Role of Reactive Oxygen Species in Determining Nitrification Inhibition by Metallic/Oxide Nanoparticles
Publication: Journal of Environmental Engineering
Volume 135, Issue 12
Abstract
The toxicity of several metallic/oxide nanoparticles ( , CuO, ZnO, and Ag) to nitrifying bacteria was evaluated individually or in combination in batch studies. Except for the mixture of ZnO and Ag, the mixture inhibition of nanoparticles was roughly the sum of individual inhibition among the nanoparticles studied. Although there was no inhibitory effect of nanoparticles under ambient light or dark conditions, nitrification inhibition was significantly increased when nanoparticles were exposed to ultraviolet (UV) for 30 min. Under UV exposure, both nanoparticles (anatase) and bulk amorphous generated the same amount of reactive oxygen species (ROS) in the bacterial cell although nanoparticles were more toxic than the bulk counterpart. While the inhibition was well correlated to intracellular ROS concentration, the ROS correlations were different for the different forms of or for the different nanoparticles (e.g., Ag versus ). ROS is therefore not a good chemical marker to indicate the toxicity of common metallic/oxide nanoparticles.
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Acknowledgments
This research work was supported by the University of Missouri Research Board and the National Science Foundation under Grant No. NSF0650943. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the writer(s) and do not necessarily reflect the views of the National Science Foundation.
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Information
Published In
Journal of Environmental Engineering
Volume 135 • Issue 12 • December 2009
Pages: 1365 - 1370
Copyright
© 2009 ASCE.
History
Received: Dec 30, 2008
Accepted: May 11, 2009
Published online: May 13, 2009
Published in print: Dec 2009
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