Mixed Culture Acclimatization and Biodegradation of Chlorophenols in Shake Flasks: Effect of the Inoculum Source
Publication: Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 13, Issue 1
Abstract
Shake flask reactors with different inocula were run for the treatment of chlorophenols present in wastewaters. The three different inocula were obtained from—sewage treatment plant, refinery effluent treatment plant (ETP), and paper mill ETP, respectively. The model compounds tested were 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP). The three inocula have different levels of exposure to phenolic compounds. As the paper mill effluent has high level of phenolic compounds, and sewage has no phenolic compound, the inoculum obtained from paper mill ETP would have been acclimatized to some degree to phenolic environment. In our lab, the inocula were acclimatized to synthetic 4-CP and 2,4-DCP for several weeks at in shake flasks. It was found that each inoculum demonstrated different specific activity in chlorophenols degradation. The inoculum from paper mill ETP yielded highest degradation rate for both 4-CP and 2,4-DCP. The effect of a biogenic substrate (glucose) concentration on the acclimatization of chlorophenols was also investigated. Glucose concentrations used in shake flasks were 1, 0.5, and 0%. It was observed that cultures acclimatized with 1% glucose concentration had shown maximum degradation rate. Degradation rate for 2,4-DCP was lower than that for 4-CP. The presence of greater number of chlorine molecules in 2,4-DCP must have contributed to higher toxicity level for the microorganisms. A mixed system comprising of 4-CP and 2,4-DCP was found to be more toxic compared to that obtained in an individual system.
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References
Armenante, P. M., Kafkewitz, D., Lewandowski, G. A., and Jou, C. J. (1999). “Anaerobic-aerobic treatment of halogenated phenolic compounds.” Water Res., 33(3), 681–692.
Azad, S. A., Vikineswary, S., Chong, V. C., and Ramachandran, K. B. (2003). “Rhodovulum sulfidophilum in the treatment and utilization of sardine processing wastewater.” Lett. Appl. Microbiol., 38(1), 13–18.
Bae, H. S., Lee, J. M., and Lee, S. T. (1997). “Biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.” J. Gen. Appl. Microbiol., 43(1), 97–103.
Bagby, M. M., and Sherrard, J. H. (1981). “Combined effects of cadmium and nickel on the activated sludge process.” J. Water Pollut. Control Fed., 53(11), 1609–1619.
Baughman, G. L., Paris, D. F., and Steen, W. C. (1980). “Quantitative expression of biotransformation rate.” Biotransformation and fate of chemicals in the aquatic environment, A. W. Maki, K. L. Dickson, and J. Cairns, Jr., eds., American Society for Microbiology, Washington, D.C., 105–111.
Contrerasa, S., Rodriguez, M., Al Momania, F., Sansa, C., and Esplugasa, S. (2003). “Contribution of the ozonation pre-treatment to the biodegradation of aqueous solutions of 2,4-dichlorophenol.” Water Res., 37(13), 3164–3171.
Goi, A., Trapido, M., and Tuhkanen, T. (2004). “A study of toxicity, biodegradability, and some by-products of ozonised nitrophenols.” Adv. Environ. Res., 8(3–4), 304–311.
Hakulinen, R., Woods, S., Ferguson, J., and Benjamin, M. (1985). “The role of facultative anaerobic microorganisms in anaerobic biodegradation of chlorophenols.” Water Sci. Technol., 17(1), 289–301.
Hu, Z., Ferraina, R. A., Ericson, J. F., and Smets, B. F. (2005). “Effect of long-term exposure, biogenic substrate presence, and electron acceptor conditions on the biodegradation of multiple substituted benzoates and phenolates.” Water Res., 39(15), 3501–3510.
Kim, M. H., and Oliver, J. H. (1999). “Cometabolic degradation of chlorophenols by acinetobacter species.” Water Res., 33(2), 562–574.
Kishino, T., and Kobayashi, K. (1994). “Relationship between the chemical structures of chlorophenols and their dissociation constants and partition constants in several solvent-water systems.” Water Res., 28(7), 1547–1552.
Kulkarni, M., and Chaudhari, A. (2006). “Biodegradation of -nitrophenol by . putida.” Bioresour. Technol., 97(8), 982–988.
Pignatello, M. M., Martinson, J. G., Steiert, R. E., and Crawford, R. L. (1983). “Biodegradation and photolysis of pentachlorophenol in artificial freshwater streams.” Appl. Environ. Microbiol., 46(5), 1024–1031.
Quan, X., Yang, Z., Shi, H., Tang, Q., and Qian, Y. (2005). “The effect of a secondary chlorophenol presence on the removal of 2,4-dichlorophenol (2,4-DCP) in an activated sludge system bioaugmented with 2,4-DCP degrading special culture.” Process Biochem. (Oxford, U.K.), 40(15), 3462–3467.
Sahinkaya, E., and Dilek, F. B. (2006). “Effect of biogenic substrate concentration on the performance of sequencing batch reactor treating 4-CP and 2,4-DCP mixtures.” J. Hazard. Mater., 128(2–3), 258–264.
Scott, J. P., and Ollis, D. F. (1995). “Integration of chemical and biological oxidation processes for water treatment: Review and recommendations.” Environ. Prog., 14(2), 88–103.
Suidan, M. T., Flora, J. R. V., Boyer, T. K., Wuellner, A. M., and Narayanan, B. (1996). “Anaerobic dechlorination using a fluidized-bed GAC reactor.” Water Res., 30(1), 160–170.
Wakelin, N. G., and Forster, C. F. (1998). “The aerobic treatment of grease-containing fast food restaurant wastewaters.” Process Saf. Environ. Prot., 76(B1), 55–61.
Wiggings, B. A., Jones, S. H., and Alexander, M. A. (1987). “Explanations for the acclimation period preceding the mineralization of organic chemicals in aquatic environments.” Appl. Environ. Microbiol., 53(4), 791–796.
Ye, F. X., and Shen, D. S. (2004). “Acclimation of anaerobic sludge degrading chlorophenols and the biodegradation kinetics during acclimation period.” Chemosphere, 54(10), 1573–1584.
Zahn, J. (1993). “Mixed culture biodegradation of pentachlorophenol, hexachlorobenzene, and tetrachloromethane under anaerobic conditions.” Thesis. New Jersey Institute of Technology, Newark, N.J.
Zeikus, G. J., and Johnson, E. A. (1991). Mixed cultures in biotechnology, McGraw-Hill, New York.
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Published In
Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 13 • Issue 1 • January 2009
Pages: 29 - 34
Copyright
© 2009 ASCE.
History
Received: Oct 11, 2007
Accepted: Oct 11, 2007
Published online: Jan 1, 2009
Published in print: Jan 2009
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