TECHNICAL PAPERS
Apr 1, 1998

Comparison of Autotrophic and Mixotrophic Biofilters for H2S Removal

Publication: Journal of Environmental Engineering
Volume 124, Issue 4

Abstract

We demonstrate that the facultative chemoautotroph Thiobacillus novellus CH 3 removes hydrogen sulfide (H2S) gas in continuous reactors under nutrient-limiting conditions. Extensive tests including removal characteristics, metabolic products, and removal efficiencies of H2S by T. novellus CH 3 were conducted in autotrophic and mixotrophic environments. The optimal pH value and temperature required to remove hydrogen sulfide are found to be pH 7 and 26°C. The biofilter had an H2S removal efficiency greater than 99.5% under the mixotrophic condition after 10-day operation. The results show that the maximum removal rate and saturation constant were 1.9 g S/d per kg bead and 69.2 ppm, respectively. The main metabolic product of H2S oxidation was determined to be sulfate, but the conversion ratio was dependent on the growth environment. These results suggest that the mixotrophic potential of T. novellus CH 3 biofilter provides a significant advantage in H2S removal over autotrophic biofilters.

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References

1.
American Public Health Association (APHA). (1992). Standard method: examination of water and wastewater, 18th Ed., American Public Health Association, New York, N.Y.
2.
Barth, C. L., Elliott, F. L., and Melvin, S. W.(1984). “Using odour control technology to support animal agriculture.”Trans. ASAE, 27(8), 859–864.
3.
Bohn, H.(1992). “Consider biofiltration for decontaminating gases.”Chem. Eng. Progress., 88, 35–40.
4.
Buisman, C. J. N., Geraats, B. G., Ijspeert, P., and Lettinga, G.(1990). “Optimization of sulfur production in a biotechnological sulfide-removing reactor.”Biotechnol. Bioengrg., 35, 50–56.
5.
Carlson, D. A., and Leisner, C. P.(1966). “Soil beds for the control of sewage odors.”J. Water Pollution Control Fed., 38(11), 829–834.
6.
Cho, K. S., Hirai, M., and Shoda, M.(1991a). “Degradation characteristics of hydrogen sulfide, methanethiol, dimethyl sulfide and dimethyl disulfide by Thiobacillus thioparus DW44 isolated from peat biofilter.”J. Ferment. Bioengrg., 71(6), 384–389.
7.
Cho, K. S., Kuniyoshi, I., Hirai, M., and Shoda, M.(1991b). “A newly isolated heterotrophic bacterium, Xanthomonas sp. DY 44, to oxidize hydrogen sulfide to polysulfide.”Biotechnol. Lett., 13(12), 923–928.
8.
Chung, Y. C., Huang, C., and Tseng, C. P.(1996a). “Operation optimization of Thiobacillus thioparus CH11 biofilter for hydrogen sulfide removal.”J. Biotechnol., 52, 31–38.
9.
Chung, Y. C., Huang, C., and Tseng, C. P. (1996b). “Microbial oxidation of hydrogen sulfide with biofilter.”J. Envir. Sci. and Health, A31(6), 1263–1278.
10.
Chung, Y. C., Huang, C., and Tseng, C. P.(1996c). “Biodegradation of hydrogen sulfide by a laboratory-scale immobilized Pseudomonas putida CH11 biofilter.”Biotechnol. Prog., 12, 773–778.
11.
Eby, H. J., and Wilson, G. B. (1969). “Poultry house dust, odour and their mechanical removal.”Agric. Waste Mgmt. Proc., Cornell Univ. Conf. on Agric. Waste Mgmt., Syracuse, N.Y., 303–308.
12.
EiKum, A. S., and Storhang, R. (1986). “Odour problems related to waste water and sludge treatment.”Odour prevention and control of organic sludge and livestock farming, V. C. Neilsen, J. H. Voorburg, and P. L. Hermite, eds., Elsevier Applied Science Publishers, London, England, 12–18.
13.
Hirai, M., Ohtake, M., and Shoda, M.(1990). “Removal kinetic of hydrogen sulfide, methanethiol and dimethyl sulfide by peat biofilters.”J. Ferment. Bioengrg., 70(5), 334–339.
14.
Huang, C., Chung, Y. C., and Hsu, B. M.(1996). “Hydrogen sulfide removal by immobilized autotrophic and heterotrophic bacterial in the bioreacter.”Biotechnol. Tech., 10(8), 595–600.
15.
Kokufuta, E., Matsumoto, W., and Nakamura, I.(1982). “Immobilization of Nitrosomonas eruopaea cells with polyelectrolyte complex.”Biotechnol. Bioengrg., 24, 1591–1603.
16.
Langenhove, H. V., Bendinger, B., Oberthur, R., and Schamp, N. (1992). “Organic sulfur compounds: persistent odorants in biological treatment of complex waste gases.”Biotechniques for air pollution abatement and odour control policies, A. J. Dragt and J. V. Ham, eds., Elsevier Press, Amsterdam, The Netherlands, 177–182.
17.
Lee, S. K., and Shoda, M. (1989). “Biological deodorization using activated carbon fabric as a carrier of microorganisms.”J. Ferment. Bioengrg. 68(6), 437–442.
18.
Leefeldt, R. H., and Matin, A.(1980). “Growth and physiology of Thiobacillus novellus under nutrient-limited mixed conditions.”J. Bacteriol., 142(2), 645–650.
19.
Leson, G., and Winer, A. M.(1991). “Biofiltration: an innovative air pollution control technology for VOC emission.”J. Air and Waste Mgmt. Assoc., 41(8), 1045–1054.
20.
Mannebeck, H. (1986). “Covering manure storing tanks to control odour.”Odour prevention and control of organic sludge and livestock farming, V. C. Neilsen, J. H. Voorburg, and P. L. Hermite, eds., Elsevier Applied Science Publishers, London, England, 188–192.
21.
Matin, A.(1978). “Organic nutrition of chemolithotrophic bacteria.”Annu. Rev. Microbiol., 32, 433–468.
22.
Matin, A., Schleiss, M., and Perez, R. C.(1980). “Regulation of glucose transport and metabolism in Thiobacillus novellus.”J. Bacteriol., 142(2), 639–644.
23.
Medina, V. F., Webster, T., and Devinny, J. S. (1995). “Treatment of gasoline residuals by granular activated carbon based biological filtration.”J. Envir. Sci. and Health, A30(2), 407–422.
24.
Ottengraf, S. P. P., and Van Den Oever, A. H. C.(1983). “Kinetics of organic compound removal from waste gases with a biological filter.”Biotechnol. Bioengrg., 25, 3089–3102.
25.
Perez, R. C., and Matin, A.(1980). “Growth of Thiobacillus novellus on mixed substrates (mixotrophic growth).”J. Bacteriol., 142(2), 633–638.
26.
Perez, R. C., and Matin, A.(1982). “Carbon dioxide assimilation by Thiobacillus novellus under nutrient-limited mixotrophic conditions.”J. Bacteriol., 150(3), 46–51.
27.
Rands, M. B., Cooper, D. E., Woo, C. P., Fletcher, G. C., and Rolfe, K. A.(1981). “Compost filters for h2s removal from anaerobic digestion and rendering exhausts.”J. Water Pollution Control Fed., 53(10), 185–189.
28.
Roth, S. H. (1993). Hydrogen sulfide. Handbook of hazardous material. Academic Press, Inc., New York, N.Y.
29.
Sublette, K. L., and Sylvester, N. D.(1987). “Oxidation of hydrogen sulfide by continuous cultures of Thiobacillus denitrificans.”Biotechnol. Bioengrg., 29, 753–758.
30.
Tanji, Y., Kanagawa, T., and Mikami, E.(1989). “Removal of dimethyl sulfide, methyl mercaptan, and hydrogen sulfide by immobilized Thiobacillus thioparus TK-m.”J. Ferment. Bioengrg., 67(4), 280–285.
31.
Thompson, M. A., Kelkar, U. G., and Vickers, J. C.(1995). “The treatment of groundwater containing hydrogen sulfide using microfiltration.”Desalination, 102(2), 287–291.
32.
Tiwaree, R. S., Cho, K. S., Kirai, M., and Shoda, M.(1992). “Biological deodorization of dimethyl sulfide using different fabrics as the carriers of microorganisms.”Appl. Biochem. Biotech., 32, 135–148.
33.
Vanhoorne, M., Rouck, A., and de Bacquer, D.(1995). “Epidemiological study of eye irritation by hydrogen sulphide and/or carbon disulphide exposure in viscose rayon workers.”Ann. Occup. Hyg., 39(3), 307–315.
34.
Yang, Y., and Allen, E. R.(1994). “Biofiltration control of hydrogen sulfide 1. Design and operational parameters.”J. Air Waste Mgmt. Assoc., 44, 863–868.

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Go to Journal of Environmental Engineering
Journal of Environmental Engineering
Volume 124Issue 4April 1998
Pages: 362 - 367

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Published online: Apr 1, 1998
Published in print: Apr 1998

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Authors

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Ying-Chien Chung
PhD, Nat. Sci. Council, Taipei, Taiwan, R.O.C.; formerly, Inst. of Envir. Engrg., Nat. Chiao Tung Univ., Hsinchu, Taiwan, R.O.C. 30039.
Chihpin Huang
Prof., Inst. of Envir. Engrg., Nat. Chiao Tung Univ., Hsinchu, Taiwan, R.O.C. 30039. Correspondence should be addressed to Chihpin Huang.
Jill Ruhsing Pan
Res., Inst. of Envir. Engrg., Nat. Chiao Tung Univ., Hsinchu, Taiwan, R.O.C. 30039.
Ching-Ping Tseng
Assoc. Prof., Inst. of Biol. Sci. and Technol., Nat. Chiao Tung Univ., Hsinchu, Taiwan, R.O.C. 30039.

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