Changes in Polyhydroxy-Alkanoates (PHAs) during Enhanced Biological Phosphorus Removal with Dairy Industrial Wastewater
Publication: Journal of Environmental Engineering
Volume 135, Issue 11
Abstract
Using the industrial wastewater from a dairy plant, the performance of enhanced biological phosphorus removal (EBPR) with complex organic substances was evaluated. A laboratory-scale sequencing batch reactor (SBR) was operated and the organic loading rate in total chemical oxygen demand (tCOD) increased gradually from 200– in three steps. As the organic loading increased, the food to microorganism ratio increased from 0.16–0.27 (g-tCOD/g-MLVSS d). When it increased over , the effluent phosphorus concentration fluctuated, showing an unstable EBPR activity. During the anaerobic condition, higher fraction of poly-3-hydroxyvalerate (PHV) was observed and the ratio of PHV to poly-3-hydroxybuyrate (PHB) production ranged . PHV was produced faster and used later than PHB. By applying fluorescent in situ hybridization (FISH) technique, the percentage of Rhodocyclus-related bacteria to the total cell counts was monitored as an indicator of phosphorus accumulating organisms (PAOs). The population accounted for at low organic loading rate and stayed at the same level as the organic loading rate increased.
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Acknowledgments
This work was funded by the nine dairies in Wisconsin through Wisconsin Department of Natural Resources (WDNR) and Wisconsin Milk Marketing Board (WMMB) (Grant No. UNSPECIFIEDUW0202). The writers thank the field engineers and operators in surveying site for helpful assistance as well as Richard Reichardt at WDNR for their valuable input to this study.UNSPECIFIED
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Information
Published In
Journal of Environmental Engineering
Volume 135 • Issue 11 • November 2009
Pages: 1213 - 1220
Copyright
© 2009 ASCE.
History
Received: Jun 11, 2007
Accepted: Jun 18, 2009
Published online: Oct 15, 2009
Published in print: Nov 2009
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