Evaluation of Enhanced Reductive Dechlorination of Trichloroethylene Using Gene Analysis: Pilot-Scale Study
Publication: Journal of Environmental Engineering
Volume 139, Issue 3
Abstract
The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated compounds found in groundwater contamination. The objective of this pilot-scale study was to evaluate the effectiveness of an in situ reductive dechlorination process to remediate TCE-contaminated groundwater at a TCE-spill site using specific gene analysis. An injection well was installed inside the TCE plume for substrate and inocula injection. Brown sugar and anaerobic activated sludge (collected from an industrial wastewater-treatment plant with influent containing TCE) as electron donor (primary substrate) and inocula, respectively, were injected to enhance the TCE biodegradation rate through anaerobic reductive dechlorination. Three monitor wells were installed downgradient of the injection well along the groundwater flow path to monitor the TCE degradation trend. Polymerase chain reaction (PCR) analytical results reveal that the major TCE degrader (Dehalococcoides) and TCE-degrading genes (vcrA and tceA) were not observed in site groundwater before sludge injection. Thus, anaerobic sludge was injected for inoculation. After sludge injection, Dehalococcoides and the vcrA and tceA genes were detected in the injection and downgradient monitoring wells. Results show that brown sugar could be fermented to volatile fatty acids (VFAs), which could be used as substrates to enhance TCE reductive dechlorination. Up to 97% of TCE removal was observed after 204 days of operation. This reveals that appropriate substrates and inocula are required to effectively enhance the anaerobic reductive dechlorinating rate of TCE. Results indicate that the enhanced in situ bioremediation is a promising technology to remediate TCE-contaminated groundwater, and gene analysis is a necessity to evaluate the feasibility and occurrence of enhanced bioremediation.
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Acknowledgments
This project was funded in part by National Science Council in Taiwan. The authors would like to thank the personnel at the Department of Biological Sciences of National Sun Yat-Sen University and Guan Cheng Environment Technology Protection Co. Ltd. for their assistance and support throughout this project.
References
American Public Health Association (APHA). (2005). Standard methods for the examination of water and waste water, 22nd Ed., APHA-AWWA-WPCF, Washington, DC.
Aulenta, F., Potalivo, M., Majone, M., Papini, M. P., and Tandoi, V. (2006). “Anaerobic bioremediation of groundwater containing a mixture of 1,1,2,2-tetrachloroethane and chloroethenes.” Biodegradation, 17(3), 193–206.
Azizian, M. F., Istok, J. D., and Semprini, L. (2007). “Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push-pull tests.” J. Contam. Hydrol., 90(1–2), 105–124.
Borden, R. C. (2007). “Effective distribution of emulsified edible oil for enhanced anaerobic bioremediation.” J. Contam. Hydrol., 94(1–2), 1–12.
Brar, S. K., et al. (2006). “Bioremediation of hazardous wastes—a review.” Pract. Period. Hazard., Toxic, Radioact. Waste Manage., 10(2), 59–72.
Chen, K. F., Kao, C. M., Chen, T. Y., Weng, C. H., and Tsai, C. T. (2006). “Intrinsic bioremediation of MTBE-contaminated groundwater at a petroleum-hydrocarbon spill site.” Environ. Geol., 50(3), 439–445.
Chen, K. F., Kao, C. M., Hsieh, C. Y., Chen, S. C., and Chen, Y. L. (2005). “Natural biodegradation of MTBE under different environmental conditions: Microcosm and microbial identification studies.” Bull. Environ. Contam. Toxicol., 74(2), 356–364.
Chen, K. F., Yeh, T. Y., Kao, C. M., Sung, W. P., and Lin, C. C. (2012). “Application of nanoscale zero-valent iron (nZVI) to enhance microbial reductive dechlorination of TCE: A feasibility study.” Curr. Nanosci., 8(1), 55–59.
Chen, Y., Lin, T., Huang, C., and Lin, J. (2008). “Cometabolic degradation kinetics of TCE and phenol by Pseudomonas putida.” Chemosphere, 72(11), 1671–1680.
Chien, H. Y., Kao, C. M., Surampalli, R. Y., Huang, W. Y., and Hou, F. (2011). “Development of a four-phase remedial scheme to cleanup petroleum-hydrocarbon contaminated soils.” J. Environ. Eng., 137(7), 602–610.
Coleman, N. V., Bui, N. B., and Holmes, A. J. (2006). “Soluble di-iron monooxygenase gene diversity in soils, sediments and ethene enrichments.” Environ. Microbiol., 8(7), 1228–1239.
EPA Method 601. “Methods for Organic Chemical Analysis of Municipal and Industrial Wastewater, Method 601 – Purgeable Halocarbons.” 600 Series Method, CleanWater Act, Environmental Protection Agency, Washington, DC.
Ha, C., et al. (2010). “Natural gradient drift tests for assessing the feasibility of in situ aerobic cometabolism of trichloroethylene and evaluating the microbial community change.” Water, Air, Soil Pollut., 17, 35–42.
Han, Y. L., Kuo, M. C., Tseng, I. C., and Lu, C. J. (2007). “Semicontinuous microcosm study of aerobic cometabolism of trichloroethylene using toluene.” J. Hazard. Mater., 148(3), 583–591.
Hazen, T. C., et al. (2009). “Use of gene probes to assess the impact and effectiveness of aerobic in situ bioremediation of TCE.” Arch. Microbiol., 191(3), 221–232.
He, J., Ritalahti, K. M., Aiello, M. R., and Löffler, F. E. (2003). “Complete detoxification of vinyl chloride by an anaerobic enrichment culture and identification of the reductively dechlorinating population as a Dehalococcoides species.” Appl. Environ. Microbiol., 69(2), 996–1003.
Holmes, V. F., He, J., He, J., Lee, P. K. H., and Alvarez-Cohen, L. (2006). “Discrimination of multiple Dehalococcoides strains in a trichloroethene enrichment by quantification of their reductive dehalogenase genes.” Appl. Environ. Microbiol., 72(9), 5877–5883.
Jang, W., and Aral, M. M. (2008). “Effect of biotransformation on multispecies plume evolution and natural attenuation.” Transp. Porous Med., 72(2), 207–226.
Jiang, H., et al. (2010). “Methanotrophs: Multifunctional bacteria with promising applications in environmental bioengineering.” Biochem. Eng. J., 49(3), 277–288.
Kao, C. M., Chen, C. Y., Chen, S. C., and Chen, Y. L. (2008). “Application of in situ biosparging to remediate a petroleum-hydrocarbon spill site: Field and microbial evaluation.” Chemosphere, 70(8), 1492–1499.
Kao, C. M., Chen, S. S., Chen, S. C., and Chen, Y. L. (2010a). “Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume.” J. Hazard. Mater., 178(1–3), 409–416.
Kao, C. M., Chen, Y. L., Chen, S. C., Yeh, T. Y., and Wu, W. S. (2003). “Enhanced PCE dechlorination by biobarrier systems under different redox conditions.” Water Resour., 37(20), 4885–4894.
Kao, C. M., Chien, H. Y., Surampalli, R. Y., Chien, C. C., and Chen, C. Y. (2010b). “Assessing of natural attenuation and intrinsic bioremediation rates at a petroleum-hydrocarbon spill site: Laboratory and field studies.” J. Environ. Eng., 136(1), 54–67.
Kocamemi, B. A., and Çeçen, F. (2005). “Cometabolic degradation of TCE in enriched nitrifying batch systems.” J. Hazard. Mater., 125(1–3), 260–265.
Lai, K. C. K., Surampalli, R. Y., Tyagi, R. D., Lo, I. M. C., and Yan, S. (2007). “Performance monitoring of remediation technologies for soil and groundwater contamination: Review.” Pract. Period. Hazard., Toxic, Radioact. Waste Manage., 11(3), 132–157.
Lee, M. H., Clingenpeel, S. C., Leiser, O. P., Wymore, R. A., Sorenson, K. S., and Watwood, M. E. (2008). “Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site.” Environ. Pollut., 153(1), 238–246.
Lee, P. K. H., Johnson, D. R., Holmes, V. F., He, J., and Alvarez-Cohen, L. (2006). “Reductive dehalogenase gene expression as a biomarker for physiological activity of Dehalococcoides spp.” Appl. Environ. Microbiol., 72(9), 6161–6168.
Lee, S. (2007). “Enhanced dissolution of TCE in NAPL by TCE-degrading bacteria in wetland soils.” J. Hazard. Mater., 145(1–2), 17–22.
Magnuson, J. K., Romine, M. F., Burris, D. R., and Kingsley, M. T. (2000). “Trichloroethene reductive dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and substrate range characterization.” Appl. Environ. Microbiol., 66(12), 5141–5147.
Morono, Y., Unno, H., Tanji, Y., and Hori, K. (2004). “Addition of aromatic substrates restores trichloroethylene degradation activity in Pseudomonas putida F1.” Appl. Environ. Microbiol., 70(5), 2830–2835.
Révész, S., et al. (2006). “Bacterial community changes in TCE biodegradation detected in microcosm experiments.” Int. Biodeterior. Biodegrad., 58(3–4), 239–247.
Ritalahti, K. M., Amos, B. K., Sung, Y., Wu, Q., Koenigsberg, S. S., and Löffler, F. E. (2006). “Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains.” Appl. Environ. Microbiol., 72(4), 2765–2774.
Tao, Y., Fishman, A., Bentley, W. E., and Wood, T. K. (2004). “Oxidation of benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by toluene 4-monooxygenase of Pseudomonas mendocina KR1 and toluene 3-monooxygenase of Ralstonia pickettii PKO1.” Appl. Environ. Microbiol., 70(7), 3814–3820.
Tartakovsky, B., Manuel, M., and Guiot, S. R. (2005). “Degradation of trichloroethylene in a coupled anaerobic–aerobic bioreactor: Modeling and experiment.” Biochem. Eng. J., 26(1), 72–81.
Tsai, T. T., Kao, C. M., Surampalli, R. Y., and Weng, C. H. (2009). “Enhanced bioremediation of fuel-oil contaminated soils: A laboratory feasibility study.” J. Environ. Eng., 135(9), 845–853.
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Information
Published In
Journal of Environmental Engineering
Volume 139 • Issue 3 • March 2013
Pages: 428 - 437
Copyright
© 2013 American Society of Civil Engineers.
History
Received: May 21, 2012
Accepted: Sep 11, 2012
Published online: Sep 13, 2012
Published in print: Mar 1, 2013
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