Abstract
The use of chlorine dioxide () instead of free chlorine () as a preoxidant is an effective option for reducing disinfection byproducts (DBPs) and enhancing coagulation in water treatment plants (WTPs). This study takes a first look at water quality and bacterial community responses within a WTP when switching preoxidants between and . Water samples and biofilm coupons inserted in the sedimentation basin were collected during the change between and in a local WTP. Biofilm density and bacteria viability significantly decreased in response to the preoxidant change. Molecular methods confirmed the switch to oxidation that decreased the diversity index for both biofilms and basin waters. Range weighted richness calculations reveal that changing the preoxidant results in a more adverse environment for bacterial growth, compared to maintaining one preoxidant over time. The insights provided regarding microbial growth and diversity under differing oxidants offer a unique perspective in water treatment that warrants further examination.
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Acknowledgments
The authors would like to thank the Beaver Water District for providing funding for this project and allowing access within the water treatment plant. Additional thanks to the Arkansas Water Resources Center for supplemental financial support. Confocal images were obtained on an instrument provided by the P3 Center, funded through the Arkansas ASSET Initiative II (EPS-1003970) by the National Science Foundation and the Arkansas Science and Technology Authority.
References
APHA (American Public Health Association). (2005). “Standard methods for the examination of water and wastewater.” American Water Works Association, and Water Environment Federation, Washington, DC.
Benarde, M. A., Israel, B. M., Olivieri, V. P., and Granstrom, M. L. (1965). “Efficiency of chlorine dioxide as a bactericide.” Appl. Microbiol., 13(5), 776–780.
Bereschenko, L. A., Heilig, G. H., Nederlof, M. M., van Loosdrecht, M. C., Stams, A. J., and Euverink, G. J. (2008). “Molecular characterization of the bacterial communities in the different compartments of a full-scale reverse-osmosis water purification plant.” Appl. Environ. Microbiol., 74(17), 5297–5304.
Berg, J. D., Hoff, J. C., Roberts, P. V., and Matin, A. (1988). “Resistance of bacterial subpopulations to disinfection by chlorine dioxide.” J. Am. Water Works Assoc., 80(9), 115–119.
Boon, N., Windt, W., Verstraete, W., and Top, E. M. (2002). “Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16 S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants.” FEMS Microbiol. Ecol., 39(2), 101–112.
Carbonero, F., Oakley, B. B., and Purdy, K. J. (2014). “Metabolic flexibility as a major predictor of spatial distribution in microbial communities.” PLoS One, 9(1), e85105.
Eichler, S., et al. (2006). “Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16 S rRNA gene fingerprinting.” Appl. Environ. Microbiol., 72(3), 1858–1872.
Figueiredo, H. C. P., et al. (2005). “Isolation and characterization of strains of Flavobacterium columnare from Brazil.” J. Fish Dis., 28(4), 199–204.
GelCompar II version 5.1 [Computer software]. Applied Maths, Austin, TX.
Georgiades, K., Madoui, M.-A, Le, P., Robert, C., and Raoult, D. (2011). “Phylogenomic analysis of Odyssella thessalonicensis fortifies the common origin of Rickettsiales, Pelagibacter ubique and Reclimonas americana mitochondrion.” PLoS One, 6(9), e24857.
Guglielmi, G., Cohen-Bazire, G., and Bryant, D. A. (1981). “The structure of Gloeobacter violaceus and its phycobilisomes.” Arch. Microbiol., 129(3), 181–189.
Hoefel, D., Monis, P., Grooby, W., Andrews, S., and Saint, C. (2005). “Profiling bacterial survival through a water treatment process and subsequent distribution system.” J. Appl. Microbiol., 99(1), 175–186.
Holinger, E. P., Ross, K. A., Robertson, C. E., Stevens, M. J., Harris, J. K., and Pace, N. R. (2014). “Molecular analysis of point-of-use municipal drinking water microbiology.” Water Res., 49, 225–235.
Hwang, H. G., Kim, M. S., Shin, S. M., and Hwang, C. W. (2014). “Risk assessment of the schmutzdecke of biosand filters: Identification of an opportunistic pathogen in schmutzdecke developed by an unsafe water source.” Int. J. Environ. Res. Public Health, 11(2), 2033–2048.
Johnston, M. D., Simons, E.-A., and Lambert, R. J. W. (2000). “One explanation for the variability of the bacterial suspension test.” J. Appl. Microbiol., 88(2), 237–242.
Linder, K., Lew, J., Carter, B., and Brauer, R. (2006). “Avoiding chlorite: Chlorine and ClO 2 together form fewer DBPs (PDF).” Opflow, 32(8), 24–26.
Lyautey, E., Lacoste, B., Ten-Hage, L., Rols, J.-L., and Garabetian, F. (2005). “Analysis of bacterial diversity in river biofilms using 16 S rDNA PCR-DGGE: Methodological settings and fingerprints interpretation.” Water Res., 39(2), 380–388.
Maeda, S., and Omata, T. (1997). “Substrate-binding lipoprotein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in the transport of nitrate and nitrite.” J. Biol. Chem., 272(5), 3036–3041.
Martiny, A. C., Albrechtsen, H. J., Arvin, E., and Molin, S. (2005). “Identification of bacteria in biofilm and bulk water samples from a nonchlorinated model drinking water distribution system: Detection of a large nitrite-oxidizing population associated with Nitrospira spp.” Appl. Environ. Microbiol., 71(12), 8611–8617.
Marzorati, M., Wittebolle, L., Boon, N., Daffonchio, D., and Verstraete, W. (2008). “How to get more out of molecular fingerprints: Practical tools for microbial ecology.” Environ. Microbiol., 10(6), 1571–1581.
Muyzer, G., de Waal, E. C., and Uitterlinden, A. G. (1993). “Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16 S rRNA.” Appl. Environ. Microbiol., 59(3), 695–700.
Muyzer, G., and Smalla, K. (1998). “Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.” Antonie van Leeuwenhoek, 73(1), 127–141.
Norton, C. D., and LeChevallier, M. W. (2000). “A pilot study of bacteriological population changes through potable water treatment and distribution.” Appl. Environ. Microbiol., 66(1), 268–276.
Pinto, A. J., Xi, C., and Raskin, L. (2012). “Bacterial community structure in the drinking water microbiome is governed by filtration processes.” Environ. Sci. Technol., 46(16), 8851–8859.
Pressman, J. G., et al. (2010). “Concentration, chlorination, and chemical analysis of drinking water for disinfection byproduct mixtures health effects research: U.S. EPA’s four lab study.” Environ. Sci. Technol., 44(19), 7184–7192.
Rittmann, D. D. (1997). “‘Can you have your cake and eat it too’ with chlorine dioxide?” Water Eng. Manage., 144(4), 30–35.
Rook, J. J. (1976). “Haloforms in drinking water.” J. Am. Water Works Assoc., 68(3), 168–172.
SAS/IML version 9.22 [Computer software]. SAS Institute, Cary, NC.
Selmi, C., et al. (2003). “Patients with primary biliary cirrhosis react against a ubiquitous xenobiotic-metabolizing bacterium.” Hepatology, 38(5), 1250–1257.
Sigler, W., Miniaci, C., and Zeyer, J. (2004). “Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure.” J. Microbiol. Methods, 57(1), 17–22.
Smyk, D. S., Rigopoulou, E. I., and Bogdanos, D. P. (2013). “Potential roles for infectious agents in the pathophysiology of primary biliary cirrhosis: What’s new?” Curr. Infect. Dis. Rep., 15(1), 14–24.
Tucker, M. P., Mohagheghi, A., Grohmann, K., and Himmel, M. E. (1989). “Ultra-thermostable cellulases from Acidothermus cellulolyticus: Comparison of temperature optima with previously reported cellulases.” Nat. Biotechnol., 7(8), 817–820.
U.S. Environmental Protection Agency Office of Water. (1999). “Alternative disinfectants and oxidants guidance manual.” Washington, DC.
Vaz-Moreira, I., Egas, C., Nunes, O. C., and Manaia, C. M. (2013). “Bacterial diversity from the source to the tap: A comparative study based on 16 S rRNA gene-DGGE and culture-dependent methods.” FEMS Microbiol. Ecol., 83(2), 361–374.
Wang, Z., Kim, J., and Seo, Y. (2012). “Influence of bacterial extracellular polymeric substances on the formation of carbonaceous and nitrogenous disinfection byproducts.” Environ. Sci. Technol., 46(20), 11361–11369.
Yang, X., Guo, W., and Lee, W. (2013a). “Formation of disinfection byproducts upon chlorine dioxide preoxidation followed by chlorination or chloramination of natural organic matter.” Chemosphere, 91(11), 1477–1485.
Yang, X., Guo, W., Zhang, X., Chen, F., Ye, T., and Liu, W. (2013b). “Formation of disinfection by-products after pre-oxidation with chlorine dioxide or ferrate.” Water Res., 47(15), 5856–5864.
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© 2015 American Society of Civil Engineers.
History
Received: Apr 2, 2015
Accepted: Aug 5, 2015
Published online: Oct 16, 2015
Published in print: Feb 1, 2016
Discussion open until: Mar 16, 2016
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