Abstract
To systematically understand the effects of the culturing properties and biodegradation reactions on 4-nitrophenol (PNP)-degrading microorganisms, the bacterium YUP-3 was isolated from soil samples with no PNP-pollution history using a novel method. This bacterium was domesticated to utilize PNP as the sole source of carbon and nitrogen in mineral salt medium. Response surface methodology (RSM) was used to select the most important variables from the simultaneous study on PNP degradation after 48 h incubation. The theoretical maximum PNP degradation of 100.0% was obtained under optimized conditions, and temperature and PNP initial concentrations were concluded to be significant variables. Under carbon- and nitrogen-deficient conditions, five metabolic products of PNP were identified through gas chromatography–mass spectrometry. Hydrogenation and deoxidation were analyzed as two main reactions through PNP degradation to produce hydroquinone. These results demonstrated the importance of the novel PNP degradation pathway under nutrition deficient conditions and the potential use of this highly effective PNP degrading bacterium.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31370421), the Wuhan basic research plan (2014060101010060), and the National Talent Plan of Science Foundation (Wuhan University, J1103409).
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Information & Authors
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Published In
Journal of Environmental Engineering
Volume 144 • Issue 4 • April 2018
Copyright
©2018 American Society of Civil Engineers.
History
Received: Apr 13, 2017
Accepted: Sep 15, 2017
Published online: Jan 17, 2018
Published in print: Apr 1, 2018
Discussion open until: Jun 17, 2018
ASCE Technical Topics:
- Bacteria
- Biological processes
- Chemical compounds
- Chemical degradation
- Chemical elements
- Chemical processes
- Chemicals
- Chemistry
- Environmental engineering
- Geomechanics
- Geotechnical engineering
- Microbes
- Nitrogen
- Nutrient pollution
- Organisms
- Pollutants
- Pollution
- Soil analysis
- Soil mechanics
- Soil properties
- Waste management
- Water pollution
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