Cellulosic Fermentation Using Bacillus thermoamylovorans–Enabled Digested Sludge
Publication: Journal of Environmental Engineering
Volume 141, Issue 12
Abstract
The effectiveness of the Bacillus thermoamylovorans-enabled digested sludge in catalyzing cellulosic fermentation was tested under batch, mesophilic (40 plus or minus 1°C), unacclimatized, and high-loading (1.6 g cellulose in 80 mL aqueous solution) conditions. B. thermoamylovorans isolated from a molasses-fed anaerobic bioreactor was capable of quickly converting cellulose to alcohols and volatile organic acids (VOAs) at an overall rate of VSS-day [volatile suspended solids (VSS), soluble chemical oxygen demand ()] without producing . The digested sludge grown on pig manure, after an initial lag of 6 days, was able to produce , alcohols, and VOAs from cellulose at the rates of VSS-day and VSS-day, respectively. The experiment which used a mixture of 0.014 g B. thermoamylovorans and 0.172 g digested sludge yielded the best results, with about 11-fold and sevenfold rate increases in production and acid fermentation, respectively, compared to the digested sludge. Moreover, the initial lag of the B. thermoamylovorans-enabled digested sludge was reduced to about 3.0 days. In addition to , the main end products included ethanol (), acetic acid (), and butyric acid (). Under the conditions tested, the presence of cooperative interactions between B. thermoamylovorans and digested sludge was evident, provided that the mass of the former was small relative to that of the latter in the mixture cultures (i.e., 3–8%).
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Acknowledgments
This project is financially supported by the Environmental Protection Administration of the Republic of China (EPA-95-U1U4-007).
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Published In
Journal of Environmental Engineering
Volume 141 • Issue 12 • December 2015
Copyright
© 2015 American Society of Civil Engineers.
History
Received: May 16, 2014
Accepted: May 20, 2015
Published online: Jul 9, 2015
Published in print: Dec 1, 2015
Discussion open until: Dec 9, 2015
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