Growth of Four Serovar of Bacillus thuringiensis (Var. Kurstaki, Israelensis, Tenebrionis, and Aizawai) in Wastewater Sludge
Publication: Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 11, Issue 2
Abstract
Growth patterns of four different serovar of Bacillus thuringiensis (Bt) (Bt kurstaki, Bt israelensis, Bt tenebrionis, and Bt aizawai) were studied using wastewater sludge as a raw material. A comparative study using synthetic medium (soy) was also performed. It was observed that the four Bt strains gave cell counts of the order of in wastewater sludge and in synthetic medium. The sporulation (% spores) in two media was 90%. The entomotoxicity per unit spore (specific entomotoxicity) was much higher in sludge medium compared to synthetic medium. Therefore, sludge was a good source of nutrients for growth and toxin production by all types of serovar used in this study.
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Acknowledgments
The writers sincerely thank the Natural Sciences and Engineering Research Council of Canada (Grant Nos. A4984, STR 202047, and SCF 192190-96) for financial support.
References
Adams, T. T., Eiteman, M. A., and Adang, M. J. (1999). “Bacillus thuringiensis subsp. kurstaki spore production in batch culture using broiler litter extracts as complex medium.” Bioresour. Technol., 67, 83–87.
American Public Health Association, American Water Works Association, and Water Environment Federation (APHA/AWWA/WEF). (1998). Standard methods for the examination of water and wastewater, APHA, Washington, D.C.
Avignone-Rosa, C., and Mignone, C. F. (1995). “Bacillus thuringiensis growth and toxicity, basic and applied considerations.” Mol. Biotechnol., 4, 55–71.
Barnabé, S. (2000). “Utilisation des boues d’épuration comme substrat pour la production de biopesticide: Induction abiotique de la sporulation chez Bacillus thuringiensis.” INRS–Eau, Québec.
Bernhard, K., and Utz, R. (1993). “Production of Bacillus thuringiensis insecticides for experimental and commercial uses.” Bacillus thuringiensis an environmental biopesticide: Theory and practice, E. Entwistle, J. S. Cory, M. J. Baley, and S. Higgs, eds., Wiley, New York, 255–267.
Bulla, L. A., Bechtel, D. B., Kramer, K. J., Shethna, Y. I., Aronson, A. I., and Fitz-James, P. C. (1980). “Ultrastructure, physiology, and biochemistry of Bacillus thuringiensis.” Crit. Rev. Microbiol., 8, 147–204.
Carlton, B. C., and Gonzalez, J. J. M. (1984). “Plasmids and delta-endotoxin production in different subspecies of Bacillus thuringiensis.” Plasmid, 11, 246–252.
Crickmore, N., Zeigler, D. R., Feitelson, J., Schnepf, E., Rie, J. V., Lereclus, D., Baum, J., and Dean, D. H. (1998). “Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins.” Microbiol. Mol. Biol. Rev., 62(3), 807–813.
De Barjac, H. (1978). “A new subspecies of Bacillus thuringiensis very toxic for mosquitoes; Bacillus thuringiensis var israelensis serotype 14.” C. R. Seances Acad. Sci., Ser. D, 286, 797–800.
Dulmage, H. T. O. P. B., Rehnborg, C. S., and Hansen, G. D. (1971). “A proposed standardized bioassay for formulation of Bacillus thuringiensis based on the international unit.” J. Invertebr Pathol., 18, 240–245.
Fisher, S. H. (1993). “Utilisation of amino acids and other nitrogen containing compounds.” Bacillus subtilis and other gram positive bacteria, American Society for Microbiology, Washington, D.C.
Goldberg, L., and Margalit, J. (1977). “A bacterial spore demonstrating rapid larvicidal activity against Anopheles serengotti, Uranotaenia unguiculata, Culex univittalus, Aedes aegypti, and Culex pipiens.” Mosq. News, 37, 355–358.
Hitchins, A. D., Greene, R. A., and Slepecky, R. A. (1972). “Effect of carbon source on size and associated properties of Bacillus megaterium spores.” J. Bacteriol., 110, 392–401.
Hodges, N. A., and Brown, M. R. W. (1975). Properties of Bacillus megaterium spores formed under conditions of nutrient limitation, American Society of Microbiology, Washington, D.C.
Höfte, H., Seurinck, J., Houtven, A. V., and Vaeck, M. (1987). “Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera.” Nucleic Acids Res., 15, 7183–7783.
Lachhab, K., Tyagi, R. D., and Valéro, J. R. (2001). “Production of Bacillus thuringiensis biopesticides using wastewater sludge as a raw material: Effect of inoculum and sludge solids concentrations.” Process Biochem. (Oxford, U.K.), 37(2), 197–208.
Lecadet, M. M., Frachon, E., Cosmao-Dumanoir, V., Ripouteau, H., Hamon, S., Laurent, P., and Thiery, I. (1999). “Updating the H-antigen classification of Bacillus thuringiensis.” J. Appl. Microbiol., 86, 660–672.
Levinson, H. S., and Hyatt, M. T. (1964). “Effect of sporulation medium on heat resistance, chemical composition, and germination of Bacillus megaterium spores.” J. Bacteriol., 87, 876–886.
Luthy, P., Cordier, J., and Fisher, H. (1982). Bacillus thuringiensis as a bacterial insecticide: Basic consideration and application, in microbial and viral pesticides, E. Kurstaki, ed., Marcel Dekker, New York.
Mc Morran, A. (1965). “A synthetic diet of the Spruce budworm, Choristoneura fumiferana (Clems).” Can. Entomol., 97, 58–62.
Murell, W. G. (1967). “The biochemistry of the bacterial endospore.” Adv. Microb. Physiol., 1, 133–251.
Murell, W. G., and Warth, A. D. (1965). “Composition and heat resistance of bacterial spores.” Spores III, L. L. Campbell and H. O. Halvorson, eds., American Society of Microbiology, Ann Arbor, Mich.
Qasim, R. S. (1982). Wastewater treatment plants: Planning, design, and operations, Holt, Rinhart and Winston, New York.
Rowe, G. E., and Margaritis, A. (1987). “Bioprocess development in the production of bioinsecticides by Bacillus thuringiensis.” Crit. Rev. Biotechnol., 6(1), 87–127.
Sachdeva, V., Tyagi, R. D., and Valéro, J. R. (1999). “Production of biopesticides as a novel method of wasterwater sludge utilization/disposal.” Water Sci. Technol., 42, 211–216.
Salama, H. S., Foda, M. S., Dulmage, H. T., and El-Sharaby, A. (1983). “Novel fermentation media for production of delta-endotoxine for Bacillus thuringiensis.” J. Invertebr Pathol., 41, 8–19.
Slepecky, R. A., and Foster, J. W. (1959). “Alterations in metal content of spores of Bacillus megaterium and the effect on some spores properties.” J. Bacteriol., 78, 117–123.
Sreekrishnan, T. R., Tyagi, R. D., Blais, J. F., and Campbell, P. G. C. (1993). “Kinetics of heavy metal bioleaching from sewage sludge. I: Effects of process parameters.” Water Res., 27, 1641–1651.
Therien, A. G., and Blostein, R. (2000). “Mechanisms of sodium pump regulation.” Am. J. Physiol.: Cell Physiol., 279(3), C541–C566.
Tirado-Montiel, M. L., Tyagi, R. D., and Valero, J. R. (1998). “Production of Bacillus thuringiensis biopesticides using waste material.” Bioconversion of wastes materials to industrial products, Blackie Academic, New York.
Tirado-Montiel, M. L., Tyagi, R. D., and Valero, J. R. (2001). “Wastewater treatment sludge as a raw material for the production of Bacillus thuringiensis biopesticides.” Water Res., 35(16), 3807–3816.
Van Rie, J., Jansens, S., Hofte, H. H., Degheele, D., and van Mallaert, H. (1989). “Specificity of Bacillus thuringiensis delta-endotoxins: Importance of specific receptors on the brush border membrane of the midgut of target insects.” Eur. J. Biochem., 186, 1378–1385.
Vidyarthi, A. S., Tyagi, R. D., and Valéro, J. R. (2001). “Effect of surface-active agents on the production of biopesticides using wastewater sludge as raw material.” Water Sci. Technol., 44(10), 253–259.
Yang, X. M., and Wang, S. S. (1998). “Development of Bacillus thuringiensis fermentation and process control from a pratical perspective.” Biotechnol. Appl. Biochem., 28, 95–98.
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Published In
Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management
Volume 11 • Issue 2 • April 2007
Pages: 123 - 129
Copyright
© 2007 ASCE.
History
Received: Oct 12, 2006
Accepted: Oct 19, 2006
Published online: Apr 1, 2007
Published in print: Apr 2007
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