Impact of Initial pH and Linoleic Acid (C18:2) on Hydrogen Production by a Mesophilic Anaerobic Mixed Culture
Publication: Journal of Environmental Engineering
Volume 134, Issue 2
Abstract
Hydrogen production from glucose using a mixed anaerobic culture was assessed under batch conditions by adjusting the initial pH and adding linoleic acid (LA). At an initial pH of 5.0, hydrogen ( glucose) was detected after the first glucose injection in the controls (no LA added). At the latter initial pH and in cultures fed LA, the yield reached a maximum of glucose when glucose was injected again. Hydrogen was detected after the second glucose injection in cultures with the initial pH adjusted to 5.0 and 6.0. In cultures receiving LA and adjusted to pH 7.6, the hydrogen yield reached glucose when glucose was injected again. Acetate, propionate, and butyrate were detected under all conditions; however, the quantities were variable and dependent on the conditions examined. In comparison to the amount of volatile fatty acids produced, relatively low quantities of alcohols (ethanol, i-propanol, n-butanol, and i-butanol) were detected during the initial lag phase of .
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Acknowledgments
Financial support for this work was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC)NSERC and the University of Windsor.
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© 2008 ASCE.
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Received: Nov 27, 2006
Accepted: Jul 27, 2007
Published online: Feb 1, 2008
Published in print: Feb 2008
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