Abstract
Since the inocula for the start-up of the anaerobic reactors (ABs) is still limited in many places, the expansion of the source of feeding anaerobic granular sludge is critical for the application of anaerobic reactors. This paper describes the performance and the bacterial community change during the start-up period of a spiral symmetry stream anaerobic bioreactor (SSSAB) inoculated with four-year storage anaerobic granular sludge. The SSSAB could be successfully started within 58 days, indicating that long-term storage sludge could be an alternative seeding anaerobic reactor. The high-throughput 16S rRNA gene amplicon sequencing using the MiSeq platform was applied to reveal the microbial community change of the granular sludge. The first-time increase of influent chemical oxygen demand (COD) concentration was suggested to be carried out carefully, since the COD removal efficiency would fluctuate sharply if the increment of COD was not appropriate. After start-up, the influent COD concentration of SSSAB was , while the hydraulic retention time was 12 h. The organic loading rate (OLR) reached up to . The most dominant bacterial phyla in the anaerobic granular sludge during the start-up of the SSSAB were Actinobacteria, Bacteroidetes, Proteobacteria, Chloroflexi, and Firmicutes, which accounted for 77.60% of total bacteria on average. Actinobacteria, Chloroflexi, and Proteobacteria played an important role in sludge granulation and growth. Chloroflexi and Firmicutes were deduced to be the key bacteria for the recovery of sludge activity.
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Acknowledgments
The authors would like to thank the National Natural Science Foundation of China (Project No. 51208087). The authors wish to thank the Jiangsu Province Key Laboratory of Environmental Engineering (Grant No. ZX2014001) and the Sichuan Provincial Key Lab of Process Equipment and Control Opening Program (Project No. GK201402).
References
APHA (American Public Health Association). (2005). Standard methods for the examination of water and wastewater, Vol. 21, Water Environment Federation, Alexandria, VA, 258–259.
Ariesyady, H. D., Ito, T., and Okabe, S. (2007). “Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester.” Water Res., 41(7), 1554–1568.
Cardinali-Rezende, J., Colturato, L. F., Colturato, T. D., Chartone-Souza, E., Nascimento, A. M., and Sanz, J. L. (2012). “Prokaryotic diversity and dynamics in a full-scale municipal solid waste anaerobic reactor from start-up to steady-state conditions.” Bioresour. Technol., 119, 373–383.
Chachkhiani, M., et al. (2004). “16S rDNA characterisation of bacterial and archaeal communities during start-up of anaerobic thermophilic digestion of cattle manure.” Bioresour. Technol., 93(3), 227–232.
Chen, J., Tang, C., Zheng, P., and Zhang, L. (2008). “Performance of lab-scale SPAC anaerobic bioreactor with high loading rate.” Chin. J. Biotechnol., 24(8), 1413–1419.
Chen, J. L., Ortiz, R., Steele, T. W., and Stuckey, D. C. (2014). “Toxicants inhibiting anaerobic digestion: A review.” Biotechnol. Adv., 32(8), 1523–1534.
Chen, X., et al. (2015a). “Rheological behaviors of anaerobic granular sludge in a spiral symmetry stream anaerobic bioreactor.” Water Sci. Technol., 72(4), 658–664.
Chen, X., et al. (2015b). “Shock resistance characteristic of a spiral symmetry stream anaerobic bio-reactor.” Water Sci. Technol., 73(4), 916–923.
Chen, X., Dai, R., Xiang, X., and Tang, M. (2013). “Optimum fluidization velocity of granular sludge bed for anaerobic fluidized-bed bioreactors.” J. Chem. Technol. Biotechnol., 88(12), 2133–2140.
Cheon, J., Hidaka, T., Mori, S., Koshikawa, H., and Tsuno, H. (2008). “Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters.” J. Biosci. Bioeng., 106(2), 134–140.
Chong, S., Sen, T. K., Kayaalp, A., and Ang, H. M. (2012). “The performance enhancements of upflow anaerobic sludge blanket (UASB) reactors for domestic sludge treatment—A State-of-the-art review.” Water Res., 46(11), 3434–3470.
Chouari, R., Le Paslier, D., Daegelen, P., Ginestet, P., Weissenbach, J., and Sghir, A. (2005). “Novel predominant archaeal and bacterial groups revealed by molecular analysis of an anaerobic sludge digester.” Environ. Microbiol., 7(8), 1104–1115.
Collins, G., Woods, A., McHugh, S., Carton, M. W., and O’Flaherty, V. (2003). “Microbial community structure and methanogenic activity during start-up of psychrophilic anaerobic digesters treating synthetic industrial wastewaters.” FEMS Microbial. Ecol., 46(2), 159–170.
Dangcong, P., Bernet, N., Delgenes, J.-P., and Moletta, R. (1999). “Aerobic granular sludge—A case report.” Water Res., 33(3), 890–893.
Delafontaine, M., Naveau, H., and Nyns, E.-J. (1979). “Fluorimetric monitoring of methanogenesis in anaerobic digestors.” Biotechnol. Lett., 1(2), 71–74.
Delbès, C., Moletta, R., and Godon, J.-J. (2001). “Bacterial and archaeal 16S rDNA and 16S rRNA dynamics during an acetate crisis in an anaerobic digestor ecosystem.” FEMS Microbial. Ecol., 35(1), 19–26.
Ike, M., et al. (2010). “Microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in Kyoto eco-energy project.” Bioresour. Technol., 101(11), 3952–3957.
Kampmann, K., Ratering, S., Kramer, I., Schmidt, M., Zerr, W., and Schnell, S. (2012). “Unexpected stability of Bacteroidetes and Firmicutes communities in laboratory biogas reactors fed with different defined substrates.” Appl. Environ. Microbiol., 78(7), 2106–2119.
Kwon, S., Kim, T.-S., Yu, G. H., Jung, J.-H., and Park, H.-D. (2010). “Bacterial community composition and diversity of a full-scale integrated fixed-film activated sludge system as investigated by pyrosequencing.” J. Microbiol. Biotechnol., 20(12), 1717–1723.
Lettinga, G., Field, J., Van Lier, J., Zeeman, G., and Huishoff Pol, L. (1997). “Advanced anaerobic wastewater treatment in the near future.” Water Sci. Technol., 35(10), 5–12.
Levén, L., Eriksson, A. R., and Schnürer, A. (2007). “Effect of process temperature on bacterial and archaeal communities in two methanogenic bioreactors treating organic household waste.” FEMS Microbiol. Ecol., 59(3), 683–693.
Liu, W.-T., Chan, O.-C., and Fang, H. H. (2002). “Microbial community dynamics during start-up of acidogenic anaerobic reactors.” Water Res., 36(13), 3203–3210.
Martens, E. C., Chiang, H. C., and Gordon, J. I. (2008). “Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.” Cell Host Microb., 4(5), 447–457.
McCarty, P. L. (2012). Environmental biotechnology: Principles and applications, Tata McGraw-Hill Education, New York City.
Michaud, S., Bernet, N., Buffière, P., Roustan, M., and Moletta, R. (2002). “Methane yield as a monitoring parameter for the start-up of anaerobic fixed film reactors.” Water Res., 36(5), 1385–1391.
Oz, N., Ince, O., Turker, G., and Ince, B. (2012). “Effect of seed sludge microbial community and activity on the performance of anaerobic reactors during the start-up period.” World J. Microbiol. Biotechnol., 28(2), 637–647.
Qu, Y., et al. (2015). “Responses of microbial communities to single-walled carbon nanotubes in phenol wastewater treatment systems.” Environ. Sci. Technol., 49(7), 4627–4635.
R version 3.1.0 [Compter software]. R Core Team, Auckland, New Zealand.
Tang, C.-J., et al. (2011). “Performance of high-loaded ANAMMOX UASB reactors containing granular sludge.” Water Res., 45(1), 135–144.
Weiland, P., and Rozzi, A. (1991). “The start-up, operation and monitoring of high-rate anaerobic treatment systems: discusser’s report.” Water Sci. Technol., 24(8), 257–277.
Yamada, T., Sekiguchi, Y., Imachi, H., Kamagata, Y., Ohashi, A., and Harada, H. (2005). “Diversity, localization, and physiological properties of filamentous microbes belonging to Chloroflexi subphylum I in mesophilic and thermophilic methanogenic sludge granules.” Appl. Environ. Microbiol., 71(11), 7493–7503.
Zakrzewski, M., et al. (2012). “Profiling of the metabolically active community from a production-scale biogas plant by means of high-throughput metatranscriptome sequencing.” J. Biotechnol., 158(4), 248–258.
Zhang, T., Shao, M.-F., and Ye, L. (2011). “454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants.” ISME J., 6(6), 1137–1147.
Zhang, X., et al. (2015). “Illumina MiSeq sequencing reveals diverse microbial communities of activated sludge systems stimulated by different aromatics for indigo biosynthesis from indole.” PLoS One, 10(4), e0125732.
Zhao, Y., Ren, N., and Wang, A. (2008). “Contributions of fermentative acidogenic bacteria and sulfate-reducing bacteria to lactate degradation and sulfate reduction.” Chemosphere, 72(2), 233–242.
Zhu, Y., et al. (2015). “Physicochemical characteristics and microbial community evolution of biofilms during the start-up period in a moving bed biofilm reactor.” Bioresour. Technol., 180, 345–351.
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Published In
Journal of Environmental Engineering
Volume 143 • Issue 9 • September 2017
Copyright
©2017 American Society of Civil Engineers.
History
Received: May 20, 2016
Accepted: Dec 19, 2016
Published online: Apr 8, 2017
Published in print: Sep 1, 2017
Discussion open until: Sep 8, 2017
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