TECHNICAL PAPERS
Feb 1, 2007

Nitric Oxide Emission and Soil Microbial Activities in Toluene Contaminated Soil

Publication: Journal of Environmental Engineering
Volume 133, Issue 2

Abstract

The purpose of this research is to consider a novel concept for measuring and monitoring (M&M) the presence and levels of contaminants in soil. Current M&M techniques include direct sensing for the target contaminant and the surrogate indicators of respired O2 and CO2 . The method suggested here is based on nitric oxide (NO). It is an alternative M&M method that could become quicker, easier, more reliable, and less expensive than the other M&M methods being used currently. NO emission from toluene contaminated soil and soil microbial activity were investigated to understand the NO-toluene-soil microbial relationships using fluorescent in situ hybridization molecular technique combined by enzymatic method. The relationships have been analyzed by the comparison of experimental measurements coming from analysis of variance statistical analysis, referring to toluene concentrations. Relationships between NO emissions and the microbial activity were significantly correlated (P<0.01) with the level of toluene concentration and duration of toluene contamination. Thus, NO emissions are suggested as a useful indicator of microbial activity in toluene contaminated soils.

Get full access to this article

View all available purchase options and get full access to this article.

Acknowledgments

The writers would like to thank Dwina Martin from Duke University for her valuable comments and suggestions during the conduct of this research.

References

Aiello, L. (1999). “Sorption and desorption of nitric oxide in the soil matrix.” Master’s thesis, Duke Univ., Durham, N.C.
Alvarez, P. J. J., Anid, P. J., and Vogel, T. M. (1991). “Kinetics of aerobic biodegradation of benzene and toluene in sandy aquifer materials.” Biodegradation, 2(1), 43–51.
Böckelmann, U., Szewzyk, U., and Elisabeth, G. (2003). “A new enzymatic method for the detachment of particle associated soil bacterial.” J. Microbiol. Methods, 55(1), 201–211.
Borden, R. C., Gomez, C. A., and Becker, M. T. (1995). “Geochemical indicators of intrinsic bioremediation.” Ground Water, 33(2), 180–189.
Brisou, J. F. (1995). “Biofilms.” Methods for enzymatic release of microorganisms, CRC, Boca Raton, Fla.
Christensen, H., Hansen, M., and Sorensen, J. (1999). “Counting and size and classification of active soil bacteria by fluorescence in situ hybridization with and rRNA oligonucleotide probe.” Appl. Environ. Microbiol., 65(4), 1753–1761.
Conrad, R. (1990). “Flux of NOx between soil and atmosphere: Importance of soil microbial metabolism.” Denitrification in soil and sediment, N. P. Reusbech and J. Sorenson, eds., Plenum, New York, 105–128.
Davis, G. B., Johnston, C. D., Patterson, B. M., Barber, C., and Bennett, M. (1998). “Estimation of biodegradation rates using respiration tests during in situ bioremediation of weathered diesel NAPL.” Ground Water Monit. Rem., 18(2), 123–132.
Deyo, B. G., Robbins, G. A., and Binkhorst, G. K. (1993). “Use of portable oxygen and carbon dioxide detectors to screen soil gas for subsurface contamination.” Ground Water, 31(4), 598–604.
Dunn, J. (1977). “Soil survey of Orange County.” North Carolina, Dept. of Agriculture, Soil Conservation Service, in cooperation with the North Carolina Agricultural Experiment Station and the Orange County Board of Commissioners, N.C.
Ferris, M. J., Muyzer, G., and Ward, D. M. (1996). “Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.” Appl. Environ. Microbiol., 62(2), 340–346.
Flemming, H. C., and Wingender, J. (2001). “Relevance of microbial extracellular polymeric substances (EPSs). I: Structural and ecological aspects.” Water Sci. Technol., 43(6), 1–8.
Hahn, D., Amann, R. I., Ludwig, W., Akermans, A. D. L., and Schleifer, K. H. (1992). “Detection of micro-organisms in soil after in-situ hybridization with rRNA-targeted fluorescently labeled oligonucleotides.” J. Gen. Microbiol., 138(5), 879–887.
Hill, G. T., Mitkowski, N. A., Aldrich-Wolfe, L., Emele, L. R., Jurkonie, D. D., Ficke, A., Maldonado-Ramirez, S., Lynch, S. T., and Nelson, E. B. (2000). “Methods for assessing the composition and diversity of soil microbial communities.” Applied Soil Ecology, 15(1), 25–36.
Hinchee, R. E., and Ong, S. K. (1992). “A rapid in situ respiration test for measuring aerobic biodegradation rates of hydrocarbons in soil.” J. Air Waste Manage. Assoc., 42(10), 1305–1312.
Hugenholtz, P., and Pace, N. R. (1996). “Identifying microbial diversity in the natural environment: A molecular phylogenetic approach.” Trends Biotechnol., 14(6), 190–197.
Hutchinson, G. L., Guenzi, W. D., and Livingston, G. P. (1993). “Soil water controls on aerobic soil emissions of gaseous nitrogen oxides.” Soil Biol. Biochem., 25(1), 1–9.
Jousset, S., Tabachow, R. M., and Peirce, J. J. (2001). “Soil nitric oxide emissions from nitrification and denitrification.” J. Environ. Eng., 127(4), 322–328.
Leahy, J. G., and Colwell, R. R. (1990). “Microbial degradation of hydrocarbons in the environment.” Microbiol. Rev., 54(3), 305–315.
Li, D. X., and Lundegard, P. D. (1996). “Evaluation of subsurface oxygen sensors for remediation monitoring.” Ground Water Monit. Rem., 16(1), 106–111.
Liu, S., and Suflita, J. M. (1993). “Ecology and evolution of microbial populations for bioremediation.” Trends Biotechnol., 11(8), 344–352.
Margesin, R., Zimmerbauer, A., and Schinner, F. (2000). “Monitoring of bioremediation by soil biological activities.” Chemosphere, 40(4), 339–346.
Meli, S. M., Badalucco, L., English, L. C., and Hopkins, D. W. (2003). “Respiratory responses of soil micro-organisms to simple and complex organic substrate.” Biol. Fertil. Soils, 37(2), 96–101.
Milcic-Terzic, J., Lopez-Vidal, Y., Vrvic, M. M., and Saval, S. (2000). “Biodegradation potential assessment of microbial consortia isolated from a diesel-contaminated soil.” Water Sci. Technol., 42(5–6), 403–406.
Ormeci, B., Sanin, S. L., and Peirce, J. J. (1999). “Laboratory study of NO flux from agricultural soil: Effects of soil moisture, pH, and temperature.” J. Geophys. Res., [Atmos.], 104(D1), 1621–1629.
Rammon, D. A., and Peirce, J. J. (1999). “Biogenic nitric oxide emissions from wastewater land application.” Atmos. Environ., 33(13), 2115–2121.
Sutherland, I. W. (1995). “Polysaccharide lyases.” FEMS Microbiol. Rev., 16, 323–347.
Tabachow, R. M. (2002). “Nitric oxide emissions from toluene-contaminated soil: Chemiluminescent monitoring with comparison to microbial community analysis.” Ph.D. thesis, Duke Univ., Durham, N.C.
Thomas, J.-C., Desrosiers, M., St-Pierre, Y., Lirette, P., Bisaillon, J.-G., Beaudet, R., and Villemur, R. (1997). “Quantitative flow cytometric detection of specific microorganisms in soil samples using rRNA targeted fluorescent probes and ethidium bromide.” Cytometry, 27(3), 224–232.
Tucker, M. R., Mesick, J. K., and Carter, C. C. (1997). “Crop fertilization based on North Carolina soil tests.” North Carolina Dept. of Agricultural and Consumer Services, Agronomic Division.
U.S. Environmental Protection Agency (USEPA). (1996a). “Innovative treatment technologies: Annual status report (8th Ed.).” EPA-542-R-96-101, Office of Solid Waste and Emergency Response, Washington, D.C.
U.S. Environmental Protection Agency (USEPA). (1996b). “Method 8260B-Volatile organic compounds by gas chromatography/mass spectrometry (GC/MS).” Office of Solid Waste, Washington, D.C., ⟨http://www.epa.gov/epaoswer/hazwaste/test/8xxx.htm⟩.
U.S. Environmental Protection Agency (USEPA). (2001). “Treatment technologies for site cleanup: Annual status report (tenth edition).” EPA 542-R-01-019, Office of Solid Waste and Emergency Responses, Washington, D.C.
Van Eyk, J., and Vreeken, C. (1991). In situ bioremediation, R. E. Hinchee and R. F. Olfenbuttel, eds., Butterworth-Heinemann, 303–320.
Wheatley, R. E., Ritz, K., Crabb, D., and Caul, S. (2001). “Temporal variations in potential nitrification dynamics in soil related to differences in rates and types of carbon and nitrogen inputs.” Soil Biol. Biochem., 33(15), 2135–2144.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 133Issue 2February 2007
Pages: 237 - 244

History

Received: Dec 9, 2005
Accepted: Jul 31, 2006
Published online: Feb 1, 2007
Published in print: Feb 2007

Permissions

Request permissions for this article.

Authors

Affiliations

Ki Don Cho, Ph.D.
Dept. of Civil and Environmental Engineering, Duke Univ., Durham, NC 27708.
J. Jeffrey Peirce
Associate Professor, Dept. of Civil and Environmental Engineering, Duke Univ., Durham, NC 27708 (corresponding author).

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share