf.: Physicochem. Eng. Aspects 302 (1–3): 204–210. https://doi.org/10.1016/j.colsurfa.2007.02.023.
Wang, L. K., N. C. Pereira, and Y.-T. Hung. 2005. Handbook of environmental engineering. Totowa, NJ: Humana Press.
Willie, S., and V. Clancy. 2000. NOAA/NRC intercomparison for nutrients in seawater. Silver Spring, MD: National Oceanic and Atmospheric Administration.
Yang, K., Z. Li, H. Zhang, J. Qian, and G. Chen. 2010. “Municipal wastewater phosphorus removal by coagulation.” Environ. Technol. 31 (6): 601–609. https://doi.org/10.1080/09593330903573223.
Yang, Q., K. Luo, D. x. Liao, X. m. Li, D. b. Wang, X. Liu, G. m. Zeng, and X. Li. 2012. “A novel bioflocculant produced by K lebsiella sp. and its application to sludge dewatering.” Water Environ. J. 26 (4): 560–566. https://doi.org/10.1111/j.1747-6593.2012.00319.x.
Yu, G.-H., P.-J. He, and L.-M. Shao. 2009. “Characteristics of extracellular polymeric substances (EPS) fractions from excess sludges and their effects on bioflocculability.” Bioresour. Technol. 100 (13): 3193–3198. https://doi.org/10.1016/j.biortech.2009.02.009.
Zhao, T., and T. Feng. 2016. “Application of modified chitosan microspheres for nitrate and phosphate adsorption from aqueous solution.” RSC Adv. 6 (93): 90878–90886. https://doi.org/10.1039/C6RA17474D.
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Published In
Journal of Environmental Engineering
Volume 145 • Issue 11 • November 2019
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©2019 American Society of Civil Engineers.
History
Received: Dec 26, 2018
Accepted: Mar 5, 2019
Published online: Aug 26, 2019
Published in print: Nov 1, 2019
Discussion open until: Jan 26, 2020
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