Comparative Study of Enterobacter aerogenes and Mixed-Culture Bacteria for Acrylamide Biodegradation in Sequencing Batch Reactor Wastewater-Treatment Systems
Publication: Journal of Environmental Engineering
Volume 144, Issue 3
Abstract
Most recent researches have focused on acrylamide (AM) biodegradation with the pure culture of various microorganisms including Enterobacter aerogenes. In this study, the acrylamide biodegradations from synthetic wastewater containing different acrylamide concentrations by mixed-culture bacteria, E. aerogenes, and a mixture of both bacteria in a sequencing batch reactor (SBR) wastewater-treatment systems controlled at the solid retention time (SRT) of 10 days were comparatively evaluated under aerobic conditions. The results revealed that both bacteria could immediately remove acrylamide in the SBR systems, but E. aerogenes did not biodegrade acrylamide as efficiently as reported in culture media. The growth rate of E. aerogenes was limited at 10 days SRT, resulting in low biomass production and intracellular polyphosphate accumulation, thereby reducing heterotrophic nitrification. However, it was found that E. aerogenes removed acrylamide at higher rate than mixed-culture bacteria in the SBR system. In this study, it is reported that free ammonia nitrogen (FAN), as one product from acrylamide biodegradation, inhibited amidase activities, resulting in lower acrylamide removal efficiencies in the SBR systems after a long-term operation. FAN also inhibited autotrophic nitrification in the SBR system containing mixed-culture bacteria, resulting in FAN accumulation. A mixture of both bacteria enhanced acrylamide biodegradation because simultaneous autotrophic nitrification and heterotrophic nitrification minimized the FAN accumulation.
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Acknowledgments
The authors are grateful for all financial supports from the Research and Development Fund of the Faculty of Engineering, Burapha University (Grant No. 22/2553), the Office of the Higher Education Commission, Thailand, under the program of Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree, and Center of Excellence on Environmental Health and Toxicology (Grant No. ETM-R-05-112551).
References
Anthonisen, A. C., Loehr, R. C., Prakasam, T. B. S., and Srinath, E. G. (1976). “Inhibition of nitrification by ammonia and nitrous acid.” J. Water Pollut. Control Fed., 48(5), 835–852.
BRENDA (Braunschweig Enzyme Database). (2017). “Information on EC 3.5.1.4—Amidase.” ⟨http://www.brenda-enzymes.org/enzyme.php?ecno=3.5.1.4⟩ (Jan. 28, 2017).
Buranasilp, K., and Charoenpanich, J. (2011). “Biodegradation of acrylamide by Enterobacter aerogenes isolated from wastewater in Thailand.” J. Environ. Sci.-China., 23(3), 396–403.
Charoenpanich, J. (2013). Applied bioremediation-active and passive approaches, InTech Open Science Online, Rijeka, Croatia.
Clescerl, L. S., Greenberg, A. E., and Eaton, A. D. (1998). Standard methods for the examination of water and wastewater, American Public Health Association, Washington, DC.
Harold, F. M. (1963). “Accumulation of inorganic polyphosphate in Aerobacter aerogenes. I: Relationship to growth and nucleic acid synthesis.” J. Bacteriol., 86(2), 216–221.
Harold, F. M. (1966). “Inorganic polyphosphates in biology: Structure, metabolism, and function.” Microbiol. Mol. Biol. Rev., 30(4), 772–794.
Hormaeche, E., and Edwards, P. R. (1960). “A proposed genus Enterobacter.” Int. B. Bact. Nomencl. T., 10(2), 71–74.
Junqua, G., Spinelli, S., and Gonzalez, C. (2015). “Occurrence and fate of acrylamide in water-recycling systems and sludge in aggregate industries.” Environ. Sci. Pollut. R., 22(9), 6452–6460.
Kimbara, K., et al. (1989). “Cloning and sequencing of two tandem genes involved in degradation of 2, 3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102.” J. Bacteriol., 171(5), 2740–2747.
Lee, J. K., Choi, C. K., Lee, K. H., and Yim, S. B. (2008). “Mass balance of nitrogen, and estimates of COD, nitrogen and phosphorus used in microbial synthesis as a function of sludge retention time in a sequencing batch reactor system.” Bioresour. Technol., 99(16), 7788–7796.
Lin, Y. M., Tay, J. H., Liu, Y., and Hung, Y. T. (2009). Biological treatment processes, Humana, New York.
Liu, S. (2013). Bioprocess engineering: Kinetics, biosystems, sustainability, and reactor design, Elsevier, Oxford, U.K.
López-Vázquez, C. M., Hooijmans, C. M., Brdjanovic, D., Gijzen, H. J., and van Loosdrecht, M. C. M. (2007). “A practical method for quantification of phosphorus- and glycogen-accumulating organism populations in activated sludge systems.” Water Environ. Res., 79(13), 2487–2498.
McFarland, J. (1907). “Nephelometer: An instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines.” J. Am. Med. Assoc., XLIX(14), 1176–1178.
Nawaz, M. S., Franklin, W., and Cerniglia, C. E. (1993). “Degradation of acrylamide by immobilized cells of a Pseudomonas sp. and Xanthomonas maltophilia.” Can. J. Microbiol., 39(2), 207–212.
Prabu, C. S., and Thatheyus, A. J. (2007). “Biodegradation of acrylamide employing free and immobilized cells of Pseudomonas aeruginosa.” Int. Biodeterior. Biodegrad., 60(2), 69–73.
Riffat, R. (2012). Fundamentals of wastewater treatment and engineering, CRC, Boca Raton, FL.
Robertson, G. P., and Groffman, P. M. (2007). Soil microbiology, ecology and biochemistry, Academic, Burlington, MA.
Scherrard, J. H., and Schroeder, E. D. (1972). “Relationship between the observed cell yield coefficient and mean cell residence time in the completely mixed activated sludge process.” Water Res., 6(9), 1039–1049.
Schomburg, D., and Salzmann, M. (1991). Enzyme handbook 4, class 3: Hydrolases, Springer, New York.
Shanker, R., Ramakrishna, C., and Seth, P. K. (1990). “Microbial degradation of acrylamide monomer.” Arch. Microbiol., 154(2), 192–198.
USEPA. (1979). Aqueous ammonia equilibrium-tabulation of percent un-ionized ammonia, Washington, DC.
USEPA. (2010). Toxicological review of acrylamide, Washington, DC.
van Niel, E. W. J., Arts, P. A. M., Wesselink, B. J., Robertson, L. A., and Kuenen, J. G. (1993). “Competition between heterotrophic and autotrophic nitrifiers for ammonia in chemostat cultures.” FEMS Microbiol. Ecol., 102(2), 109–118.
Vymazal, J., and Kröpfelová, K. (2008). Environmental pollution, Springer, Dordrecht, Netherlands.
Wu, J., He, C., van Loosdrecht, M. C. M., and Pérez, J. (2016). “Selection of ammonium oxidizing bacteria (AOB) over nitrite oxidizing bacteria (NOB) based on conversion rates.” Chem. Eng. J., 304(15), 953–961.
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©2017 American Society of Civil Engineers.
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Received: Apr 26, 2017
Accepted: Sep 1, 2017
Published online: Dec 23, 2017
Published in print: Mar 1, 2018
Discussion open until: May 23, 2018
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