Simulating the Effect of Liquid on Cryptosporidium parvum Oocysts in Aquifer Material
Publication: Journal of Environmental Engineering
Volume 130, Issue 12
Abstract
The effects of liquid injection on the viability of Cryptosporidium parvum oocysts were evaluated. A laboratory study was designed to test the effects of saturated , freeze–thaw cycles and different freezing protocols on C. parvum oocysts in aquifer material. Oocysts were exposed to a saturated solution of at room temperature for 1-, 4-, 8-, and intervals and their viability was compared with controls. One- and three-cycle freeze–thaw experiments on oocyst survival were conducted. Inactivation of oocysts was assessed for: (1) rapid freezing and rapid thawing and (2) gradual freezing and rapid thawing. Exposure to 1 atm of in water at room temperature had a negligible effect on oocyst viability. Average oocyst viability after the one- and three-cycle freeze–thaw experiments was 24.7 and 2.7%, repsectively. The average oocyst viability associated with the rapid freeze–thaw and gradual freeze–thaw experiments was 11.3 and 26.2%, respectively. Freezing associated with injection of liquid into aquifers would be the factor inactivating oocysts; to cause a 3-log decrease in oocyst viability multiple injections may be required.
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Information & Authors
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Published In
Journal of Environmental Engineering
Volume 130 • Issue 12 • December 2004
Pages: 1547 - 1551
Copyright
Copyright © 2004 ASCE.
History
Published online: Nov 15, 2004
Published in print: Dec 2004
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