Modeling Biooxidation of Iron in Packed-Bed Reactor
Publication: Journal of Environmental Engineering
Volume 125, Issue 2
Abstract
A model based on Monod kinetics and originally developed for use with rotating biological contactors was modified for use with a packed-bed column reactor. The reactor was filled with expanded polystyrene beads to immobilize chemolithotrophic bacteria and fed up to 570 mg L−1 (∼10 mM) ferrous iron [Fe(II)] in simulated acid mine drainage. A tracer study indicated changing behavior as a function of hydraulic residence time (HRT), with a transition from complete mix flow behavior to plug flow behavior as HRT decreased. The Fe(II) oxidation efficiency exceeded 95% until the HRT was reduced below 0.5 h. The reactor performance could be predicted with the model using estimates from the literature for û and Y. The experimentally determined half-saturation constant Ks was found to range from 5 to 12 mg L−1. The maximum volumetric capacity constant Rmax was estimated to be ∼360 mg Fe(II)h−1 L−1 beads under complete mix flow conditions but appeared to be as high as 724 mg Fe(II)h−1 L−1 beads as conditions approached plug flow at short HRTs.
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References
1.
Armentia, H., and Webb, C. ( 1992). “Ferrous sulphate oxidation using Thiobacillus ferrooxidans cells immobilized in polyurethane foam support particles.” Appl. Microbiol. Biotechnol., 36, 697–700.
2.
Arredondo, R., Garcia, A., and Jerez, C. A. ( 1994). “Partial removal of lipopolysaccharide from Thiobacillus ferrooxidans affects its adhesion to solids.” Appl. Envir. Microbiol., 60(8), 2846–2851.
3.
Daigger, G. T., and Grady, C. P. L., Jr. ( 1982). “An assessment of the role of physiological adaptation in the transient response of bacterial cultures.” Biotechnol. Bioengrg., 24, 1427–1444.
4.
Diz, H. R. ( 1997). “Chemical and biological treatment of acid mine drainage for the removal of heavy metals and acidity,” PhD dissertation, Virginia Polytechnic Institute and State University, Blacksburg, Va.
5.
Diz, H. R., and Novak, J. T. ( 1997). “Heavy metal removal in an innovative treatment system for acid mine drainage.” Proc., 29th Mid-Atlantic Industrial and Haz. Waste Conf., Technomic Publishing, Lancaster, Pa., 183–194.
6.
Diz, H. R., and Novak, J. T. (1998). “A fluidized bed for removing iron and acidity from acid mine drainage.”J. Envir. Engrg., ASCE, 124(8), 701–708.
7.
Grishin, S. I., and Tuovinen, O. H. ( 1989). “Scanning electron microscopic examination of Thiobacillus ferrooxidans on different support matrix materials in packed bed and fluidized bed bioreactors.” Appl. Microbiol. Biotechnol., 31, 505–511.
8.
Handbook of chemistry and physics . (1990). CRC, Boca Raton, Fla.
9.
Jensen, A. B., and Webb, C. ( 1995). “Ferrous sulphate oxidation using Thiobacillus ferrooxidans: A review.” Proc. Biochem., 30(3), 225–236.
10.
Jones, C. A., and Kelly, D. P. ( 1983). “Growth of Thiobacillus ferrooxidans on ferrous iron in chemostat culture: Influence of product and substrate inhibition.” J. Chem. Tech. Biotechnol., 33B, 241–261.
11.
Karamanev, D., and Nikolov, L. ( 1988). “Influence of some physicochemical parameters on bacterial activity of biofilm: Ferrous iron oxidation by Thiobacillus ferrooxidans.” Biotechnol. Bioengrg., 31, 295–299.
12.
Karamanev, D., and Nikolov, L. ( 1991). “A comparison between the reaction rates in biofilm reactors and free suspended cells bioreactors.” Bioproc. Engrg., 6, 127–130.
13.
LaCombe Barron, J., and Lueking, D. R. ( 1990). “Growth and maintenance of Thiobacillus ferrooxidans cells.” Appl. Envir. Microbiol., 56(9), 2801–2806.
14.
Pirt, S. J. ( 1982). “Maintenance energy: A general model for energy-limited and energy-sufficient growth.” Arch. Microbiol., 133, 300–302.
15.
Ramsay, B., Ramsay, J., de Tremblay, M. and Chavarie, C. ( 1988). “A method for the quantification of bacterial protein in the presence of jarosite.” Geomicrobiol. J., 6, 171–177.
16.
Silverman, M. P., and Lundren, D. G. ( 1959). “Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans.” J. Bacteriol., 78, 326–331.
17.
Singer, P. C., and Stumm, W. ( 1970). “Acidic mine drainage: The rate-determining step.” Sci., 167, 1121–1123.
18.
18th Ed. American Public Health Association, American Water Works Association, Water Environment Federation, Washington, D.C.
19.
Starkey, R. L. ( 1945). “Precipitation of ferric hydrate by iron bacteria.” Sci., 102, 532–533.
20.
Stumm, W., and Morgan, J. J. ( 1970). Aquatic chemistry—An introduction emphasizing chemical equilibria in natural waters. Wiley-Interscience, New York.
21.
Stumm, W., and Morgan, J. J. ( 1981). Aquatic chemistry—An introduction emphasizing chemical equilibria in natural waters, 2nd Ed., Wiley-Interscience, New York.
22.
Temple, K. L., and Colmer, A. R. ( 1951). “The autotrophic oxidation of iron by a new bacterium: Thiobacillus ferrooxidans.” J. Bacteriol., 62, 605–611.
23.
USEPA. ( 1976). “Development document for interim final effluent limitations guidelines and new source performance standards for the coal mining point source category. Rep. No. EPA 440/1-76/057a, U.S. Environmental Protection Agency. Wehrli, B. (1990). “Redox reactions of metal ions at mineral surfaces.” Aquatic chemical kinetics, W. Stumm, ed., Wiley, New York, 311–336.
24.
Wichlacz, P. L., and Unz, R. F. ( 1985). “Growth kinetics of attached iron-oxidizing bacteria.” Appl. Envir. Microbiol., 50, 460–467.
25.
Williamson, K. J., and McCarty, P. L. ( 1976). “A model of substrate utilization by bacterial films.” J. Water Pollution Control Fed., 48(1), 9–23.
Information & Authors
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Published In
Journal of Environmental Engineering
Volume 125 • Issue 2 • February 1999
Pages: 109 - 116
History
Received: Nov 20, 1997
Published online: Feb 1, 1999
Published in print: Feb 1999
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