Determination of Porosity and Thickness of Biofilm Attached on Irregular-Shaped Media
Publication: Journal of Environmental Engineering
Volume 139, Issue 7
Abstract
Biofilm density, porosity, and thickness are biofilm architecture properties that are important but often difficult to measure. In this study, wet and dry biofilm densities and biofilm porosity in shredded tire biofilters were measured using a volumetric displacement method and a new porosity equation. Methods for determining the surface area and mean thickness of biofilms attached to shredded tires were developed on the basis of the box- and ellipsoid-shape models with the data of (1) the volume calculated from the measured weight of the filter medium () or (2) the three longest lines of the filter medium measured with a digital caliper. As a benchmark, the surface area of the shredded tire particles were calculated from X-ray computed tomography (CT) scanning and compared to, and linearly correlated with, the results from the models. The ellipsoid-shape model with the modified length-width-depth data and was determined to be the best model to compute the surface area and biofilm thickness. The method of biofilm thickness measurement may be applicable in attached growth systems packed with other irregular-shaped media.
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Acknowledgments
The authors would like to thank the University of Nebraska-Lincoln, especially the Nebraska Center for Energy Sciences Research for funding the study, along with the USEPA Project Advanced Decentralized Water/Energy Network Design for Sustainable Infrastructure (EPA #: CR-83419301) and the NIST Project Innovative Measurement Science for Shape Metrology.
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Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 139 • Issue 7 • July 2013
Pages: 923 - 931
Copyright
© 2013 American Society of Civil Engineers.
History
Received: May 22, 2012
Accepted: Jan 29, 2013
Published online: Jan 31, 2013
Published in print: Jul 1, 2013
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