Environmental Factors Affecting Selenite Reduction by a Mixed Culture
Publication: Journal of Environmental Engineering
Volume 127, Issue 2
Abstract
This study presents the results of batch experiments investigating selenite reduction by an enriched anaerobic mixed culture as a function of several environmental factors, including pH, temperature, different electron donors and acceptors, as well as initial selenite concentrations. The initial selenite reduction was a zero-order reaction and was inhibited at higher selenite concentrations (>33 mg Se/L). The optimal temperature/pH for microbial reduction occurred at 30°C and pH 7.2. Selenite reduction was affected in the presence of high concentrations of sulfate [45 times the Se(IV) molar concentration], significantly affected by nitrate [105 times the Se(IV) molar concentration], and completely inhibited by chromate and oxygen. Ethanol was the preferred carbon source for selenite removal, followed by acetate, citrate, lactate, and glucose. The selenite-acclimated culture also reduces selenate without any lag period.
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References
1.
American Public Health Association (APHA). ( 1985). Standard methods for the examination of water and wastewater, 16th Ed., New York.
2.
Burton, G. A., Giddings, T. H, DeBrine, P., and Fall, R. ( 1987). “High incidence of selenite-resistant bacteria from a site polluted with selenium.” Appl. Envir. Microbiol., 53(1), 185–188.
3.
Fan, T. W. M., Higashi, R. M., and Lane, A. N. ( 1998). “Biotransformations of selenium oxyanion by filamentous cyanophyte-dominated mat cultured from agricultural drainage waters.” Envir. Sci. and Technol., 32(20), 3185–3193.
4.
Fujita, M., Ike, M., Nishimoto, S., Takahashi, K., and Kashiwa, M. ( 1997). “Isolation and characterization of a novel selenate-reducing bacterium, Bacillus sp. SF-1.” J. Fermentation and Bioengrg., 83(6), 517–522.
5.
Gharieb, M. M., Wilkinson, S. C., and Gadd, G. M. ( 1995). “Reduction of selenium oxyanions by unicellular, polymorphic and filamentous fungi—cellular location of reduced selenium and implications for tolerance.” J. Industrial Microbiol., Hampshire, U.K., 14(3/4), 300–311.
6.
Lawson, S., and Macy, J. M. ( 1995). “Bioremediation of selenite in oil refinery wastewater.” Appl. Microbiol Biotechnol., Berlin, 43(4), 762–765.
7.
Levine, V. E. ( 1924). “The reduction properties of microorganisms with special reference to selenium compounds.” J. Bacteriol., 10(3), 217–261.
8.
Lortie, L., Gould, W. D., Rajan, S., McCready, R. G. L., and Cheng, K. J. ( 1992). “Reduction of selenate and selenite to elemental selenium by a Pseudomonas stutzeri isolate.” Appl. Envir. Microbiol., 58(12), 4042–4044.
9.
Losi, M. E., and Frankenberger, W. T. ( 1997). “Reduction of selenium oxyanions by Enterobacter coalcae strain SLD1a01: Reduction of selenate to selenite.” Envir. Toxicol. Chem., 16(9), 1851–1858.
10.
Lovley, D. R. ( 1993). “Anaerobes into heavy metal: Dissimilatory metal reduction in anoxic environments.” Trends in Ecology and Evolution, 8(6), 213–217.
11.
Macy, J. M., Lawson, S., and Demoll-Decker, H. ( 1993). “Bioremediation of selenium oxyanions in San-Joaquin drainage water using Thauera-selenatis in a biological reactor system.” Appl. Microbiol. Biotechnol., Berlin, 40(4), 588–594.
12.
Macy, J. M., Michel, T. A., and Kirsch, D. G. ( 1989). “Selenate respiration by a Pseudomonas species; a new mode of anaerobic respiration.” FEMS Microbiol. Lett., Amsterdam, The Netherlands, 61(1/2), 195–198.
13.
Merrill, D. T., Manzione, M. A., Peterson, J. J., Parker, D. S., Chow, W., and Hobbs, A. O. ( 1986). “Field evaluation of arsenic and selenium removal by iron coprecipitation.” J. Water Pollution Control Fedn., 58(1), 18–26.
14.
Murry, A. P. ( 1988). “Removal of selenate from water by chemical-reduction.” Ind. and Engrg. Chem. Res., 27(1), 187–191.
15.
Oremland, R. S., Hollibaugh, J. T., Maest, A. S., Presser, T. S., Miller, L. G., and Culbertson, C. W. ( 1989). “Selenate reduction to elemental selenium by anaerobic bacteria in sediments and culture: Biogeochemical significance of a novel, sulfate-independent respiration.” Appl. Envir. Microbiol., 55(9), 2333–2343.
16.
Oremland, R. S., Steinberg, N. A., Maest, A. S., Miller, L. G., and Hollibaugh, J. T. ( 1990). “Measurement of in situ rates of selenate removal by dissimilatory bacterial reduction in sediments.” Envir. Sci. and Technol., 24(8), 1157–1164.
17.
Rege, M. A., et al. ( 1999). “Selenium reduction by a denitrifying consortium.” Biotechnol. Bioengrg., 62(4), 479–484.
18.
Sanuki, S., Kojima, T., Arai, K., Nagaoka, S., and Majima, H. ( 1999). “Photocatalytic reduction of selenate and selenite solutions using TiO2 powders.” Metallurgical Materials Trans B, 30(1), 15–20.
19.
Sorg, T. J., and Logson, G. S. ( 1978). “Treatment technology to meet the interim primary drinking water regulations for inorganic: Part 2.” J. AWWA, 70(7), 379–393.
20.
Thompson-Eagle, E. T., Frankenberger, W. T., and Karlson, U. ( 1989). “Volatilization of selenium by Alternaria alternata.” Appl. Envir. Microbiol., 55(6), 1406–1413.
21.
Tokunaga, T. K., Pickering, I. J., and Brown, G. E. ( 1996). “Selenium transformation in ponded sediments.” Soil Sci. Soc. Am. J., 60(3), 781–790.
22.
Tomei, F. A., Barton, L. L., Lemanski, C. L., and Zocco, T. G. ( 1992). “Reduction of selenate and selenite to elemental selenium by Wolinella succinogenes.” Can. J. Microbiol., Ottawa, 38(12), 1328–1333.
23.
Tomei, F. A., Barton, L. L., Lemanski, C. L., Zocco, T. G., Fink, N. H., and Sillerud, L. O. ( 1995). “Transformation of selenate and selenite to elemental selenium by Desulfovibrio desulfuricans.” J. Industrial Microbiol., Hampshire, U.K., 14(3/4), 329–336.
24.
Tucker, M. D., Barton, L. L., and Thomson, B. M. ( 1998). “Reduction of Cr, Mo, Se and U by Desulfovibrio desulfuricans immobilized in polyacrylamide gels.” J. Industrial Microbiol., Hampshire, U.K., 20(1), 13–19.
25.
Zingaro, R. A., Dufner, D. C., Murphy, A. P., and Moody, C. D. ( 1997). “Reduction of oxoselenium anions by iron(II) hydroxide.” Envir. Int., Oxford, U.K., 23(3), 299–304.
Information & Authors
Information
Published In
Journal of Environmental Engineering
Volume 127 • Issue 2 • February 2001
Pages: 175 - 178
History
Received: Dec 7, 1999
Published online: Feb 1, 2001
Published in print: Feb 2001
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