Biodegradation of Naphthalene-Contaminated Soils in Slurry Bioreactors
Publication: Journal of Environmental Engineering
Volume 127, Issue 8
Abstract
The effects of naphthalene concentrations and soil constituents (sand, clay, organic matter) on biodegrading naphthalene-contaminated soils using an acclimated Flavobacterium sp. were examined in continuously stirred slurry bioreactors. Soils with and without organic matter (1%) and kaolinite clay (20%) were used. Studies showed that sorption of naphthalene can be represented by the Freundlich isotherm. Among the soils investigated, clayey soil retarded the biodegradation process the most due to its desorption characteristics. Increasing the naphthalene contamination level from 500 mg/kg to 25,000 mg/kg doubled the biodegradation time in the slurry reactor with a soil loading of 10 g/L. A pseudo first-order kinetic was observed for naphthalene biodegradation, and the biodegradation constant was determined to be 0.20 mg/L/h. A kinetic model was developed to simulate the biodegradation of naphthalene in a continuously stirred batch slurry reactor and to understand the role of solubilization (solid phase naphthalene), desorption, and biodegradation in the removal of naphthalene. Predictions using the numerical model agreed with the experimental data.
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References
1.
American Water Works Association (AWWA). ( 1994). Standard methods for the examination of water and wastewater, 17th Ed., Denver, Co.
2.
Angley, J. T., Brusseau, M. L., and Miller, W. L. ( 1992). “Nonequilibrium sorption and aerobic biodegradation of dissolved alkylbenzenes during transport in aquifer material: Column experiments and evaluation of a coupled-process model.” Envir. Sci. and Technol., 26, 1404–1410.
3.
ASTM. ( 1999). Annual book of ASTM standards, Philadelphia, Pa.
4.
ASTM. ( 2001). “Standard test methods for moisture, ash and organic matter of peat and other organic soils.” D 2974-00, West Conshohocken, Pa.
5.
Buitron, G., and Capdeville, B. ( 1993). “Uptake rate and mineralization of hexadecane and naphthalene by a mixed aerobic culture.” Water Res., 27, 847–853.
6.
CRC. ( 1996). Critical reviews in analytical chemistry, CRC, Boca Raton, Fla.
7.
EPA. ( 1990). “Basic concepts of contaminant sorption at hazardous waste sites.” EPA 540/4-90/053, Washington, D.C.
8.
EPA. ( 1997). “Cleaning up the nation's waste sites: markets and technology trends.” EPA 542/R-96/005A, Washington, D.C.
9.
EPA. ( 1986). “Test methods for evaluating solid waste physical/chemical methods.” EPA SW-846, Washington, D.C.
10.
Fleming, J. T., Sanseverlno, J., and Sayler, G. S. ( 1993). “Quantitative relationship between naphthalene catabolic gene frequency and expression in predicting PAH degradation in soils at town gas manufacturing sites.” Envir. Sci. and Technol., 27, 1068–1074.
11.
Fu, C., Pfanstiel, S., Gao, C., and Yan, X. ( 1996). “Studies on contaminant biodegradation in slurry, wafer, and compacted soil tube reactors.” Envir. Sci. and Technol., 30, 743–750.
12.
Gamerdinger, A. P., Achin, R. S., and Traxler, R. W. ( 1995). “Effect of aliphatic phase liquids on naphthalene biodegradation in multiphase systems.” Envir. Quality, 24, 1150–1156.
13.
Geerdink, M. J., Loosdrecht, M. C., and Luyben, K. C. ( 1996). “Model for microbial degradation of nonpolar organic contaminants in a soil slurry reactor.” Envir. Sci. and Technol., 30, 779–786.
14.
Ghosh, D. R., and Keinath, T. M. ( 1994). “Effect of clay minerals present in aquifer soils on the adsorption and desorption of hydrophobic organic compounds.” Envir. Progress, 13, 51–59.
15.
Ghoshal, S., Ramaswami, A., and Luthy, R. G. ( 1996). “Biodegradation of naphthalene from coal tar and heptamethylnonane in mixed batch systems.” Envir. Sci. and Technol., 30, 1282–1291.
16.
Goldsmith, C. D., and Balderson, R. K. ( 1989). “Biokinetic constants of a mixed microbial culture with model diesel fuel.” Haz. Waste and Haz. Mat., 6, 145–154.
17.
Herbes, S. E., and Schwall, L. R. ( 1978). “Microbial transformation of polycyclic aromatic hydrocarbons in pristine and petroleum-contaminated sediments.” Appl. and Envir. Microbiology, 28, 306–316.
18.
Huesemann, M. H. ( 1995). “Predictive model for estimating the extent of petroleum hydrocarbon biodegradation in contaminated soils.” Envir. Sci. and Technol., 29, 7–18.
19.
Kan, A. T., and Tomson, M. B. ( 1990). “Effect of pH concentration on the transport of naphthalene in saturated aquifer media.” J. Contaminant Hydro., 5, 235–251.
20.
Kanga, S. A., Bonner, J. S., and Page, C. A. ( 1997). “Solubilization of naphthalene and methyl-substituted naphthalenes from crude oil using biosurfactants.” Envir. Sci. and Technol., 31, 556–561.
21.
Kiyohara, H., Takizawa, N., and Nagao, K. ( 1992). “Natural distribution of bacteria metabolizing many kinds of polycyclic aromatic hydrocarbons.” Fermentation and Bioengineering, 74, 49–51.
22.
Lauch, R. P., Herrmann, J. G., and Mahaffey, W. R. ( 1992). “Removal of creosote from soil bioslurry reactors.” Envir. Progress, 11, 265–271.
23.
Liu, Z., Jacobson, A. M., and Luthy, R. G. ( 1995). “Biodegradation of naphthalene in aqueous nonionic surfactant systems.” Appl. and Envir. Microbiology, 61, 145–151.
24.
Luthy, R. G., Dzombak, D. A., Peters, C. A., and Roy, S. B. ( 1994). “Remediating tar-contaminated soils at manufactured gas plant sites.” Envir. Sci. and Technol., 28, 266A–276A.
25.
Madsen, E. L., and Thomas, C. T. ( 1996). “In situ dynamics of aromatic hydrocarbons and bacteria capable of AH metabolism in a coal tar waste-contaminated soils.” Envir. Sci. and Technol., 30, 2412–2416.
26.
Magdaliniuk, S., Block, J. C., and Leyval, C. ( 1995). “Biodegradation of naphthalene in montmorillonite/polyacrylamide suspensions.” Water Sci. and Technol., 31, 85–94.
27.
Mihelcic, J. R., and Luthy, R. G. ( 1991). “Sorption and microbial degradation of naphthalene in soil-water suspensions under denitrification conditions.” Envir. Sci. and Technol., 25, 169–177.
28.
Miller, M. E., and Alexander, M. ( 1991). “Kinetics of bacterial degradation of benzylamine in a montmorillonite suspension.” Envir. Sci. and Technol., 25, 240–245.
29.
Piatt, J. J., Backhus, D. A., and Capel, P. D. ( 1996). “Temperature-dependent sorption on naphthalene, phenanthrene, and pyrene to low organic carbon aquifer sediments.” Envir. Sci. and Technol., 30, 751–760.
30.
Putcha, R. V., and Domach, M. M. ( 1993). “Fluorescence monitoring of polycyclic aromatic hydrocarbon biodegradation and effect of surfactant.” Envir. Progress, 12, 81–85.
31.
Rogers, J. A., Tedaldi, D. J., and Kavanaugh, M. C. ( 1993). “A screening protocol for bioremediation of contaminated soil.” Envir. Progress, 12, 146–156.
32.
Shen, J., and Bartha, R. ( 1994). “On-site bioremediation of soil contaminated by no. 2 fuel oil.” J. Int. Biodeterioration and Biodegradation, 33, 61–72.
33.
Vipulanandan, C., and Krishnan, S. ( 1993). “Leachability and biodegradation of high concentrations of phenol and o-chlorophenol.” Haz. Waste and Haz. Mat., 10, 27–47.
34.
Volkering, F., Breure, A. M., and Andel, J. G. ( 1995). “Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons.” Appl. and Envir. Microbiology, 61, 1699–1705.
35.
Williams, P. A., Catterall, F. A., and Murray, K. ( 1975). “Metabolism of naphthalene, 2-methylnaphthalene, salicylate, and benzoate by pseudomonas PG: Regulation of tangential pathways.” J. Bacteriology, 124, 679–685.
36.
Wilson, M. S., and Madsen, E. L. ( 1996). “Field extraction of a transient intermediary metabolite indicative of real time in situ naphthalene biodegradation.” Envir. Sci. and Technol., 30, 2099–2103.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 127 • Issue 8 • August 2001
Pages: 748 - 754
History
Received: Jun 1, 1999
Published online: Aug 1, 2001
Published in print: Aug 2001
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