Exopolysaccharide Control of Methane Oxidation in Landfill Cover Soil
Publication: Journal of Environmental Engineering
Volume 125, Issue 12
Abstract
The study objective was to examine whether a relationship exists between the accumulation of exopolymeric substances (EPS) in landfill cover soil and the gradual decline in biotic methane oxidation observed in laboratory soil columns sparged with synthetic landfill gas. A mathematical model that combined multicomponent gas diffusion along the vertical axis of the columns with biotic methane oxidation was used to predict vertical gas gradients in the columns. An initial trial assumed methane oxidizers were embedded in a thin base layer of biofilm coating the soil, and the model predictions fit experimental data from soil columns early in their operating period. A second trial modeled the same system with a thick EPS layer coating the base biofilm and limiting diffusion of gases into and out of the cells. Predictions from the latter trials fit experimental data from soil columns later in their operating period when lower methane consumption rates were observed. The model results suggest that EPS accumulation may regulate methane oxidation rates in landfill covers.
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References
1.
Amaral, J. A., and Knowles, R. (1995). “Growth of methanotrophs in methane and oxygen counter gradients.” FEMS Microbiol. Letters, 126, 215–220.
2.
Anthony, C. (1982). The biochemistry of methylotrophs. Academic Press, New York.
3.
Anthony, C. ( 1991). “Assimilation of carbon by methylotrophs.” Biology of methylotrophs, I. Goldberg and J. S. Roken, eds., Butterworth-Heinemann, Boston, 79–109.
4.
Arcangeli, J. P., and Arvin, J. (1997). “Modeling of the growth of a methanotrophic biofilm.” Water Sci. and Technol., 36, 199–204.
5.
Augenstein, D. C. (1990). “Greenhouse effect contributions of United States landfill methane.” Proc., 13th Annu. Landfill Gas Symp., Gov. Refuse Collection and Disposal Association (GRCDA), Lincolnshire, Ill., 95–125.
6.
Bender, M., and Conrad, R. (1995). “Effect of CH4 oxidation activity.” Soil Biol. and Biochem., 27, 1517–1527.
7.
Bilbo, C. M., Arvin, E., Holst, H., and Spliid, H. (1992). “Modeling the growth of methane-oxidizing bacteria in a fixed biofilm.” Water Res., 26, 301–309.
8.
Bird, R. B., Stewart, W. E., and Lightfoot, E. N. (1960). Transport phenomena. Wiley, New York.
9.
Boeckx, P., and Van Cleemput, O. V. (1996). “Methane oxidation in a neutral landfill cover soil: Influence of moisture content, temperature, and nitrogen-turnover.” J. Envir. Quality, 25, 178–183.
10.
Bogner, J. (1992). “Anaerobic burial of refuse in landfills: Increased atmospheric methane and implications for increased carbon storage.” Ecolog. Bull., 42, 98–108.
11.
Bogner, J., Spokas, K., Burton, E., Sweeney, R., and Corona, V. (1995). “Landfills as atmospheric methane sources and sinks.” Chemosphere, 31, 4119–4130.
12.
Bogner, J. E., Spokas, K. A., and Burton, E. A. (1997). “Kinetics of methane oxidation in a landfill cover soil: Temporal variations, a whole-landfill oxidation experiment, and modeling of net CH4 emissions.” Envir. Sci. and Technol., 31, 2504–2514.
13.
Borjesson, G., and Svensson, B. H. (1997). “Seasonal and diurnal methane emissions from a landfill and their regulation by methane oxidation.” Waste Mgmt. and Res., 15, 33–54.
14.
Bowman, J. P., Jimenez, L., Rosario, I., Hazen, T. C., and Sayler, G. S. (1993). “Characterization of the methanotrophic bacteria community present in a trichloroethylene-contaminated subsurface groundwater site.” Appl. and Envir. Microbiol., 59, 2380–2387.
15.
Characklis, W. G., and Marshall, K. C. ( 1990). “Biofilms: A basis for an interdisciplinary approach.” Biofilms, W. G. Characklis and K. C. Marshall, eds., Wiley, New York, 3–15.
16.
Chida, K., Shen, G., Kodama, T., and Minoda, Y. (1983). “Acidic polysaccharide production from methane by a new methane-oxidizing bacterium H-2.” Agric. and Biolog. Chem., 47, 275–280.
17.
Christensen, B. E., and Characklis, W. G. ( 1990). “Physical and chemical properties of biofilms.” Biofilms, W. G. Characklis and K. C. Marshall, eds., Wiley, New York, 93–130.
18.
Christensen, B. E., Kjosbakken, J., and Smidsrod, O. (1985). “Partial chemical and physical characterization of two extracellular polysaccharides produced by marine periphytic Pseudomonas sp. Strain NCMB 2021.” Appl. and Envir. Microbiol., 50, 837–845.
19.
Costerton, J. W., Geesey, G. G., and Cheng, K. J. (1978). “How bacteria stick.” Sci. Am., 238, 86–95.
20.
Czepiel, P. M., Mosher, B., Crill, P. M., and Harriss, R. C. (1996). “Quantifying the effect of oxidation on landfill methane emissions.” J. Geophys. Res., 101, 16721–16729.
21.
Darbyshire, J. F., Robertson, L., and Mackie, L. A. (1985). “A comparison of two methods of estimating the soil pore network available to protozoa.” Soil Biol. and Biochem., 17, 619–624.
22.
“Emissions of greenhouse gases in the United States 1996.” (1997). Pub. # DOE/EIA 0573(96), U.S. Department of Energy, Energy Information Administration, Washington, D.C.
23.
Fennell, D. E., Underhill, S. E., and Jewell, W. J. (1992). “Methanotrophic attached-film reactor development and biofilm characteristics.” Biotechnol. and Bioengrg., 40, 1218–1232.
24.
Fletcher, M. ( 1992). “Bacterial metabolism in biofilms.” Biofilms-science and technology, L. F. Melo, T. R. Bott, M. Fletcher, and B. Capdeville, eds., Kluwer Academic, Dordrecht, The Netherlands, 113–124.
25.
Graham, D. W., Chaudhary, J. A., Hanson, R. S., and Arnold, R. G. (1993). “Factors affecting competition between type I and type II methanotrophs in two-organism, continuous-flow reactors.” Microbial Ecol., 25, 1–17.
26.
Griffin, D. M. (1968). “A theoretical study relating the concentration and diffusion of oxygen to the biology of organisms in soil.” New Phytology, 67, 561–577.
27.
Gupta, R. P., and Swartzendruber, D. (1962). “Flow-associated reduction in the hydraulic conductivity of quartz sand.” Soil Sci. Soc. of America Proc., 26, 6–10.
28.
Hanson, R. S., and Hanson, T. E. (1996). “Methanotrophic bacteria.” Microbiolog. Rev., 60, 439–471.
29.
Harremoes, P. ( 1978). “Biofilm kinetics.” Water pollution microbiology, Vol. 2, R. Mitchell, ed., Wiley, New York, 71–109.
30.
Hilger, H. A., Wollum, A. G., and Barlaz, M. A. (1999a). “Landfill methane oxidation response to vegetation, fertilization and liming.” J. Envir. Quality, in press.
31.
Hilger, H. A., Cranford, D. F., and Barlaz, M. A. (1999b). “Microbial exopolymer production in landfill cover soil.” Soil Biol. and Biochem., in press.
32.
Hoeks, J. (1972). “Changes in composition of soil air leaks in natural gas mains.” Soil Sci., 113, 46–54.
33.
Hou, C. T., Laskin, A. I., and Patel, R. N. (1978). “Growth and polysaccharide production by Methylocystis parvus OBBP on methanol.” Appl. and Envir. Microbiol., 37, 800–804.
34.
Jensen, T. E., and Corpe, W. A. ( 1991). “Ultrastructure of methylotrophic microorganisms.” Biology of methlotrophs, I. Goldberg and J. S. Roken, eds., Butterworth-Heinemann, Boston, 39–75.
35.
Jones, H. A., and Nedwell, D. B. (1993). “Methane emission and methane oxidation in landfill cover soil.” FEMS Microbiol. Ecol., 102, 185–195.
36.
Kightley, D., Nedwell, D. B., and Cooper, M. (1995). “Capacity for methane oxidation in landfill cover soils measured in laboratory-scale soil microcosms.” Appl. and Envir. Microbiol., 61, 592–601.
37.
Lanzarone, N. A., and McCarty, P. L. (1990). “Column studies on methanotrophic degradation of trichloroethylene and 1,2-dichloroethane.” Groundwater, 28, 910–919.
38.
Large, P. J. (1983). Methylotrophy and methanogenesis. Van Nostrand Reinhold, Berkshire, U.K.
39.
Linton, J. D., Watts, P. D., Austin, R. M., Haugh, D. E., and Neikus, H. G. D. (1986). “The energetics and kinetics of extracellular polysaccharide production from methanol by microorganisms possessing different pathways of C1 assimilation.” J. General Microbiol., 132, 779–788.
40.
Liptay, K., Chanton, J., Czepiel, P., and Mosher, B. (1998). “Use of stable isotopes to determine methane oxidation in landfill cover soils.” J. Geophys. Res., 103, 8243–8250.
41.
Livingston, A. G. (1991). “Biodegradation of 3,4-dichloroaniline in a fluidized bed bioreactor and a steady-state biofilm kinetic model.” Biotechnol. and Bioengrg., 38, 260–272.
42.
Mancinelli, R. L., and McKay, C. P. (1985). “Methane-oxidizing bacteria in sanitary landfills.” Proc., 1st Symp. on Biotechnolog. Advances in Processing Municipal Wastes for Fuels and Chem., A. Antonopoulos, ed., Argonne National Laboratory, Minneapolis, Minn., 437–450.
43.
Matson, J. V., and Characklis, W. G. (1976). “Diffusion into microbial aggregates.” Water Res., 10, 877–885.
44.
Mozes, N., and Rouxhet, P. G. ( 1992). “Influence of surfaces on microbial activity.” Biofilms-science and technology, L. F. Melo, T. R. Bott, M. Fletcher, and B. Capdeville, eds., Kluwer Academic, Dordrecht, The Netherlands, 125–136.
45.
Paul, E. A., and Clark, F. E. (1989). Soil microbiology and biochemistry. Academic Press, San Diego, Calif.
46.
Postma, J., and van Veen, J. A. (1990). “Habitable pore space and survival of Rhizobium leguminosarum biovar trifolii introduced into soil.” Microbial Ecol., 19, 149–161.
47.
Ren, T., Amaral, J. A., and Knowles, R. (1997). “The response of methane consumption by pure cultures of methanotrophic bacteria to oxygen.” Can. J. Microbiol., 43, 95–928.
48.
Rittmann, B. E., and McCarty, P. L. (1980). “Model of steady-state-biofilm kinetics.” Biotechnol. and Bioengrg., 22, 2343–2357.
49.
Shareefdeen, Z., Baltzis, G. M., Oh, Y. S., and Bartha, R. (1993). “Biofiltration of methanol vapor.” Biotechnol. and Bioengrg., 41, 512–524.
50.
Siegrist, H., and Gujer, W. (1985). “Mass transfer mechanisms in a biofilm.” Water Res., 19, 1369–1378.
51.
Sly, L. I., Bryant, L. J., and Cox, J. M. (1993). “Development of a biofilter for the removal of methane from coal mine ventilation atmosphere.” Appl. Microbiol. and Biotechnol., 39, 400–404.
52.
Southgate, G., and Goodwin, P. M. (1989). “The regulation of exopolysaccharide production and of enzymes involved in C1 assimilation in Methylophilus methylotrophus.” J. General Microbiol., 135, 2859–2867.
53.
Stern, D. I., and Kaufmann, R. K. (1996). “Estimates of global anthropogenic methane emissions 1860–1993.” Chemosphere, 33, 159–176.
54.
Sundh, I., Mikkela, C., Nilsson, M., and Svensson, B. H. (1995). “Potential aerobic methane oxidation in a sphagnum-dominated peatland—controlling factors and relation to methane emission.” Soil Biol. and Biochem., 27, 829–837.
55.
Thorstenson, D. C., and Pollock, D. W. (1989). “Gas transport in unsaturated zones: Multicomponent systems and the adequacy of Fick's laws.” Water Resour. Res., 25, 477–507.
56.
Travis, B. J., and Rosenberg, N. D. (1997). “Modeling in situ bioremediation of TCE at Savannah River: Effects of product toxicity and microbial interactions on TCE degradation.” Envir. Sci. and Technol., 31, 3093–3102.
57.
Trulear, M. G. ( 1983). “Cellular reproduction and extracellular polymer formation in the development of biofilms,” PhD thesis, Montana State University, Bozeman, Mont.
58.
Whalen, S. C., Reeburgh, W. S., and Sandbeck, K. A. (1990). “Rapid methane oxidation in a landfill cover soil.” Appl. and Envir. Microbiol., 56, 3405–3411.
59.
Whittenbury, R., Colby, J., Dalton, H., and Reed, H. L. ( 1976). “Biology and ecology of methane oxidizers.” Microbial production and utilization of gases: (H2, CH4, CO), H. G. Schlegel, G. Gottschalk, and N. Pfennig, eds., Goltze KG, Gottingen, Germany, 281–292.
60.
Whittenbury, R., Phillips, K. C., and Wilkinson, J. F. (1970). “Enrichment, isolation and some properties of methane-utilizing bacteria.” J. General Microbiol., 61, 205–218.
61.
Wilhelm, E., Battino, R., and Wilcock, R. J. (1977). “Low-pressure solubility of gases in liquid water.” Chem. Rev., 77, 219–262.
62.
Williamson, K., and Chung, T. H. ( 1975). “Dual limitation of substrate utilization kinetics within bacterial films.” 49th Nat. Meeting, American Institute of Chemical Engineers, Houston, Tex.
63.
Williamson, K., and McCarty, P. L. (1976). “A model of substrate utilization by bacterial films.” J. Water Pollution Control Fedn., 48, 9–24.
64.
Wrangstadh, M., Conway, P. L., and Kjelleberg, S. (1986). “The production and release of an extracellular polysaccharide during starvation of a marine pseudomonas sp. and the effect thereof on adhesion.” Archives of Microbiol., 145, 220–227.
65.
Wyss, O., and Moreland, E. J. (1968). “Composition of the capsule of obligate hydrocarbon-utilizing bacteria.” Appl. and Envir. Microbiol., 16, 185.
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Published In
Journal of Environmental Engineering
Volume 125 • Issue 12 • December 1999
Pages: 1113 - 1123
History
Received: Nov 17, 1998
Published online: Dec 1, 1999
Published in print: Dec 1999
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