TECHNICAL PAPERS
Feb 14, 2003

Comparative Study of Two Bioassays for Applications in Influent Wastewater Toxicity Monitoring

Publication: Journal of Environmental Engineering
Volume 129, Issue 3

Abstract

Bioluminescent bacteria-based assays can be used for influent wastewater toxicity monitoring for biological wastewater treatment systems. The most thoroughly studied bioluminescent bacteria-based test is the Microtox® assay. However, the response to toxicants of Photobacterium phosphoreum, the marine bacterial strain used in this assay, is different from that of the activated sludge microorganisms. We developed a continuous influent wastewater monitoring system based on the bioluminescent bacterium Shk1, a genetically modified Pseudomonad isolated from the activated sludge in an industrial wastewater treatment plant. The Shk1 toxicity data were correlated with the Microtox® toxicity data for 79 organic compounds and the two toxicity assays were compared. The Shk1 assay is less sensitive than the Microtox® assay and could therefore be more suitable for influent wastewater toxicity monitoring.

Get full access to this article

View all available purchase options and get full access to this article.

References

Arretxe, M., Heap, J. M., and Christofi, N.(1997). “The effect of toxic discharges on ATP content in activated sludge.” Environ. Toxicol. Water Qual., 12(1), 23–29.
Azur, Environmental(1997). “Continuous water quality monitoring.” Poll. Eng., 29(13), 54.
Blum, D. J. W., and Speece, R. E.(1991). “A database of chemical toxicity to environmental bacteria and its use in interspecies comparisons and correlations.” Res. J. Water Pollut. Control Fed., 63(3), 198–207.
Castillo, G. C., Vila, I. C., and Neild, E.(2000). “Ecotoxicity assessment of metals and wastewater using multitrophic assays.” Environ. Toxicol., 15(5), 370–375.
Dutka, B. J., and Kwan, K. K.(1982). “Application of four bacterial screening procedures to assess changes in the toxicity of chemical in mixtures.” Environ. Pollut., 29(2), 125–134.
Elnabarawy, M. T., Robideau, R. R., and Beach, S. A.(1988). “Comparison of three rapid toxicity test procedures: Microtox®, Polytox®, and activated sludge respiration inhibition.” Tox. Assess., 3, 361–370.
Evans, M. R., Jordinson, G. M., Rawson, D. M., and Rogerson, J. G.(1998). “Biosensors for the measurement of toxicity of wastewaters to activated sludge.” Pestic. Sci., 54(4), 447–452.
Fochtman, P., Raszka, A., and Nierzedska, E.(2000). “The use of conventional bioassays, microbiotests, and some “rapid” methods in the selection of an optimal test battery for the assessment of pesticides toxicity.” Environ. Toxicol., 15(5), 376–384.
Hao, O. J., Shin, C. J., Lin, C. F., Jeng, F. T., and Chen, Z. C.(1996). “Use of Microtox tests for screening industrial wastewater toxicity.” Water Sci. Technol., 34(10), 43–50.
Ince, N. H., and Erdogdu, G.(1998). “Toxicity screening, assessment, and reduction in an industrial wastewater treatment plant.” Water Environ. Res., 70(6), 1170–1177.
Kahru, A., Põllumaa, L., Reiman, R., Rätsep, A., Liiders, M., and Maloveryan, A.(2000). “The toxicity and biodegradability of eight main phenolic compounds characteristic to the oil-shale industry wastewaters: A test battery approach.” Environ. Toxicol., 15(5), 431–442.
Kaiser, K. L. E.(1988). “Photobacterium phosphoreum toxicity bioassay. II. Toxicity data compilation.” Tox. Assess., 3, 195–237.
Kaiser, K. L. E., and Devillers, J. (1994). Handbooks of ecotoxicological data. Ecotoxicity of chemicals to photobacterium phosphoreum, Gordon and Breach, Langhorne, Pa., Vol. 2.
Kelly, C. J., Lajoie, C. A., Layton, A. C., and Sayler, G. S.(1999). “Bioluminescence reporter bacterium for toxicity monitoring in biological wastewater treatment systems.” Water Environ. Res., 71(1), 31–35.
Kong, Z., Vanrolleghem, P. A., and Verstraete, W.(1993). “An activated sludge-based biosensor for rapid IC50 estimation and on-line toxicity monitoring.” Biosens. Bioelectron., 8(1), 49–58.
Koopman, B., and Bitton, G. (1986). “Toxicant screening in wastewaters.” Toxicity testing using microorganisms, B. J. Dutka and G. Bitton, eds., CRC, Boca Raton, Fla., Vol. 2, 101–132.
Lin, C. F., Hao, O. J., and Jeng, F. T.(1994). “Microtox evaluation of industrial wastewaters.” Water Sci. Technol., 30(10), 97–106.
Logue, C. L., Koopman, B., Brown, G. K., and Bitton, G.(1989). “Toxicity screening in a large, municipal wastewater system.” J. Water Pollut. Control Fed., 61(5), 632–640.
Polybac Corporation (1986). “Polytox rapid toxicity test procedure.” Polybac Application Procedure, Allentown, Pa.
Ren, S., and Frymier, P. D. (2002a). “Toxicity estimation of phenolic compounds by bioluminescent bacteria.” J. Environ. Eng., in press.
Ren, S., and Frymier, P. D.(2002b). “Estimating the toxicities of organic compounds to bioluminescent bacteria and activated sludge.” Water Res., 36, 4406–4414.
Reteuna, C., Vasseur, P., Cabridenc, R., and Lepailleur, H.(1986). “Comparison of respiration and luminescent tests in bacterial toxicity assessment.” Tox. Assess., 1(2), 159–168.
Reteuna, C., Vasseur, P., and Cabridenc, R.(1989). “Performances of three bacterial assays in toxicity assessment.” Hydrobiologia, 188/189(2), 149–153.
Ribo, J. M.(1997). “Interlaboratory comparison studies of the luminescent bacteria toxicity bioassay.” Environ. Toxicol. Water Qual., 12(4), 283–294.
Sanchez, P. S., Sato, M. I. Z., Paschoal, C. M. R. B., Alves, M. N., and Furlan, E. V.(1988). “Toxicity assessment of industrial effluents from S. Paulo State, Brazil, using short-term microbial assays.” Tox. Assess., 3, 55–80.
Schultz, T. W., Sinks, G. D., and Bearden, A. P. (1998). Comparative QSAR, J. Devillers, ed., Taylor & Francis, New York, 51–109.
Slabbert, J. L.(1988). “Microbial toxicity assays used for water quality evaluation in South Africa.” Tox. Assess., 3, 101–115.
Sun, B., Nirmalakhandan, N., Hall, E., Wang, X. H., Prakash, J., and Maynes, R.(1994). “Estimating toxicity of organic chemicals to activated-sludge microorganisms.” J. Environ. Eng., 120(1), 1459–1469.
Toussaint, M. W., Shedd, T. R., Van der Schalie, W. H., and Leather, G. R.(1995). “A comparison of standard acute toxicity tests with rapid-screening toxicity tests.” Environ. Toxicol. Chem., 14(5), 907–915.
Walker, J. D.(1989). “Effects of chemicals on microorganisms.” J. Water Pollut. Control Fed., 61(6), 1077–1097.
Williamson, K. J., and Johnson, D. G.(1981). “A bacterial bioassay for assessment of wastewater toxicity.” Water Res., 15(3), 383–390.
Wong, K. Y., Zhang, M. Q., Li, X. M., and Lo, W.(1997). “A luminescence-based scanning respirometer for heavy metal toxicity monitoring.” Biosens. Bioelectron., 12(2), 125–133.
Wooten, S.(1997). “A checklist for buying a toxicity monitor for rapid wastewater screening.” Water Eng. Manage. , 144(1), 32–33.

Information & Authors

Information

Published In

Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 129Issue 3March 2003
Pages: 216 - 221

History

Received: Jan 14, 2002
Accepted: Apr 18, 2002
Published online: Feb 14, 2003
Published in print: Mar 2003

Permissions

Request permissions for this article.

Authors

Affiliations

S. Ren, M.ASCE
Postdoctoral Research Associate, Dept. of Chemical Engineering, 419 Dougherty Engineering, Univ. of Tennessee, Knoxville, TN 37996-2200.
P. D. Frymier
Associate Professor, Dept. of Chemical Engineering, 419 Dougherty Engineering, Univ. of Tennessee, Knoxville, TN 37996-2200.

Metrics & Citations

Metrics

Citations

Download citation

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

View Options

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Get Access

Access content

Please select your options to get access

Log in/Register Log in via your institution (Shibboleth)
ASCE Members: Please log in to see member pricing

Purchase

Save for later Information on ASCE Library Cards
ASCE Library Cards let you download journal articles, proceedings papers, and available book chapters across the entire ASCE Library platform. ASCE Library Cards remain active for 24 months or until all downloads are used. Note: This content will be debited as one download at time of checkout.

Terms of Use: ASCE Library Cards are for individual, personal use only. Reselling, republishing, or forwarding the materials to libraries or reading rooms is prohibited.
ASCE Library Card (5 downloads)
$105.00
Add to cart
ASCE Library Card (20 downloads)
$280.00
Add to cart
Buy Single Article
$35.00
Add to cart

Media

Figures

Other

Tables

Share

Share

Copy the content Link

Share with email

Email a colleague

Share